PROTEIN SEQUENCE OF 27-55; 60-86 AND 206-225.
STRAIN=ATCC 19718 / IFO 14298;
PubMed=8163487 [NCBI, ExPASy, EBI, Israel, Japan]
Arciero D.M., Hooper A.B.;
"A di-heme cytochrome c peroxidase from Nitrosomonas europaea catalytically active in both the oxidized and half-reduced states.";
J. Biol. Chem. 269:11878-11886(1994).

Comments

CATALYTIC ACTIVITY: 2 ferrocytochrome c + H₂O₂ = 2 ferricytochrome c + 2 H₂O.
BIOPHYSICOCHEMICAL PROPERTIES:

Redox potential: E⁰ are +450 mV (low spin) and -260 mV (high spin);
SUBCELLULAR LOCATION: Periplasm (Probable).
PTM: Binds 2 heme groups per subunit.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AL954747; CAD85226.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A53573; A53573.

RefSeq

NP_841364.1; -.

3D structure databases

PDB

1IQC; X-ray; 1.80 A; A/B/C/D=27-334.

[ExPASy / RCSB / EBI]

PDBsum

1IQC; -.

ModBase

P55929.

Protein family/group databases

PeroxiBase

5004; NeDiHCcP.

Enzyme and pathway databases

BioCyc

NEUR228410:NE1315-MON; -.

Family and domain databases

InterPro

IPR009056; Cyt_c_monohaem.
IPR004852; CytCP_MauG.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.760.10; Cytochrome_c_R; 1.

Pfam

PF03150; CCP_MauG; 1.
Pfam graphical view of domain structure.

PROSITE

PS51007; CYTC; 2.
PROSITE graphical view of domain structure (profiles).

BLOCKS

P55929.

Genome annotation databases

1082261; -.

AL954747_GR; NE1315.

neu:NE1315; -.

fig|228410.1.peg.1263; -.

Phylogenomic databases

HOGENOM

P55929; -.

Genome annotation databases

CMR

P55929; NE1315.

Other

ProtoNet

P55929.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Complete proteome; Direct protein sequencing; Electron transport; Heme; Iron; Metal-binding; Oxidoreductase; Periplasm; Peroxidase; Repeat; Signal; Transport.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 26`	`26`
`CHAIN`	`27 334`	`308`	`Cytochrome c551 peroxidase.`	`PRO_0000006599`
`METAL`	`69 69`		`Iron (heme 1 axial ligand) (By similarity).`
`METAL`	`213 213`		`Iron (heme 2 axial ligand) (By similarity).`
`METAL`	`270 270`		`Iron (heme 1 axial ligand) (By similarity).`
`METAL`	`284 284`		`Iron (heme 2 axial ligand) (By similarity).`
`BINDING`	`65 65`		`Heme 1 (covalent) (By similarity).`
`BINDING`	`68 68`		`Heme 1 (covalent) (By similarity).`
`BINDING`	`209 209`		`Heme 2 (covalent) (By similarity).`
`BINDING`	`212 212`		`Heme 2 (covalent) (By similarity).`
`CONFLICT`	`77 77`		`D -> C (in Ref. 2; AA sequence).`
`HELIX`	`42 52`	`11`
`HELIX`	`55 57`	`3`
`STRAND`	`58 61`	`4`
`HELIX`	`65 68`	`4`
`TURN`	`71 74`	`4`
`STRAND`	`79 81`	`3`
`HELIX`	`85 87`	`3`
`HELIX`	`100 102`	`3`
`STRAND`	`104 107`	`4`
`STRAND`	`112 114`	`3`
`HELIX`	`115 124`	`10`
`TURN`	`126 129`	`4`
`HELIX`	`133 141`	`9`
`HELIX`	`144 154`	`11`
`HELIX`	`161 173`	`13`
`HELIX`	`181 186`	`6`
`HELIX`	`195 207`	`13`
`HELIX`	`209 211`	`3`
`TURN`	`215 218`	`4`
`STRAND`	`221 226`	`6`
`STRAND`	`228 230`	`3`
`HELIX`	`242 245`	`4`
`HELIX`	`248 250`	`3`
`STRAND`	`253 255`	`3`
`HELIX`	`262 264`	`3`
`STRAND`	`267 269`	`3`
`HELIX`	`277 289`	`13`
`HELIX`	`295 306`	`12`

Sequence information

Length: 334 AA [This is the length of the unprocessed precursor]

Molecular weight: 36633 Da [This is the MW of the unprocessed precursor]

CRC64: 44E67A23550A862C [This is a checksum on the sequence]

        10         20         30         40         50         60 
MIKRTLTVSL LSLSLGAMFA SAGVMAANEP IQPIKAVTPE NADMAELGKM LFFDPRLSKS 

        70         80         90        100        110        120 
GFISCNSCHN LSMGGTDNIT TSIGHKWQQG PINAPTVLNS SMNLAQFWDG RAKDLKEQAA 

       130        140        150        160        170        180 
GPIANPKEMA STHEIAEKVV ASMPQYRERF KKVFGSDEVT IDRITTAIAQ FEETLVTPGS 

       190        200        210        220        230        240 
KFDKWLEGDK NALNQDELEG YNLFKGSGCV QCHNGPAVGG SSYQKMGVFK PYETKNPAAG 

       250        260        270        280        290        300 
RMDVTGNEAD RNVFKVPTLR NIELTYPYFH DGGAATLEQA VETMGRIQLN REFNKDEVSK 

       310        320        330 
IVAFLKTLTG DQPDFKLPIL PPSNNDTPRS QPYE

P55929 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools