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UniProtKB/Swiss-Prot entry P56560

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name AOFB_BOVIN
Primary accession number P56560
Secondary accession numbers Q0P5K2 Q864W3
Integrated into Swiss-Prot on December 15, 1998
Sequence was last modified on January 23, 2007 (Sequence version 4)
Annotations were last modified on    July 22, 2008 (Entry version 56)
Name and origin of the protein
Protein name Amine oxidase [flavin-containing] B
Synonyms EC 1.4.3.4
Monoamine oxidase type B
MAO-B
Gene name
Name: MAOB
From
Bos taurus (Bovine) [TaxID: 9913] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; Pecora; Bovidae; Bovinae; Bos.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA].
Chung P.P., Vaidyanathan G., Lanier S.M.;
Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
[2]
 NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=Hereford;
TISSUE=Basal ganglia;
NIH - Mammalian Gene Collection (MGC) project;
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
[3]
 PROTEIN SEQUENCE OF 2-17, ACETYLATION AT SER-2, AND MASS SPECTROMETRY.
DOI=10.1006/prep.2000.1309; PubMed=11049757 [NCBI, ExPASy, EBI, Israel, Japan]
Newton-Vinson P., Hubalek F., Edmondson D.E.;
"High-level expression of human liver monoamine oxidase B in Pichia pastoris.";
Protein Expr. Purif. 20:334-345(2000).
[4]
 PROTEIN SEQUENCE OF 22-36; 53-70; 101-120; 259-279; 289-323; 327-346; 362-403; 420-431 AND 456-485.
PubMed=2719656 [NCBI, ExPASy, EBI, Israel, Japan]
Powell J.F., Hsu Y.-P.P., Weyler W., Chen S.A., Salach J., Andrikopoulos K., Mallet J., Breakefield X.O.;
"The primary structure of bovine monoamine oxidase type A. Comparison with peptide sequences of bovine monoamine oxidase type B and other flavoenzymes.";
Biochem. J. 259:407-413(1989).
[5]
 DETERMINATION OF PROTEIN-FAD RATIO.
TISSUE=Liver;
PubMed=2764901 [NCBI, ExPASy, EBI, Israel, Japan]
Weyler W.;
"Monoamine oxidase A from human placenta and monoamine oxidase B from bovine liver both have one FAD per subunit.";
Biochem. J. 260:725-729(1989).
Comments
  • FUNCTION: Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine (By similarity).
  • CATALYTIC ACTIVITY: RCH2NH2 + H2O + O2 = RCHO + NH3 + H2O2.
  • COFACTOR: FAD.
  • SUBUNIT: Monomer, homo- or heterodimer (containing two subunits of similar size). Each subunit contains a covalently bound flavin. Enzymatically active as monomer.
  • SUBCELLULAR LOCATION: Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic side.
  • MASS SPECTROMETRY: Mass=59163.5; Mass_error=10.0; Method=Electrospray; Range=2-520; Source=PubMed:11049757;.
  • SIMILARITY: Belongs to the flavin monoamine oxidase family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF217955; AAF23179.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC119941; AAI19942.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S07573; S07573.
RefSeq NP_808813.2; -.
UniGene Bt.22460
3D structure databases
HSSP P27338; 1GOS. [HSSP ENTRY / PDB]
SMR P56560; 3-501.
ModBase P56560.
Ontologies
GO
GO:0005741; Cellular component: mitochondrial outer membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0008131; Molecular function: amine oxidase activity (inferred from electronic annotation from EC).
QuickGo view.
Family and domain databases
InterPro IPR001613; Amineoxid_fl.
IPR002937; Amino_oxidase.
Graphical view of domain structure.
Pfam PF01593; Amino_oxidase; 1.
Pfam graphical view of domain structure.
PRINTS PR00757; AMINEOXDASEF.
BLOCKS P56560.
Genome annotation databases
Ensembl ENSBTAG00000001288; Bos taurus. [Contig view]
GeneID 338445; -.
KEGG bta:338445; -.
Phylogenomic databases
HOVERGEN P56560; -.
Other
ProtoNet P56560.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Acetylation; Direct protein sequencing; FAD; Flavoprotein; Membrane; Mitochondrion; Mitochondrion outer membrane; Oxidoreductase; Transmembrane.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   520  519     Amine oxidase [flavin-containing] B. PRO_0000099856
TOPO_DOM   2   489  488     Cytoplasmic (By similarity). 
TRANSMEM   490   516  27     Anchor for type IV membrane protein (By similarity). 
TOPO_DOM   517   520  4     Mitochondrial intermembrane (By similarity). 
COMPBIAS   36    52  17     Arg/Lys-rich (basic). 
SITE   156   156  1     Important for catalytic activity (By similarity). 
SITE   365   365  1     Important for catalytic activity (By similarity). 
SITE   382   382  1     Important for catalytic activity (By similarity). 
MOD_RES   2     2        N-acetylserine. 
MOD_RES   52    52        N6-acetyllysine (By similarity). 
MOD_RES   397   397        S-8alpha-FAD cysteine (By similarity). 
CONFLICT   3     3        S -> N (in Ref. 1; AAF23179). 
CONFLICT   270   270        M -> G (in Ref. 4; AA sequence). 
CONFLICT   286   286        I -> T (in Ref. 1; AAF23179). 
CONFLICT   297   299        SIV -> PIM (in Ref. 4; AA sequence). 
CONFLICT   307   307        R -> K (in Ref. 4; AA sequence). 
CONFLICT   341   341        I -> L (in Ref. 4; AA sequence). 
CONFLICT   344   344        I -> K (in Ref. 4; AA sequence). 
CONFLICT   400   400        S -> A (in Ref. 4; AA sequence). 
CONFLICT   478   485        TTTFLQRH -> STSSMMMP (in Ref. 4; AA sequence). 
CONFLICT   509   509        F -> Y (in Ref. 2; AAI19942). 
CONFLICT   520   520        I -> V (in Ref. 1; AAF23179). 
Sequence information
Length: 520 AA [This is the length of the unprocessed precursor] Molecular weight: 58421 Da [This is the MW of the unprocessed precursor] CRC64: A594889B4495C410 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MSSKCDVVVV GGGISGMAAA KLLHDSGLNV IVLEARDRVG GRTYTLRNQK VKYVDLGGSY 

        70         80         90        100        110        120 
VGPTQNHILR LSKELGLETY KVNEVERLIH HTKGKSYPFR GSFPSVWNPI TYLDHNNLWR 

       130        140        150        160        170        180 
TMDDMGREIP SDAPWKAPLA EQWDLMTMKE LLDKICWTES SKQLAILFVN LCVTAEIHEV 

       190        200        210        220        230        240 
SALWFLWYVK QCGGTTRIFS TSNGGQERKF VGGSGQVSER IMDLLGDRVK LERPVIHIDQ 

       250        260        270        280        290        300 
TGENVLVETL NHELYEAKYV ISAVPPVLGM KIHFNPPLPM MRNQLITRVP LGSVIKSIVY 

       310        320        330        340        350        360 
YKEPFWRNMD YCGSMIIEGE EAPVAYALDD TKPDGSYPAI IGFILAHKAR KLARLTKEER 

       370        380        390        400        410        420 
LKKLCDLYAK VLGSQEALHP VHYEEKNWCE EQYSGGCYTS YFPPGIMTQY GRVLRQPVGR 

       430        440        450        460        470        480 
IYFAGTETAT HWSGYMEGAV EAGERAAREI LHAMGKIPED EIWLPEPESV DVPAKPITTT 

       490        500        510        520 
FLQRHLPSVP GLLKLIGLTT IFSATALGFL AHKRGLLVRI
P56560 in FASTA format

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