[1]	NUCLEOTIDE SEQUENCE [MRNA]. DOI=10.1006/bbrc.1995.2601; PubMed=7488079 [NCBI, ExPASy, EBI, Israel, Japan] de Castro E., Nef S., Fiumelli H., Lenz S.E., Kawamura S., Nef P.; "Regulation of rhodopsin phosphorylation by a family of neuronal calcium sensors."; Biochem. Biophys. Res. Commun. 216:133-140(1995).
[2]	NUCLEOTIDE SEQUENCE [MRNA]. TISSUE=Brain; Lindemeier J.; Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases.
[3]	MYRISTOYLATION AT GLY-2, CALCIUM-BINDING, AND SUBCELLULAR LOCATION. DOI=10.1074/jbc.274.42.30258; PubMed=10514519 [NCBI, ExPASy, EBI, Israel, Japan] McFerran B.W., Weiss J.L., Burgoyne R.D.; "Neuronal Ca(2+) sensor 1. Characterization of the myristoylated protein, its cellular effects in permeabilized adrenal chromaffin cells, Ca(2+)-independent membrane association, and interaction with binding proteins, suggesting a role in rapid Ca(2+) signal transduction."; J. Biol. Chem. 274:30258-30265(1999).

Comments

FUNCTION: Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin.
SUBUNIT: Interacts with KCND2 (By similarity).
INTERACTION:
P84080:ARF1 (xeno); NbExp=1; IntAct=EBI-907774, EBI-449051;
P14100:PDE1A (xeno); NbExp=1; IntAct=EBI-907774, EBI-907809;
SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane; Peripheral membrane protein (By similarity). Note=Associated with Golgi stacks. Post-synaptic densities of dendrites, and in the pre-synaptic nerve terminal at neuromuscular junctions.
MISCELLANEOUS: Binds 3 calcium ions via the second, third and fourth EF-hand.
SIMILARITY: Belongs to the recoverin family.
SIMILARITY: Contains 4 EF-hand domains.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

L27421; AAA88510.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X82188; CAA57678.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_077342.1; -.

UniGene

Rn.62653

3D structure databases

SMR

P62168; 3-188.

ModBase

P62168.

Protein-protein interaction databases

IntAct

P62168; -.

Organism-specific databases

RGD

68417; Freq.

Gene expression databases

ArrayExpress

P62168; -.

GermOnline

ENSRNOG00000008761; Rattus norvegicus.

Ontologies

GO:0005794;	Cellular component: Golgi apparatus (inferred from electronic annotation from UniProtKB-KW).
GO:0016020;	Cellular component: membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0005509;	Molecular function: calcium ion binding (inferred from electronic annotation from InterPro).
GO:0008427;	Molecular function: calcium-dependent protein kinase inhibitor activity (traceable author statement from UniProtKB).
GO:0005515;	Molecular function: protein binding (inferred from physical interaction from IntAct).
QuickGo view.

Family and domain databases

InterPro

IPR011992; EF-Hand_type.
IPR002048; EF_hand_Ca_bd.
IPR001125; Recoverin.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.238.10; EF-Hand_type; 1.

Pfam

PF00036; efhand; 3.
Pfam graphical view of domain structure.

PRINTS

PR00450; RECOVERIN.

ProDom

PD000012; EF-hand; 1.
[Domain structure / List of seq. sharing at least 1 domain]

SMART

SM00054; EFh; 3.
SMART graphical view of domain structure.

PROSITE

PS00018; EF_HAND_1; 3.
PS50222; EF_HAND_2; 3.
PROSITE graphical view of domain structure (profiles).

ProtoNet

P62168.

Genome annotation databases

Ensembl

ENSRNOG00000008761; Rattus norvegicus. [Contig view]

GeneID

65153; -.

KEGG

rno:65153; -.

Phylogenomic databases

HOVERGEN

P62168; -.

Other

NextBio

613993; -.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Calcium; Golgi apparatus; Lipoprotein; Membrane; Myristate; Repeat.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed.`
`CHAIN`	`2 190`	`189`	`Neuronal calcium sensor 1.`	`PRO_0000073790`
`DOMAIN`	`24 59`	`36`	`EF-hand 1.`
`DOMAIN`	`60 95`	`36`	`EF-hand 2.`
`DOMAIN`	`96 131`	`36`	`EF-hand 3.`
`DOMAIN`	`144 179`	`36`	`EF-hand 4.`
`CA_BIND`	`73 84`	`12`	`1.`
`CA_BIND`	`109 120`	`12`	`2.`
`CA_BIND`	`157 168`	`12`	`3.`
`REGION`	`36 47`	`12`	`Ancestral calcium site 1.`
`LIPID`	`2 2`		`N-myristoyl glycine.`

Sequence information

Length: 190 AA [This is the length of the unprocessed precursor]

Molecular weight: 21879 Da [This is the MW of the unprocessed precursor]

CRC64: 9AF8E26A23F80D4F [This is a checksum on the sequence]

        10         20         30         40         50         60 
MGKSNSKLKP EVVEELTRKT YFTEKEVQQW YKGFIKDCPS GQLDAAGFQK IYKQFFPFGD 

        70         80         90        100        110        120 
PTKFATFVFN VFDENKDGRI EFSEFIQALS VTSRGTLDEK LRWAFKLYDL DNDGYITRNE 

       130        140        150        160        170        180 
MLDIVDAIYQ MVGNTVELPE EENTPEKRVD RIFAMMDKNA DGKLTLQEFQ EGSKADPSIV 

       190 
QALSLYDGLV

P62168 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools