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UniProtKB/Swiss-Prot entry P80319


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name DHE3_PYRFU
Primary accession number P80319
Secondary accession numbers None
Integrated into Swiss-Prot on June 1, 1994
Sequence was last modified on February 1, 1996 (Sequence version 2)
Annotations were last modified on    September 2, 2008 (Entry version 67)
Name and origin of the protein
Protein name Glutamate dehydrogenase
Synonyms GDH
EC 1.4.1.3
Gene name
Name: gdhA
Synonyms: gdh
OrderedLocusNames: PF1602
From
Pyrococcus furiosus [TaxID: 2261] [HAMAP proteome]
Taxonomy Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; Pyrococcus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
DOI=10.1016/0378-1119(93)90527-A; PubMed=8406037 [NCBI, ExPASy, EBI, Israel, Japan]
Eggen R.I.L., Geerling A.C.M., Waldkoetter K., Antranikian G., de Vos W.M.;
"The glutamate dehydrogenase-encoding gene of the hyperthermophilic archaeon Pyrococcus furiosus: sequence, transcription and analysis of the deduced amino acid sequence.";
Gene 132:143-148(1993).
[2]
PROTEIN SEQUENCE.
STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
DOI=10.1007/BF01891983; PubMed=8060497 [NCBI, ExPASy, EBI, Israel, Japan]
Maras B., Valiante S., Chiaraluce R., Consalvi V., Politi L., de Rosa M., Bossa F., Scandurra R., Barra D.;
"The amino acid sequence of glutamate dehydrogenase from Pyrococcus furiosus, a hyperthermophilic archaebacterium.";
J. Protein Chem. 13:253-259(1994).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
Weiss R.B., Dunn D.M., Robb F.T., Brown J.R.;
"The complete sequence of the Pyrococcus furiosus genome.";
Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases.
[4]
X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
DOI=10.1016/S0969-2126(01)00251-9; PubMed=8591026 [NCBI, ExPASy, EBI, Israel, Japan]
Yip K.S.P., Stillman T.J., Britton K.L., Artymiuk P.J., Baker P.J., Sedelnikova S.E., Engel P.C., Pasquo A., Chiaraluce R., Consalvi V., Scandurra R., Rice D.W.;
"The structure of Pyrococcus furiosus glutamate dehydrogenase reveals a key role for ion-pair networks in maintaining enzyme stability at extreme temperatures.";
Structure 3:1147-1158(1995).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M97860; AAA83390.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE010260; AAL81726.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T46971; JN0854.
RefSeq NP_579331.1; -.
3D structure databases
PDB
1GTM; X-ray; 2.20 A; A/B/C=2-420.[ExPASy / RCSB / EBI]
PDBsum 1GTM; -.
ModBase P80319.
Family and domain databases
InterPro IPR006095; Glu/Leu/Phe/Val_DHase.
IPR006096; Glu/Leu/Phe/Val_DHase_C.
IPR006097; Glu/Leu/Phe/Val_DHase_dimer.
IPR014362; Glu_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
PANTHER PTHR11606:SF2; GLFV_DH; 1.
Pfam PF00208; ELFV_dehydrog; 1.
PF02812; ELFV_dehydrog_N; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000185; Glu_DH; 1.
PRINTS PR00082; GLFDHDRGNASE.
PROSITE PS00074; GLFV_DEHYDROGENASE; 1.
BLOCKS P80319.
Genome annotation databases
GeneID 1469478; -.
GenomeReviews AE009950_GR; PF1602.
KEGG pfu:PF1602; -.
NMPDR fig|186497.1.peg.1649; -.
Phylogenomic databases
HOGENOM P80319; -.
Other
LinkHub P80319; -.
Genome annotation databases
CMR P80319; PF1602.
Other
ProtoNet P80319.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; NAD; NADP; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   420  420     Glutamate dehydrogenase. PRO_0000182757
NP_BIND   220   226  7     NAD (Potential). 
ACT_SITE   105   105         
CONFLICT   88    89        AW -> WA (in Ref. 2; AA sequence). 
CONFLICT   366   366        T -> K (in Ref. 2; AA sequence). 
HELIX   6    17  12      
HELIX   18    20  3      
HELIX   25    31  7      
STRAND   35    45  11      
STRAND   51    62  12      
STRAND   66    69  4      
STRAND   72    74  3      
HELIX   80    96  17      
STRAND   102   109  8      
HELIX   112   114  3      
HELIX   117   131  15      
HELIX   132   134  3      
TURN   137   139  3      
HELIX   150   164  15      
HELIX   170   173  4      
HELIX   179   181  3      
TURN   185   189  5      
HELIX   190   205  16      
STRAND   215   219  5      
HELIX   223   234  12      
STRAND   239   244  6      
STRAND   249   256  8      
HELIX   258   268  11      
STRAND   269   271  3      
STRAND   277   280  4      
HELIX   282   287  6      
STRAND   291   295  5      
HELIX   306   309  4      
STRAND   313   316  4      
STRAND   319   321  3      
HELIX   325   333  9      
STRAND   337   339  3      
HELIX   341   344  4      
HELIX   347   361  15      
HELIX   367   391  25      
HELIX   396   414  19      
Sequence information
Length: 420 AA [This is the length of the unprocessed precursor] Molecular weight: 47114 Da [This is the MW of the unprocessed precursor] CRC64: 673DB20F8764A93C [This is a checksum on the sequence]
        10         20         30         40         50         60 
MVEQDPYEIV IKQLERAAQY MEISEEALEF LKRPQRIVEV TIPVEMDDGS VKVFTGFRVQ 

        70         80         90        100        110        120 
HNWARGPTKG GIRWHPEETL STVKALAAWM TWKTAVMDLP YGGGKGGIIV DPKKLSDREK 

       130        140        150        160        170        180 
ERLARGYIRA IYDVISPYED IPAPDVYTNP QIMAWMMDEY ETISRRKTPA FGIITGKPLS 

       190        200        210        220        230        240 
IGGSLGRIEA TARGASYTIR EAAKVLGWDT LKGKTIAIQG YGNAGYYLAK IMSEDFGMKV 

       250        260        270        280        290        300 
VAVSDSKGGI YNPDGLNADE VLKWKNEHGS VKDFPGATNI TNEELLELEV DVLAPAAIEE 

       310        320        330        340        350        360 
VITKKNADNI KAKIVAEVAN GPVTPEADEI LFEKGILQIP DFLCNAGGVT VSYFEWVQNI 

       370        380        390        400        410        420 
TGYYWTIEEV RERLDKKMTK AFYDVYNIAK EKNIHMRDAA YVVAVQRVYQ AMLDRGWVKH 

P80319 in FASTA format

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