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[1]
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PROTEIN SEQUENCE OF 1-27.
TISSUE=Venom;
Tan C.H.,
Ang W.C.;
Submitted (MAY-1998) to UniProtKB.
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[2]
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PROTEIN SEQUENCE OF 27-107, FUNCTION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES, AND MASS SPECTROMETRY.
TISSUE=Venom;
DOI=10.1016/j.toxicon.2006.05.004; PubMed=16828829 [NCBI, ExPASy, EBI, Israel, Japan]
Tonismagi K.,
Samel M.,
Trummal K.,
Ronnholm G.,
Siigur J.,
Kalkkinen N.,
Siigur E.;
"L-amino acid oxidase from Vipera lebetina venom: isolation, characterization, effects on platelets and bacteria.";
Toxicon 48:227-237(2006).
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- FUNCTION: Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids (highest specificity towards L-Met, L-Trp, L-Leu followed by L-His, L-Phe, L-Arg and L-Ile. L-Gly, L-Ser, L-Thr, L-Pro, L-Cys, L-Asp are not oxidized). Inhibits both agonist-induced platelet aggregation. Has cytotoxic activity. Has an antibacterial activity against both Gram-negative (E.coli) and Gram-positive (B.subtilis) bacteria. May also have an ability to induce apoptosis and hemorrhage.
- CATALYTIC ACTIVITY: An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.
- COFACTOR: FAD.
- BIOPHYSICOCHEMICAL PROPERTIES:
| Kinetic parameters: |
KM=0.40 mM for L-Leu; | | KM=0.65 mM for L-Met; | | KM=0.17 mM for L-Trp; | | Temperature dependence: |
Thermostable between 4 and 25 degrees Celsius. At -20 degrees Celsius, the remaining activity is 20%. Heating at 70 degrees Celsius inactivates the enzyme in 15 minutes; | |
- SUBUNIT: Homodimer.
- SUBCELLULAR LOCATION: Secreted.
- TISSUE SPECIFICITY: Expressed by the venom gland.
- PTM: Glycosylated (By similarity).
- SIMILARITY: Belongs to the flavin monoamine oxidase family. FIG1 subfamily.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 107 AA [This is the length of the partial sequence of the unprocessed precursor] |
Molecular weight: 12435 Da [This is the MW of the partial sequence of the unprocessed precursor] |
CRC64: 576D2F829779B412 [This is a checksum on the sequence] |
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10 20 30 40 50 60
ADDKNPLEEC FREDDYEEFL EIAKNGLEGW YANLGPMRYP VKPSEEGKHD DIFAYEKFDE
70 80 90 100
IVGGMDKKFW EDDGIHGGKE TFCYSPMIQK PYQFQHFSEA LTAPVGR
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P81375 in FASTA format |
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