NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
STRAIN=cv. SS1000;
TISSUE=Etiolated seedling;
PubMed=9076987 [NCBI, ExPASy, EBI, Israel, Japan]
Bak S., Kahn R.A., Olsen C.E., Halkier B.A.;
"Cloning and expression in Escherichia coli of the obtusifoliol 14 alpha-demethylase of Sorghum bicolor (L.) Moench, a cytochrome P450 orthologous to the sterol 14 alpha-demethylases (CYP51) from fungi and mammals.";
Plant J. 11:191-201(1997).

Comments

FUNCTION: Catalyzes the 14-alpha demethylation of obtusifoliol to 4 alpha-methyl-5 alpha-ergosta-8,14,24(28)-trien-3 beta-ol.
CATALYTIC ACTIVITY: Obtusifoliol + 3 O₂ + 3 NADPH = 4-alpha-methyl-5-alpha-ergosta-8,14,24²⁸-trien-3-beta-ol + formate + 3 NADP⁺ + 4 H₂O.
COFACTOR: Heme group.
PATHWAY: Steroid metabolism; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6 .
SUBCELLULAR LOCATION: Membrane (Potential). Membrane; Single-pass membrane protein.
SIMILARITY: Belongs to the cytochrome P450 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U74319; AAC49659.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

T14820; T14820.

3D structure databases

HSSP

P77901; 1E9X. [HSSP ENTRY / PDB]

ModBase

P93846.

Organism-specific databases

Gramene

P93846; -.

Family and domain databases

InterPro

IPR001128; Cyt_P450.
IPR002403; Cyt_P450_E_grp-IV.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.630.10; Cyt_P450; 1.

PANTHER

PTHR19383; Cyt_P450; 1.

Pfam

PF00067; p450; 1.
Pfam graphical view of domain structure.

PRINTS

PR00465; EP450IV.
PR00385; P450.

PROSITE

PS00086; CYTOCHROME_P450; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Direct protein sequencing; Heme; Iron; Lipid synthesis; Membrane; Metal-binding; Methyltransferase; Monooxygenase; NADP; Oxidoreductase; Steroid biosynthesis; Sterol biosynthesis; Transferase; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 492`	`492`	`Cytochrome P450 51.`	`PRO_0000052013`
`TRANSMEM`	`12 32`	`21`	`Potential.`
`METAL`	`434 434`		`Iron (heme axial ligand) (By similarity).`

Sequence information

Length: 492 AA [This is the length of the unprocessed precursor]

Molecular weight: 55653 Da [This is the MW of the unprocessed precursor]

CRC64: 0C86160DFD812ECE [This is a checksum on the sequence]

        10         20         30         40         50         60 
MDLADIPQQQ RLMAGLALVV ATVIFLKLLL SFRSGGGKKR LPPTIPGAPV VGGLVKFMRG 

        70         80         90        100        110        120 
PIPMIREQYA ALGSVFTVPI ITRRITFLIG PEVSAHFFKG NEAEMSQQEV YRFNVPTFGP 

       130        140        150        160        170        180 
GVVFDVDYSV RQEQFRFFTE ALRANKLRSY VDQMVAEAEE YFSKWGESGT VDLKYELEHL 

       190        200        210        220        230        240 
IILTASRCLL GREVREKLFD DVSALFHDLD NGIQPISVLF PYLPIPAHKR RDKARARLAE 

       250        260        270        280        290        300 
IFATIIKSRK ASGQSEEDML QCFIDSKYKN GRPTTEGEVT GLLIAALFAG QHTSSITSTW 

       310        320        330        340        350        360 
TGAYMLRFKQ YFAEAVEEQK DVMKRHGDKI DHDILAEMDV LYRCIKEALR LHPPLIMLLR 

       370        380        390        400        410        420 
QSHSDFTVTT KEGKEYDIPK GHIVATSPSF ANRLPHIYKN PDSYDPDRFG PGREEDKAAG 

       430        440        450        460        470        480 
AFSYISFGGG RHGCLGEPFA YLQIKAIWTH LLRNFEFELV SPFPENDWNA MVVGIKGEVM 

       490 
VNYKRRKLVV DN

P93846 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools