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[1]
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NUCLEOTIDE SEQUENCE [MRNA], AND AUTOPROTEOLYTIC CLEAVAGE.
STRAIN=CD-1;
TISSUE=Kidney;
DOI=10.1074/jbc.272.13.8466; PubMed=9079674 [NCBI, ExPASy, EBI, Israel, Japan]
Valentini R.P.,
Brookhiser W.T.,
Park J.,
Yang T.,
Briggs J.,
Dressler G.,
Holzman L.B.;
"Post-translational processing and renal expression of mouse indian hedgehog.";
J. Biol. Chem. 272:8466-8473(1997).
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[2]
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NUCLEOTIDE SEQUENCE [MRNA] OF 76-411.
STRAIN=C57BL/6J;
TISSUE=Embryo;
DOI=10.1016/0092-8674(93)90627-3; PubMed=7916661 [NCBI, ExPASy, EBI, Israel, Japan]
Echelard Y.,
Epstein D.J.,
St Jacques B.,
Shen L.,
Mohler J.,
McMahon J.A.,
McMahon A.P.;
"Sonic hedgehog, a member of a family of putative signaling molecules, is implicated in the regulation of CNS polarity.";
Cell 75:1417-1430(1993).
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[3]
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SEQUENCE REVISION.
St Jacques B.;
Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases.
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[4]
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NUCLEOTIDE SEQUENCE [MRNA] OF 124-172.
PubMed=7720571 [NCBI, ExPASy, EBI, Israel, Japan]
Chang D.T.,
Lopez A.,
von Kessler D.P.,
Chiang C.,
Simandl B.K.,
Zhao R.,
Seldin M.F.,
Fallon J.F.,
Beachy P.A.;
"Products, genetic linkage and limb patterning activity of a murine hedgehog gene.";
Development 120:3339-3353(1994).
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- FUNCTION: Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP).
- SUBCELLULAR LOCATION: Indian hedgehog protein N-product: Cell membrane; Lipid-anchor; Extracellular side (By similarity). Note=The N-terminal peptide remains associated with the cell surface (By similarity).
- SUBCELLULAR LOCATION: Indian hedgehog protein C-product: Secreted, extracellular space (By similarity). Note=The C-terminal peptide diffuses from the cell (By similarity).
- TISSUE SPECIFICITY: In the embryo, detected in the developing gut, the growth zone of cartilage of developing long bones, epithelium and urogenital sinus. In the adult kidney, found in proximal convoluted and proximal straight tubule.
- DEVELOPMENTAL STAGE: Detected at 10 dpc in developing gut, at 14.5 days dpc in the cartilage primordium and in the developing urogenital sinus. Expression increases with gestional age in kidney and duodenum, becoming maximal in adulthood.
- PTM: The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity (By similarity).
- PTM: Cholesterylation is required for N-product targeting to lipid rafts and multimerization (By similarity).
- PTM: N-palmitoylation is required for N-product multimerization and full activity (By similarity).
- SIMILARITY: Belongs to the hedgehog family.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 411 AA [This is the length of the unprocessed precursor] |
Molecular weight: 45485 Da [This is the MW of the unprocessed precursor] |
CRC64: 08BE7AD8507C0D9B [This is a checksum on the sequence] |
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10 20 30 40 50 60
MSPAWLRPRL RFCLFLLLLL LVPAARGCGP GRVVGSRRRP PRKLVPLAYK QFSPNVPEKT
70 80 90 100 110 120
LGASGRYEGK IARSSERFKE LTPNYNPDII FKDEENTGAD RLMTQRCKDR LNSLAISVMN
130 140 150 160 170 180
QWPGVKLRVT EGWDEDGHHS EESLHYEGRA VDITTSDRDR NKYGLLARLA VEAGFDWVYY
190 200 210 220 230 240
ESKAHVHCSV KSEHSAAAKT GGCFPAGAQV RLENGERVAL SAVKPGDRVL AMGEDGTPTF
250 260 270 280 290 300
SDVLIFLDRE PNRLRAFQVI ETQDPPRRLA LTPAHLLFIA DNHTEPAAHF RATFASHVQP
310 320 330 340 350 360
GQYVLVSGVP GLQPARVAAV STHVALGSYA PLTRHGTLVV EDVVASCFAA VADHHLAQLA
370 380 390 400 410
FWPLRLFPSL AWGSWTPSEG VHWYPQMLYR LGRLLLEEST FHPLGMSGAG S
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P97812 in FASTA format |
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