[1]	NUCLEOTIDE SEQUENCE [MRNA]. DOI=10.1016/S0014-5793(99)01138-2; PubMed=10481044 [NCBI, ExPASy, EBI, Israel, Japan] Schroeder G., Unterbusch E., Kaltenbach M., Schmidt J., Strack D., Schroeder J.; "Light-induced cytochrome P450-dependent enzyme in indole alkaloid biosynthesis: tabersonine 16-hydroxylase."; FEBS Lett. 458:97-102(1999).
[2]	CHARACTERIZATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE. PubMed=12228585 [NCBI, ExPASy, EBI, Israel, Japan] St Pierre B., De Luca V.; "A cytochrome P-450 monooxygenase catalyzes the first step in the conversion of tabersonine to vindoline in Catharanthus roseus."; Plant Physiol. 109:131-139(1995).
[3]	TISSUE SPECIFICITY. DOI=10.1111/j.1365-313X.2005.02557.x; PubMed=16262708 [NCBI, ExPASy, EBI, Israel, Japan] Murata J., Luca V.D.; "Localization of tabersonine 16-hydroxylase and 16-OH tabersonine-16-O-methyltransferase to leaf epidermal cells defines them as a major site of precursor biosynthesis in the vindoline pathway in Catharanthus roseus."; Plant J. 44:581-594(2005).

Comments

FUNCTION: Involved in the biosynthesis of vindoline, a precursor of vinblastine and vincristine.
CATALYTIC ACTIVITY: Tabersonine + NADPH + O₂ = 16-hydroxytabersonine + NADP⁺ + H₂O.
COFACTOR: Heme group (By similarity).

BIOPHYSICOCHEMICAL PROPERTIES:

Kinetic parameters:	K_M=11 µM for tabersonine;
	K_M=14 µM for NADPH;
	Note=concentrations of tabersonine greater than 30 µM are inhibitory;
pH dependence:	Optimum pH is 7.5 in phosphate buffer and 8.0 in TRIS-HCl;
Temperature dependence:	Stable at 30 degrees Celsius;

PATHWAY: Alkaloid biosynthesis; vindoline biosynthesis; vindoline from tabersonine: step 1/6 .
SUBCELLULAR LOCATION: Endoplasmic reticulum.
TISSUE SPECIFICITY: Predominantly expressed in young leaves of mature plants. Low expression in roots and flowers, but not detected in stems and old leaves. Found predominantly in leaf epidermis.
DEVELOPMENTAL STAGE: Peak of expression after light treatment at 9 days of seedling development.
INDUCTION: Inhibited by cytochrome c, CO, clotrimazole, miconazole, tricliphane, flusilazole and tetcyclasis, and with a lower efficiency, by 1-phenylimidazole and piperonylbutoxide. Not inhibited by potassium cyanide and sodium azide.
SIMILARITY: Belongs to the cytochrome P450 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AJ238612; CAB56503.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

P14779; 1JPZ. [HSSP ENTRY / PDB]

ModBase

P98183.

Enzyme and pathway databases

BioCyc

MetaCyc:MON-7709; -.

Family and domain databases

InterPro

IPR001128; Cyt_P450.
IPR002401; Cyt_P450_E_grp-I.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.630.10; Cyt_P450; 1.

PANTHER

PTHR19383; Cyt_P450; 1.

Pfam

PF00067; p450; 1.
Pfam graphical view of domain structure.

PRINTS

PR00463; EP450I.
PR00385; P450.

PROSITE

PS00086; CYTOCHROME_P450; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Endoplasmic reticulum; Heme; Iron; Metal-binding; Monooxygenase; NADP; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`<1 495`	`>495`	`Tabersonine 16-hydroxylase.`	`PRO_0000310728`
`COMPBIAS`	`490 493`	`4`	`Poly-Ser.`
`NON_TER`	`1 1`

Sequence information

Length: 495 AA [This is the length of the partial sequence of the unprocessed precursor]

Molecular weight: 56590 Da [This is the MW of the partial sequence of the unprocessed precursor]

CRC64: D769003236430161 [This is a checksum on the sequence]

        10         20         30         40         50         60 
LLFCFILSKT TKKFGQNSQY SNHDELPPGP PQIPILGNAH QLSGGHTHHI LRDLAKKYGP 

        70         80         90        100        110        120 
LMHLKIGEVS TIVASSPQIA EEIFRTHDIL FADRPSNLES FKIVSYDFSD MVVSPYGNYW 

       130        140        150        160        170        180 
RQLRKISMME LLSQKSVQSF RSIREEEVLN FIKSIGSKEG TRINLSKEIS LLIYGITTRA 

       190        200        210        220        230        240 
AFGEKNKNTE EFIRLLDQLT KAVAEPNIAD MFPSLKFLQL ISTSKYKIEK IHKQFDVIVE 

       250        260        270        280        290        300 
TILKGHKEKI NKPLSQENGE KKEDLVDVLL NIQRRNDFEA PLGDKNIKAI IFNIFSAGTE 

       310        320        330        340        350        360 
TSSTTVDWAM CEMIKNPTVM KKAQEEVRKV FNEEGNVDET KLHQLKYLQA VIKETLRLHP 

       370        380        390        400        410        420 
PVPLLLPREC REQCKIKGYT IPSKSRVIVN AWAIGRDPNY WIEPEKFNPD RFLESKVDFK 

       430        440        450        460        470        480 
GNSFEYLPFG GGRRICPGIT FALANIELPL AQLLFHFDWQ SNTEKLNMKE SRGVTVRRED 

       490 
DLYLTPVNFS SSSPA

P98183 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools