[1]	NUCLEOTIDE SEQUENCE [MRNA]. PubMed=1883330 [NCBI, ExPASy, EBI, Israel, Japan] Cannons A.C., Iida N., Solomonson L.P.; "Expression of a cDNA clone encoding the haem-binding domain of Chlorella nitrate reductase."; Biochem. J. 278:203-209(1991).

Comments

FUNCTION: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.
CATALYTIC ACTIVITY: Nitrite + NAD⁺ + H₂O = nitrate + NADH.
COFACTOR: Binds 1 FAD per subunit.
COFACTOR: Binds 1 heme group per subunit.
COFACTOR: Binds 1 molybdenum-pterin group per subunit.
SUBUNIT: Homodimer.
SIMILARITY: Belongs to the nitrate reductase family.
SIMILARITY: Contains 1 cytochrome b5 heme-binding domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X56771; CAA40090.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S17197; S17197.

3D structure databases

HSSP

P04166; 1B5M. [HSSP ENTRY / PDB]

ModBase

Q01170.

Family and domain databases

InterPro

IPR001199; Cyt_B5.
IPR005066; MoCF_OxRdtse_dimer.
IPR008335; Mopterin_OxRdtase_euk.
IPR000572; OxRdtase_Mopterin-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.10.120.10; Cyt_B5; 1.
G3DSA:2.60.40.650; MoCF_oxrdtse_dimer; 1.

Pfam

PF00173; Cyt-b5; 1.
PF03404; Mo-co_dimer; 1.
Pfam graphical view of domain structure.

PRINTS

PR00363; CYTOCHROMEB5.
PR00407; EUMOPTERIN.

ProDom

PD000612; Cyt_B5; 1.
[Domain structure / List of seq. sharing at least 1 domain]

PROSITE

PS00191; CYTOCHROME_B5_1; 1.
PS50255; CYTOCHROME_B5_2; 1.
PS00559; MOLYBDOPTERIN_EUK; PARTIAL.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

FAD; Flavoprotein; Heme; Iron; Metal-binding; Molybdenum; NAD; Nitrate assimilation; Oxidoreductase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`<1 >318`	`>318`	`Nitrate reductase [NADH].`	`PRO_0000166053`
`DOMAIN`	`216 291`	`76`	`Cytochrome b5 heme-binding.`
`METAL`	`251 251`		`Iron (heme axial ligand) (By similarity).`
`METAL`	`274 274`		`Iron (heme axial ligand) (By similarity).`
`NON_TER`	`1 1`
`NON_TER`	`318 318`

Sequence information

Length: 318 AA [This is the length of the partial sequence of the unprocessed precursor]

Molecular weight: 34830 Da [This is the MW of the partial sequence of the unprocessed precursor]

CRC64: E60D82FE1E98292A [This is a checksum on the sequence]

        10         20         30         40         50         60 
RPPVTEVESQ NYYHFHDNRV LPSHVDEALA NSEGWWYKPD FIINDLNVQS AIGYPAHEEV 

        70         80         90        100        110        120 
VPLVAGTYAV RGYARGHGNK IIRCEVSLDD GKSWRLGSVT HEGQPTEYGK HWGWVWWSLE 

       130        140        150        160        170        180 
VPIAELLTTP EIICRAWDSS MNTQPNTFTW NVMGMMNNCC YRVKIHPRQT TDGRFALQFE 

       190        200        210        220        230        240 
HPTIAGPTVG GWMNRAEDVA AAAAVTVAPP PAPAGAKSFT MAEVETHTTM ESAWFVVDGK 

       250        260        270        280        290        300 
VYDATPFLKD HPGGADSILL VAGIDATDEF NAIHSLKAKK QLLEYYIGEL AEEGQEAAAS 

       310 
DRATPGPAAA IGTAVPVA

Q01170 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools