[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. STRAIN=Hereford; TISSUE=Placenta; NIH - Mammalian Gene Collection (MGC) project; Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.

Comments

CATALYTIC ACTIVITY: 5,10-methylenetetrahydrofolate + NAD⁺ = 5,10-methenyltetrahydrofolate + NADH.
CATALYTIC ACTIVITY: 5,10-methenyltetrahydrofolate + H₂O = 10-formyltetrahydrofolate.
COFACTOR: Magnesium (By similarity).
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Mitochondrion (By similarity).
MISCELLANEOUS: This NAD-dependent bifunctional enzyme has very different kinetic properties than the larger NADP-dependent trifunctional enzyme and is unique in that it requires formation of an enzyme-magnesium complex to allow binding of NAD (By similarity).
SIMILARITY: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BC120249; AAI20250.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001069223.1; -.

UniGene

Bt.6685

3D structure databases

ModBase

Q0P5C2.

Family and domain databases

InterPro

IPR000672; THF_DHase/CycOHase.
Graphical view of domain structure.

Pfam

PF00763; THF_DHG_CYH; 1.
PF02882; THF_DHG_CYH_C; 1.
Pfam graphical view of domain structure.

PRINTS

PR00085; THFDHDRGNASE.

ProDom

PD002300; THFDhg/Cyc_hydro; 1.
[Domain structure / List of seq. sharing at least 1 domain]

PROSITE

PS00766; THF_DHG_CYH_1; 1.
PS00767; THF_DHG_CYH_2; 1.

BLOCKS

Q0P5C2.

Genome annotation databases

Ensembl

ENSBTAG00000004881; Bos taurus. [Contig view]

GeneID

517539; -.

KEGG

bta:517539; -.

Phylogenomic databases

HOVERGEN

Q0P5C2; -.

Other

ProtoNet

Q0P5C2.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Hydrolase; Magnesium; Mitochondrion; Multifunctional enzyme; NAD; One-carbon metabolism; Oxidoreductase; Transit peptide.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 35`	`35`	`Mitochondrion (By similarity).`
`CHAIN`	`36 350`	`315`	`Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial.`	`PRO_0000305902`

Sequence information

Length: 350 AA [This is the length of the unprocessed precursor]

Molecular weight: 37767 Da [This is the MW of the unprocessed precursor]

CRC64: D71A67DFE1FEE05F [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAAASFITSL VTRLLRSAQS GRLHQRPFHL SAVRNEAVVI SGRKLAEQIK QEVRQEVEEW 

        70         80         90        100        110        120 
VASGNKRPHL SVVLVGENPA SQSYVLNKTR AAASVGINSE TILKPASISE EELLNLINKL 

       130        140        150        160        170        180 
NNDDNVDGLL VQLPLPEHID ERKVCNAVSP DKDVDGFHVI NVGRMCLDQC SMLPATPWGV 

       190        200        210        220        230        240 
WEIIKRTGIP TLGKNVVVAG RSKNVGMPIA MLLHTDGAHE RPGGDATVTI SHRYTPKEEL 

       250        260        270        280        290        300 
KKHTALADIV ISAAGIPNLI TADMIKEGAA VIDVGINRIQ DPITAKPKLV GDVDFEGVKK 

       310        320        330        340        350 
KAGYITPVPG GVGPMTVAML MKNTIIAAKK VLRLEEQEVL KSKELGVASN

Q0P5C2 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools