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UniProtKB/Swiss-Prot entry Q10258


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MTHR1_SCHPO
Primary accession number Q10258
Secondary accession numbers None
Integrated into Swiss-Prot on October 1, 1996
Sequence was last modified on October 1, 1996 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 55)
Name and origin of the protein
Protein name Methylenetetrahydrofolate reductase 1
Synonym EC 1.5.1.20
Gene name
Name: met9
ORFNames: SPAC56F8.10
From
Schizosaccharomyces pombe (Fission yeast) [TaxID: 4896] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1002/yea.877; PubMed=12112238 [NCBI, ExPASy, EBI, Israel, Japan]
Naula N., Walther C., Baumann D., Schweingruber M.E.;
"Two non-complementing genes encoding enzymatically active methylenetetrahydrofolate reductases control methionine requirement in fission yeast Schizosaccharomyces pombe.";
Yeast 19:841-848(2002).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 38366 / 972;
DOI=10.1038/nature724; PubMed=11859360 [NCBI, ExPASy, EBI, Israel, Japan]
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
"The genome sequence of Schizosaccharomyces pombe.";
Nature 415:871-880(2002).
[3]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-355, AND MASS SPECTROMETRY.
DOI=10.1021/pr7006335; PubMed=18257517 [NCBI, ExPASy, EBI, Israel, Japan]
Wilson-Grady J.T., Villen J., Gygi S.P.;
"Phosphoproteome analysis of fission yeast.";
J. Proteome Res. 7:1088-1097(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
CU329670; CAA93581.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T38920; T38920.
RefSeq NP_593224.1; -.
3D structure databases
HSSP P00394; 1B5T. [HSSP ENTRY / PDB]
ModBase Q10258.
Enzyme and pathway databases
BioCyc SPOM-XXX-01:SPOM-XXX-01-000918-MON; -.
Organism-specific databases
GeneDB_Spombe SPAC56F8.10; -.
Gene expression databases
ArrayExpress Q10258; -.
Ontologies
GO
GO:0005829; Cellular component: cytosol (inferred from direct assay from GeneDB_SPombe).
GO:0005634; Cellular component: nucleus (inferred from direct assay from GeneDB_SPombe).
GO:0004489; Molecular function: methylenetetrahydrofolate reductase (NADPH) activity (inferred from direct assay from GeneDB_SPombe).
GO:0006555; Biological process: methionine metabolic process (inferred from direct assay from GeneDB_SPombe).
QuickGo view.
Family and domain databases
InterPro IPR004621; Fadh2_euk.
IPR003171; Mehydrof_redctse.
Graphical view of domain structure.
Pfam PF02219; MTHFR; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR00677; fadh2_euk; 1.
BLOCKS Q10258.
Genome annotation databases
GeneID 2542993; -.
KEGG spo:SPAC56F8.10; -.
NMPDR fig|4896.1.peg.3194; -.
Other
ProtoNet Q10258.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; FAD; Flavoprotein; NADP; Oxidoreductase; Phosphoprotein.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   603  603     Methylenetetrahydrofolate reductase 1. PRO_0000190253
MOD_RES   355   355        Phosphoserine. 
Sequence information
Length: 603 AA [This is the length of the unprocessed precursor] Molecular weight: 69012 Da [This is the MW of the unprocessed precursor] CRC64: 38519FEE783D331F [This is a checksum on the sequence]
        10         20         30         40         50         60 
MKISDKLLHP DWKEKVTYSY EFFPPKTSTG VQNLYNRIDR MKTWGRPMFV DVTWGAGGTS 

        70         80         90        100        110        120 
SELTPGIVNV IQTDFEVDTC MHLTCTNMST EMIDAALKRA HETGCRNILA LRGDPVKDTD 

       130        140        150        160        170        180 
WTEGESGFRY ASDLVRYIRT HYNDEFCIGV AGYPEGYSPD DDIDESIKHL KLKVDEGADF 

       190        200        210        220        230        240 
IVTQMFYDVD NFIAWVDKVR AAGINIPIFP GIMPIQAWDS FIRRAKWSGV KIPQHFMDTL 

       250        260        270        280        290        300 
VPVKDDDEGV RERGVELIVE MCRKLIASGI TRLHFYTMNL EKAVKMIIER LGLLDENLAP 

       310        320        330        340        350        360 
IVDTNNVELT NASSQDRRIN EGVRPIFWRT RNESYVSRTD QWDELPHGRW GDSRSPAFGE 

       370        380        390        400        410        420 
FDAIRYGLRM SPKEITTSWG SPKSYSEIGD LFARYCEKKI SSLPWSDLPI SDEADLIRDQ 

       430        440        450        460        470        480 
LLSMNRNAFL TINSQPALNG EKSSHPVFGW GPPNGYVFQK PYVEFFVHPS LLNELKETVK 

       490        500        510        520        530        540 
KLNSVSYFVT NKNGDLDTNS QYEIPNAVTW GVFPNREIIQ PTIVESTSFL AWKDEAYSLG 

       550        560        570        580        590        600 
MEWANAYSPD SISRKLLVSM MKEWFLCVIV DNDFQNGQSL FDVFNKMRSL KDIHPELYYA 


NAS 

Q10258 in FASTA format

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