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UniProtKB/Swiss-Prot entry Q10N21


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name APX1_ORYSJ
Primary accession number Q10N21
Secondary accession numbers P93404 Q84QS3 Q8GZZ2
Integrated into Swiss-Prot on September 11, 2007
Sequence was last modified on August 22, 2006 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 22)
Name and origin of the protein
Protein name L-ascorbate peroxidase 1, cytosolic
Synonyms APXa
EC 1.11.1.11
OsAPx01
Gene name
Name: APX1
OrderedLocusNames: Os03g0285700, LOC_Os03g17690
ORFNames: OSJNBa0013D02.10, OsJ_009999
From
Oryza sativa subsp. japonica (Rice) [TaxID: 39947] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
STRAIN=cv. Nipponbare;
TISSUE=Seedling;
Morita S., Kaminaka H., Yokoi H., Masumura T., Tanaka K.;
"Cloning and characterization of cytosolic ascorbate peroxidase cDNA from rice.";
(er) Plant Gene Register PGR97-012.
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Nipponbare;
DOI=10.1101/gr.3869505; PubMed=16109971 [NCBI, ExPASy, EBI, Israel, Japan]
The rice chromosome 3 sequencing consortium;
Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H.-I., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.;
"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species.";
Genome Res. 15:1284-1291(2005).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Nipponbare;
DOI=10.1038/nature03895; PubMed=16100779 [NCBI, ExPASy, EBI, Israel, Japan]
International rice genome sequencing project (IRGSP);
"The map-based sequence of the rice genome.";
Nature 436:793-800(2005).
[4]
GENOME REANNOTATION.
STRAIN=cv. Nipponbare;
DOI=10.1101/gr.5509507; PubMed=17210932 [NCBI, ExPASy, EBI, Israel, Japan]
The rice annotation project (RAP);
"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana.";
Genome Res. 17:175-183(2007).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Nipponbare;
DOI=10.1371/journal.pbio.0030038; PubMed=15685292 [NCBI, ExPASy, EBI, Israel, Japan]
Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L., Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
"The genomes of Oryza sativa: a history of duplications.";
PLoS Biol. 3:266-281(2005).
[6]
PROTEIN SEQUENCE OF 2-11, AND INDUCTION.
STRAIN=cv. Nipponbare;
TISSUE=Leaf;
DOI=10.1002/1615-9861(200208)2:8<947::AID-PROT947>3.0.CO;2-J; PubMed=12203890 [NCBI, ExPASy, EBI, Israel, Japan]
Agrawal G.K., Rakwal R., Yonekura M., Kubo A., Saji H.;
"Proteome analysis of differentially displayed proteins as a tool for investigating ozone stress in rice (Oryza sativa L.) seedlings.";
Proteomics 2:947-959(2002).
[7]
PROTEIN SEQUENCE OF 2-11; 25-34 AND 107-116, AND SUBCELLULAR LOCATION.
STRAIN=cv. Nipponbare;
TISSUE=Anther, Callus, Panicle, Root, Sheath, and Stem;
DOI=10.1093/nar/gkh020; PubMed=14681440 [NCBI, ExPASy, EBI, Israel, Japan]
Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K.;
"Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003.";
Nucleic Acids Res. 32:D388-D392(2004).
[8]
INDUCTION.
DOI=10.1093/jexbot/52.354.145; PubMed=11181723 [NCBI, ExPASy, EBI, Israel, Japan]
Sato Y., Murakami T., Funatsuki H., Matsuba S., Saruyama H., Tanida M.;
"Heat shock-mediated APX gene expression and protection against chilling injury in rice seedlings.";
J. Exp. Bot. 52:145-151(2001).
[9]
TISSUE SPECIFICITY, AND INDUCTION.
DOI=10.1016/j.gene.2003.08.017; PubMed=14644501 [NCBI, ExPASy, EBI, Israel, Japan]
Agrawal G.K., Jwa N.-S., Iwahashi H., Rakwal R.;
"Importance of ascorbate peroxidases OsAPX1 and OsAPX2 in the rice pathogen response pathways and growth and reproduction revealed by their transcriptional profiling.";
Gene 322:93-103(2003).
[10]
NOMENCLATURE.
DOI=10.1007/s00239-004-2666-z; PubMed=15599508 [NCBI, ExPASy, EBI, Israel, Japan]
Teixeira F.K., Menezes-Benavente L., Margis R., Margis-Pinheiro M.;
"Analysis of the molecular evolutionary history of the ascorbate peroxidase gene family: inferences from the rice genome.";
J. Mol. Evol. 59:761-770(2004).
[11]
FUNCTION, ENZYME REGULATION, AND BIOPHYSICOCHEMICAL PROPERTIES.
DOI=10.1007/s10529-004-6587-0; PubMed=15685422 [NCBI, ExPASy, EBI, Israel, Japan]
Lu Z., Takano T., Liu S.;
"Purification and characterization of two ascorbate peroxidases of rice (Oryza sativa L.) expressed in Escherichia coli.";
Biotechnol. Lett. 27:63-67(2005).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
D45423; BAA08264.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AC134232; AAO17000.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
DP000009; ABF95353.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP008209; BAF11683.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
CM000140; EAZ26516.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T03595; T03595.
RefSeq NP_001049769.1; -.
UniGene Os.12723
3D structure databases
HSSP Q43758; 1OAF. [HSSP ENTRY / PDB]
SMR Q10N21; 2-250.
ModBase Q10N21.
Protein family/group databases
PeroxiBase 1865; OsAPx01.
Organism-specific databases
Gramene P93404; -.
Family and domain databases
InterPro IPR002207; Asc_perxdse.
IPR002016; Haem_peroxidase_pln/fun/bac.
Graphical view of domain structure.
Pfam PF00141; peroxidase; 1.
Pfam graphical view of domain structure.
PRINTS PR00459; ASPEROXIDASE.
PR00458; PEROXIDASE.
PROSITE PS00435; PEROXIDASE_1; 1.
PS00436; PEROXIDASE_2; 1.
PS50873; PEROXIDASE_4; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS Q10N21.
Genome annotation databases
GeneID 4332474; -.
KEGG osa:4332474; -.
Other
ProtoNet Q10N21.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Calcium; Cytoplasm; Direct protein sequencing; Heme; Hydrogen peroxide; Iron; Metal-binding; Oxidoreductase; Peroxidase; Potassium; Stress response.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   250  249     L-ascorbate peroxidase 1, cytosolic. PRO_0000055593
ACT_SITE   42    42        Proton acceptor (By similarity). 
METAL   163   163        Iron (heme axial ligand) (By similarity). 
METAL   164   164        Potassium or calcium (By similarity). 
METAL   180   180        Potassium or calcium (By similarity). 
METAL   182   182        Potassium or calcium (By similarity). 
METAL   187   187        Potassium or calcium (By similarity). 
SITE   38    38  1     Transition state stabilizer (By similarity). 
Sequence information
Length: 250 AA [This is the length of the unprocessed precursor] Molecular weight: 27156 Da [This is the MW of the unprocessed precursor] CRC64: 637D70B191CC92E9 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MAKNYPVVSA EYQEAVEKAR QKLRALIAEK SCAPLMLRLA WHSAGTFDVS SKTGGPFGTM 

        70         80         90        100        110        120 
KTPAELSHAA NAGLDIAVRM LEPIKEEIPT ISYADFYQLA GVVAVEVSGG PAVPFHPGRE 

       130        140        150        160        170        180 
DKPAPPPEGR LPDATKGSDH LRQVFGAQMG LSDQDIVALS GGHTLGRCHK ERSGFEGPWT 

       190        200        210        220        230        240 
RNPLQFDNSY FTELLSGDKE GLLQLPSDKA LLSDPAFRPL VEKYAADEKA FFEDYKEAHL 

       250 
KLSELGFADA 

Q10N21 in FASTA format

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