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UniProtKB/Swiss-Prot entry Q12680


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name GLT1_YEAST
Primary accession number Q12680
Secondary accession number Q12290
Integrated into Swiss-Prot on November 1, 1997
Sequence was last modified on September 5, 2006 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 78)
Name and origin of the protein
Protein name Glutamate synthase [NADH] [Precursor]
Synonyms EC 1.4.1.14
NADH-GOGAT
Gene name
Name: GLT1
OrderedLocusNames: YDL171C
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 96744 / CN36;
DOI=10.1002/(SICI)1097-0061(199610)12:13<1359::AID-YEA3>3.0.CO;2-5; PubMed=8923741 [NCBI, ExPASy, EBI, Israel, Japan]
Filetici P., Martegani M.P., Valenzuela L., Gonzalez A., Ballario P.;
"Sequence of the GLT1 gene from Saccharomyces cerevisiae reveals the domain structure of yeast glutamate synthase.";
Yeast 12:1359-1366(1996).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
PubMed=9169867 [NCBI, ExPASy, EBI, Israel, Japan]
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
Nature 387:75-78(1997).
[3]
PROTEIN SEQUENCE OF 54-61, AND SUBUNIT.
STRAIN=ATCC 96744 / CN36;
PubMed=7836314 [NCBI, ExPASy, EBI, Israel, Japan]
Cogoni C., Valenzuela L., Gonzalez-Halphen D., Olivera H., Macino G., Ballario P., Gonzalez A.;
"Saccharomyces cerevisiae has a single glutamate synthase gene coding for a plant-like high-molecular-weight polypeptide.";
J. Bacteriol. 177:792-798(1995).
[4]
FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND SUBSTRATE SPECIFICITY.
PubMed=4362465 [NCBI, ExPASy, EBI, Israel, Japan]
Roon R.J., Even H.L., Larimore F.;
"Glutamate synthase: properties of the reduced nicotinamide adenine dinucleotide-dependent enzyme from Saccharomyces cerevisiae.";
J. Bacteriol. 118:89-95(1974).
[5]
FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY, AND ENZYME REGULATION.
PubMed=7047525 [NCBI, ExPASy, EBI, Israel, Japan]
Masters D.S. Jr., Meister A.;
"Inhibition of homocysteine sulfonamide of glutamate synthase purified from Saccharomyces cerevisiae.";
J. Biol. Chem. 257:8711-8715(1982).
[6]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X89221; CAA61505.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z67750; CAA91574.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z74219; CAA98745.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S61041; S61041.
RefSeq NP_010110.1; -.
3D structure databases
HSSP P55038; 1LM1. [HSSP ENTRY / PDB]
ModBase Q12680.
Protein-protein interaction databases
DIP DIP:6490N; -.
IntAct Q12680; -.
Enzyme and pathway databases
BioCyc MetaCyc:MON-13146; -.
Organism-specific databases
CYGD YDL171c; -.
SGD S000002330; GLT1.
Yeast-GFP YDL171C.
Gene expression databases
ArrayExpress Q12680; -.
GermOnline YDL171C; Saccharomyces cerevisiae.
Ontologies
GO
GO:0031026; Cellular component: glutamate synthase complex (inferred from physical interaction from SGD).
GO:0005739; Cellular component: mitochondrion (inferred from direct assay from SGD).
GO:0006537; Biological process: glutamate biosynthetic process (inferred from expression pattern from SGD).
QuickGo view.
Family and domain databases
InterPro IPR000759; Adrndx_reductase.
IPR013785; Aldolase_TIM.
IPR013027; FAD_pyr_nucl-diS_OxRdtase.
IPR012285; Fum_reductase_C.
IPR000583; GATase_2.
IPR002932; Glu_synth_centr_C.
IPR006982; Glu_synth_centr_N.
IPR002489; Glu_synthase_C.
IPR006005; Glut_synth_sub1.
IPR012220; GOGAT_euk.
IPR001327; Pyr_OxRdtase_NAD_bd.
Graphical view of domain structure.
Gene3D G3DSA:3.20.20.70; Aldolase_TIM; 2.
G3DSA:1.10.1060.10; Fum_reductase_C; 1.
G3DSA:2.160.20.60; Glu_synthase_C; 1.
Pfam PF00310; GATase_2; 1.
PF04898; Glu_syn_central; 1.
PF01645; Glu_synthase; 1.
PF01493; GXGXG; 1.
PF00070; Pyr_redox; 1.
PF07992; Pyr_redox_2; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000187; GOGAT; 1.
PRINTS PR00419; ADXRDTASE.
PR00368; FADPNR.
TIGRFAMs TIGR01317; GOGAT_sm_gam; 1.
PROSITE PS51278; GATASE_TYPE_2; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS Q12680.
Proteomic databases
PeptideAtlas Q12680; -.
Genome annotation databases
Ensembl YDL171C; Saccharomyces cerevisiae. [Contig view]
GeneID 851383; -.
GenomeReviews Z71256_GR; YDL171C.
KEGG sce:YDL171C; -.
NMPDR fig|4932.3.peg.844; -.
Phylogenomic databases
HOGENOM Q12680; -.
Other
LinkHub Q12680; -.
ProtoNet Q12680.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3Fe-4S; Amino-acid biosynthesis; Coiled coil; Complete proteome; Direct protein sequencing; FAD; Flavoprotein; FMN; Glutamate biosynthesis; Glutamine amidotransferase; Iron; Iron-sulfur; Metal-binding; NAD; Oxidoreductase; Zymogen.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
PROPEP   1     53  53      PRO_0000011612
CHAIN   54   2145  2092     Glutamate synthase [NADH]. PRO_0000011613
DOMAIN   54    455  402     Glutamine amidotransferase type-2. 
NP_BIND   1132   1189  58     FMN (By similarity). 
NP_BIND   1928   1942  15     NAD (Potential). 
COILED   1551   1600  50     Potential. 
ACT_SITE   54     54        For GATase activity (By similarity). 
METAL   1185   1185        Iron-sulfur (3Fe-4S) (By similarity). 
METAL   1191   1191        Iron-sulfur (3Fe-4S) (By similarity). 
METAL   1196   1196        Iron-sulfur (3Fe-4S) (By similarity). 
CONFLICT   166    173        NVPVDSTI -> TSRRFYY (in Ref. 1; CAA61505). 
CONFLICT   450    452        FLV -> IPS (in Ref. 1; CAA61505). 
CONFLICT   1753   1753        V -> L (in Ref. 1; CAA61505). 
Sequence information
Length: 2145 AA [This is the length of the unprocessed precursor] Molecular weight: 238102 Da [This is the MW of the unprocessed precursor] CRC64: 78184877D2167A0D [This is a checksum on the sequence]
        10         20         30         40         50         60 
MPVLKSDNFD PLEEAYEGGT IQNYNDEHHL HKSWANVIPD KRGLYDPDYE HDACGVGFVA 

        70         80         90        100        110        120 
NKHGEQSHKI VTDARYLLVN MTHRGAVSSD GNGDGAGILL GIPHEFMKRE FKLDLDLDIP 

       130        140        150        160        170        180 
EMGKYAVGNV FFKKNEKNNK KNLIKCQKIF EDLAASFNLS VLGWRNVPVD STILGDVALS 

       190        200        210        220        230        240 
REPTILQPLL VPLYDEKQPE FNETKFRTQL YLLRKEASLQ IGLENWFYVC SLNNTTIVYK 

       250        260        270        280        290        300 
GQLTPAQVYN YYPDLTNAHF KSHMALVHSR FSTNTFPSWD RAQPLRWLAH NGEINTLRGN 

       310        320        330        340        350        360 
KNWMRSREGV MNSATFKDEL DKLYPIIEEG GSDSAALDNV LELLTINGTL SLPEAVMMMV 

       370        380        390        400        410        420 
PEAYHKDMDS DLKAWYDWAA CLMEPWDGPA LLTFTDGRYC GAILDRNGLR PCRYYITSDD 

       430        440        450        460        470        480 
RVICASEVGV IPIENSLVVQ KGKLKPGDLF LVDTQLGEMV DTKKLKSQIS KRQDFKSWLS 

       490        500        510        520        530        540 
KVIKLDDLLS KTANLVPKEF ISQDSLSLKV QSDPRLLANG YTFEQVTFLL TPMALTGKEA 

       550        560        570        580        590        600 
LGSMGNDAPL ACLNENPVLL YDYFRQLFAQ VTNPPIDPIR EANVMSLECY VGPQGNLLEM 

       610        620        630        640        650        660 
HSSQCDRLLL KSPILHWNEF QALKNIEAAY PSWSVAEIDI TFDKSEGLLG YTDTIDKITK 

       670        680        690        700        710        720 
LASEAIDDGK KILIITDRKM GANRVSISSL IAISCIHHHL IRNKQRSQVA LILETGEARE 

       730        740        750        760        770        780 
IHHFCVLLGY GCDGVYPYLA METLVRMNRE GLLRNVNNDN DTLEEGQILE NYKHAIDAGI 

       790        800        810        820        830        840 
LKVMSKMGIS TLASYKGAQI FEALGLDNSI VDLCFTGTSS RIRGVTFEYL AQDAFSLHER 

       850        860        870        880        890        900 
GYPSRQTISK SVNLPESGEY HFRDGGYKHV NEPTAIASLQ DTVRNKNDVS WQLYVKKEME 

       910        920        930        940        950        960 
AIRDCTLRGL LELDFENSVS IPLEQVEPWT EIARRFASGA MSYGSISMEA HSTLAIAMNR 

       970        980        990       1000       1010       1020 
LGAKSNCGEG GEDAERSAVQ ENGDTMRSAI KQVASARFGV TSYYLSDADE IQIKIAQGAK 

      1030       1040       1050       1060       1070       1080 
PGEGGELPAH KVSKDIAKTR HSTPNVGLIS PPPHHDIYSI EDLKQLIYDL KCANPRAGIS 

      1090       1100       1110       1120       1130       1140 
VKLVSEVGVG IVASGVAKAK ADHILVSGHD GGTGAARWTS VKYAGLPWEL GLAETHQTLV 

      1150       1160       1170       1180       1190       1200 
LNDLRRNVVV QTDGQLRTGF DIAVAVLLGA ESFTLATVPL IAMGCVMLRR CHLNSCAVGI 

      1210       1220       1230       1240       1250       1260 
ATQDPYLRSK FKGQPEHVIN FFYYLIQDLR QIMAKLGFRT IDEMVGHSEK LKKRDDVNAK 

      1270       1280       1290       1300       1310       1320 
AINIDLSPIL TPAHVIRPGV PTKFTKKQDH KLHTRLDNKL IDEAEVTLDR GLPVNIDASI 

      1330       1340       1350       1360       1370       1380 
INTDRALGST LSYRVSKKFG EDGLPKDTVV VNIEGSAGQS FGAFLASGIT FILNGDANDY 

      1390       1400       1410       1420       1430       1440 
VGKGLSGGII VIKPPKDSKF KSDENVIVGN TCFYGATSGT AFISGSAGER FGVRNSGATI 

      1450       1460       1470       1480       1490       1500 
VVERIKGNNA FEYMTGGRAI VLSQMESLNA FSGATGGIAY CLTSDYDDFV GKINKDTVEL 

      1510       1520       1530       1540       1550       1560 
ESLCDPVEIA FVKNLIQEHW NYTQSDLAAR ILGNFNHYLK DFVKVIPTDY KKVLLKEKAE 

      1570       1580       1590       1600       1610       1620 
AAKAKAKATS EYLKKFRSNQ EVDDEVNTLL IANQKAKEQE KKKSITISNK ATLKEPKVVD 

      1630       1640       1650       1660       1670       1680 
LEDAVPDSKQ LEKNSERIEK TRGFMIHKRR HETHRDPRTR VNDWKEFTNP ITKKDAKYQT 

      1690       1700       1710       1720       1730       1740 
ARCMDCGTPF CLSDTGCPLS NIIPKFNELL FKNQWKLALD KLLETNNFPE FTGRVCPAPC 

      1750       1760       1770       1780       1790       1800 
EGACTLGIIE DPVGIKSVER IIIDNAFKEG WIKPCPPSTR TGFTVGVIGS GPAGLACADM 

      1810       1820       1830       1840       1850       1860 
LNRAGHTVTV YERSDRCGGL LMYGIPNMKL DKAIVQRRID LLSAEGIDFV TNTEIGKTIS 

      1870       1880       1890       1900       1910       1920 
MDELKNKHNA VVYAIGSTIP RDLPIKGREL KNIDFAMQLL ESNTKALLNK DLEIIREKIQ 

      1930       1940       1950       1960       1970       1980 
GKKVIVVGGG DTGNDCLGTS VRHGAASVLN FELLPEPPVE RAKDNPWPQW PRVMRVDYGH 

      1990       2000       2010       2020       2030       2040 
AEVKEHYGRD PREYCILSKE FIGNDEGEVT AIRTVRVEWK KSQSGVWQMV EIPNSEEIFE 

      2050       2060       2070       2080       2090       2100 
ADIILLSMGF VGPELINGND NEVKKTRRGT IATLDDSSYS IDGGKTFACG DCRRGQSLIV 

      2110       2120       2130       2140 
WAIQEGRKCA ASVDKFLMDG TTYLPSNGGI VQRDYKLLKE LASQV 

Q12680 in FASTA format

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