FUNCTION: Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate (By similarity).
CATALYTIC ACTIVITY: 5,10-methylenetetrahydrofolate + NADP⁺ = 5,10-methenyltetrahydrofolate + NADPH.
CATALYTIC ACTIVITY: 5,10-methenyltetrahydrofolate + H₂O = 10-formyltetrahydrofolate.
PATHWAY: One-carbon metabolism; tetrahydrofolate pathway .
SUBUNIT: Homodimer (By similarity).
SIMILARITY: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000362; ABG33672.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_684358.1; -.

3D structure databases

ModBase

Q160C1.

Enzyme and pathway databases

BioCyc

RDEN375451:RD1_4236-MON; -.

Ontologies

GO:0004477;	Molecular function: methenyltetrahydrofolate cyclohydrolase activity (inferred from electronic annotation from HAMAP).
GO:0004488;	Molecular function: methylenetetrahydrofolate dehydrogenase (NADP+) activity (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01576; -; 1.
PBIL [Tree]

InterPro

IPR016040; NAD(P)-bd.
IPR000672; THF_DHase/CycOHase.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

Pfam

PF00763; THF_DHG_CYH; 1.
PF02882; THF_DHG_CYH_C; 1.
Pfam graphical view of domain structure.

PRINTS

PR00085; THFDHDRGNASE.

ProDom

PD002300; THFDhg/Cyc_hydro; 1.
[Domain structure / List of seq. sharing at least 1 domain]

PROSITE

PS00766; THF_DHG_CYH_1; 1.
PS00767; THF_DHG_CYH_2; 1.

BLOCKS

Q160C1.

Genome annotation databases

GeneID

4195554; -.

GenomeReviews

CP000362_GR; RD1_4236.

KEGG

rde:RD1_4236; -.

NMPDR

fig|375451.6.peg.3934; -.

Phylogenomic databases

HOGENOM

Q160C1; -.

Genome annotation databases

CMR

Q160C1; RD1_4236.

Other

ProtoNet

Q160C1.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Amino-acid biosynthesis; Complete proteome; Histidine biosynthesis; Hydrolase; Methionine biosynthesis; Multifunctional enzyme; NADP; One-carbon metabolism; Oxidoreductase; Purine biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 300`	`300`		`Bifunctional protein folD 2.`	`PRO_0000268483`

Sequence information

Length: 300 AA [This is the length of the unprocessed precursor]

Molecular weight: 31422 Da [This is the MW of the unprocessed precursor]

CRC64: 3FE1088477B13EA0 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAATIIDGKA FAAKVRAQVA EHVTRIKQDH GITPGLAVVL VGEDPASQVY VRSKGKQTVE 

        70         80         90        100        110        120 
AGMNSYEHKL DADTSEADLL AVVDKLNKDP NVHGILVQLP LPKHLDEDLI INSIDPAKDV 

       130        140        150        160        170        180 
DGFHISNVGL LGTGQKSMVP CTPLGCLMML REHHGSLSGM NAVVIGRSNI VGKPMAQLLL 

       190        200        210        220        230        240 
NDSCTVTIAH SRTKDLPDVV RGADIVVAAV GRPEMVPGDW IKPGATVIDV GINRIDAPEK 

       250        260        270        280        290        300 
GEGKTRLVGD CHYDSCAETA GAITPVPGGV GPMTIACLLA NTVTSCCRAN GLEEPTGLTA

Q160C1 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools