FUNCTION: Flavoprotein monooxygenase required for N-hydroxylation of the two acylated lysine residues during mycobactin assembly, thus producing the hydroxamate groups necessary for iron sequestration. Is also able, but less efficiently, to hydroxylate L-lysine (non acylated) in vitro (By similarity).
CATALYTIC ACTIVITY: L-lysine + NADPH + O₂ = N⁶-hydroxy-L-lysine + NADP⁺ + H₂O.
COFACTOR: FAD (By similarity).
PATHWAY: Siderophore biosynthesis; mycobactin biosynthesis .
SIMILARITY: Belongs to the lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family.

Copyright

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Cross-references

Sequence databases

EMBL

CP000384; ABG09571.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_640627.1; -.

3D structure databases

ModBase

Q1B6B3.

Enzyme and pathway databases

BioCyc

MSP164756:MMCS_3464-MON; -.

Ontologies

GO:0047091;	Molecular function: L-lysine 6-monooxygenase (NADPH) activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

BLOCKS

Q1B6B3.

Genome annotation databases

GeneID

4112296; -.

GenomeReviews

CP000384_GR; Mmcs_3464.

KEGG

mmc:Mmcs_3464; -.

Phylogenomic databases

HOGENOM

Q1B6B3; -.

Genome annotation databases

CMR

Q1B6B3; Mmcs_3464.

Other

ProtoNet

Q1B6B3.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; FAD; Flavoprotein; Ion transport; Iron; Iron transport; Monooxygenase; NADP; Oxidoreductase; Signal; Transport.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 21`	`21`	`Potential.`
`CHAIN`	`22 430`	`409`	`L-lysine 6-monooxygenase mbtG.`	`PRO_0000261311`

Sequence information

Length: 430 AA [This is the length of the unprocessed precursor]

Molecular weight: 46622 Da [This is the MW of the unprocessed precursor]

CRC64: 2F943638F21DD3A2 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTATLAVIGA GPKAVAVAAK AAELRNMGVD APDVVVVERA GVGANWTAAG GWTDGQHRLG 

        70         80         90        100        110        120 
TSPEKDIGFP YRSSLVPRRN AELDDRMTRH SWQAYLVATS QFAEWIDRGR PAPNHHRWAA 

       130        140        150        160        170        180 
YLRWVADAIG MNVVHGEVER ISIGGRGWEL HTPESTVAAD AVMITGPGQA ERTLLPGHPR 

       190        200        210        220        230        240 
VMSIADFWRR TAGHELIVAE RVAMIGGGET AASMLNELFR HRVSTITVIS PQVTLFTRGE 

       250        260        270        280        290        300 
GFFENTLFSD PTGWTSLTLA ERRDAMFRTD RGVFSARVQE ALLADDRIRH LRGRVAHAVP 

       310        320        330        340        350        360 
RDGRIRLTLH TDRAGERVET VHGFDLVIDG QGADALWFLP LLGQDARDLL ELGLGGPLTG 

       370        380        390        400        410        420 
ELLQESIGHD LAVGGVTPKL FLPGLAGLNQ GPGFPNLSCL GLMSDRILGA DLGANAAMTN 

       430 
RRSIEHQPIR

Q1B6B3 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools