NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1371/journal.pbio.0040188; PubMed=16729848 [NCBI, ExPASy, EBI, Israel, Japan]
Wu D., Daugherty S.C., Van Aken S.E., Pai G.H., Watkins K.L., Khouri H., Tallon L.J., Zaborsky J.M., Dunbar H.E., Tran P.L., Moran N.A., Eisen J.A.;
"Metabolic complementarity and genomics of the dual bacterial symbiosis of sharpshooters.";
PLoS Biol. 4:1079-1092(2006).

Comments

FUNCTION: This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate (By similarity).
CATALYTIC ACTIVITY: H₂S + 3 NADP⁺ + 3 H₂O = sulfite + 3 NADPH.
COFACTOR: Binds 1 siroheme per subunit (By similarity).
COFACTOR: Binds 1 4Fe-4S cluster per subunit (By similarity).
SUBUNIT: Alpha(8)-beta(4). The alpha component is a flavoprotein, the beta component is a hemoprotein (By similarity).
SIMILARITY: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000238; ABF13903.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_588672.1; -.

3D structure databases

ModBase

Q1LTP2.

Ontologies

GO:0004783;	Molecular function: sulfite reductase (NADPH) activity (inferred from electronic annotation from HAMAP).
GO:0000103;	Biological process: sulfate assimilation (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01540; -; 1.
PBIL [Tree]

InterPro

IPR011786; CysI.
IPR006066; Nir_Si_BS.
IPR006067; Nir_Sir_4Fe4S.
IPR005117; NiRdtase/SiRdtase_haem-b_fer.
Graphical view of domain structure.

Pfam

PF01077; NIR_SIR; 1.
PF03460; NIR_SIR_ferr; 2.
Pfam graphical view of domain structure.

PR00397; SIROHAEM.

TIGR02041; CysI; 1.

PS00365; NIR_SIR; 1.

Genome annotation databases

GeneID

4056488; -.

GenomeReviews

CP000238_GR; BCI_0217.

KEGG

bci:BCI_0217; -.

Phylogenomic databases

HOGENOM

Q1LTP2; -.

Genome annotation databases

CMR

Q1LTP2; BCI_0217.

Other

ProtoNet

Q1LTP2.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

4Fe-4S; Amino-acid biosynthesis; Complete proteome; Cysteine biosynthesis; Heme; Iron; Iron-sulfur; Metal-binding; NADP; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 566`	`566`	`Sulfite reductase [NADPH] hemoprotein beta-component.`	`PRO_0000292958`
`METAL`	`430 430`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`436 436`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`475 475`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`479 479`		`Iron (siroheme axial ligand) (By similarity).`
`METAL`	`479 479`		`Iron-sulfur (4Fe-4S) (By similarity).`

Sequence information

Length: 566 AA [This is the length of the unprocessed precursor]

Molecular weight: 63931 Da [This is the MW of the unprocessed precursor]

CRC64: 241F9CAD6D6E7ED4 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MNKKIPKAIL SDNERLKVAS NFLRGTIAQD LQDNITGGFK GDNIQLIRFH GMYQQDDRDL 

        70         80         90        100        110        120 
RVERTCQKLE PLINMMLRCR LPGGIITPQQ WLGIDSFATK HTLYGSIRLT TRQTFQFHGV 

       130        140        150        160        170        180 
LKPNLKNMHK LLHSLGLDSI ATAGDMNRNV LCTSNPVESV LHQQVCNYAK MISEHFLPKT 

       190        200        210        220        230        240 
RAYAEIWLDG EKTETTEQEP ILGANYLPRK FKISIVVPPL NDVDLHANDL NFIAISNLGQ 

       250        260        270        280        290        300 
LVGFNVLVGG GLAMTHNDKS TYPRTASELG YISVVDTIKI AEAVITTQRD LGDRSNRKHA 

       310        320        330        340        350        360 
KTKYTIERVG VEFFKKEVEV RAGIKFKHIR PYSFTERGDR FGWVQGIDNQ WHLTLFIENG 

       370        380        390        400        410        420 
RIIDDSHRKL KTGMLEIARI HQGDFRITAN QNIIIAGVEK KHKATIELLA RQYGLINNNI 

       430        440        450        460        470        480 
TLQRKASMAC VAFPTCPLAM AEAERFLPQF VTKVENIMSR HGLGQEKIIL RVTGCPNGCG 

       490        500        510        520        530        540 
RAMLAEIGLV GKTIGRYNLY LGGDSIGTRI PRIYRENLTE EEILSIINDT TGRWARERQP 

       550        560 
DESYGDYVVR AGIIRPVINS ALDFHN

Q1LTP2 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools