NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Prostate;
DOI=10.1016/0960-0760(94)00183-M; PubMed=7734398 [NCBI, ExPASy, EBI, Israel, Japan]
Levy M.A., Brandt M., Sheedy K.M., Holt D.A., Heaslip J.I., Trill J.J., Ryan P.J., Morris R.A., Garrison L.M., Bergsma D.J.;
"Cloning, expression and functional characterization of type 1 and type 2 steroid 5 alpha-reductases from Cynomolgus monkey: comparisons with human and rat isoenzymes.";
J. Steroid Biochem. Mol. Biol. 52:307-319(1995).

Comments

FUNCTION: Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
CATALYTIC ACTIVITY: A 3-oxo-5-alpha-steroid + acceptor = a 3-oxo-Delta⁴-steroid + reduced acceptor.
BIOPHYSICOCHEMICAL PROPERTIES:

pH dependence: Optimally active at alkaline pHs;
SUBCELLULAR LOCATION: Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein (Potential).
SIMILARITY: Belongs to the steroid 5-alpha reductase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

S77162; AAB34212.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q28891.

Family and domain databases

InterPro

IPR016636; 3-oxo-5-alpha-steroid_4-DH.
IPR001104; 3-oxo-5_a-steroid_4-DHase_C.
Graphical view of domain structure.

Pfam

PF02544; Steroid_dh; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF015596; 5_alpha-SR2; 1.

PROSITE

PS50244; S5A_REDUCTASE; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q28891.

Phylogenomic databases

HOVERGEN

Q28891; -.

Other

ProtoNet

Q28891.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Differentiation; Endoplasmic reticulum; Membrane; Microsome; Oxidoreductase; Sexual differentiation; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 263`	`263`	`3-oxo-5-alpha-steroid 4-dehydrogenase 1.`	`PRO_0000213675`
`TRANSMEM`	`16 33`	`18`	`Potential.`
`TRANSMEM`	`90 110`	`21`	`Potential.`
`TRANSMEM`	`115 135`	`21`	`Potential.`
`TRANSMEM`	`155 175`	`21`	`Potential.`
`TRANSMEM`	`213 233`	`21`	`Potential.`

Sequence information

Length: 263 AA [This is the length of the unprocessed precursor]

Molecular weight: 30018 Da [This is the MW of the unprocessed precursor]

CRC64: 8830E1FB8F7365C4 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MATAVAEELL LAEERMLAAL AYLQCAVGCA VLARNRETNL AYGRHASPSF RVRVPARAAW 

        70         80         90        100        110        120 
VVQELPSLAL PLYQYASESA PRLRSAPNCI LLAMFLVHYG HRCLIYPFLM RGGKPMPLLA 

       130        140        150        160        170        180 
CTMAIMFCTF NGYLQSRYLS HWAVYADDWV TDPRFLIGFG LWLAGMLINI HSDHILRNLR 

       190        200        210        220        230        240 
KPGDTGYKIP RGGLFEYVTA ANYFGEIMEW CGYALASWSV QGAAFAFFTF CFLSGRAKEH 

       250        260 
HRWYLQKFEE YPKFRKILIP FLF

Q28891 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools