NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Prostate;
DOI=10.1016/0960-0760(94)00183-M; PubMed=7734398 [NCBI, ExPASy, EBI, Israel, Japan]
Levy M.A., Brandt M., Sheedy K.M., Holt D.A., Heaslip J.I., Trill J.J., Ryan P.J., Morris R.A., Garrison L.M., Bergsma D.J.;
"Cloning, expression and functional characterization of type 1 and type 2 steroid 5 alpha-reductases from Cynomolgus monkey: comparisons with human and rat isoenzymes.";
J. Steroid Biochem. Mol. Biol. 52:307-319(1995).

Comments

FUNCTION: Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
CATALYTIC ACTIVITY: A 3-oxo-5-alpha-steroid + acceptor = a 3-oxo-Delta⁴-steroid + reduced acceptor.
BIOPHYSICOCHEMICAL PROPERTIES:

pH dependence: Optimally active at acidic pHs;
SUBCELLULAR LOCATION: Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein (Potential).
SIMILARITY: Belongs to the steroid 5-alpha reductase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

S77165; AAB34213.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q28892.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.

Family and domain databases

InterPro

IPR016636; 3-oxo-5-alpha-steroid_4-DH.
IPR001104; 3-oxo-5_a-steroid_4-DHase_C.
IPR002110; ANK.
Graphical view of domain structure.

Pfam

PF02544; Steroid_dh; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF015596; 5_alpha-SR2; 1.

PRINTS

PR01415; ANKYRIN.

PROSITE

PS50244; S5A_REDUCTASE; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q28892.

Phylogenomic databases

HOVERGEN

Q28892; -.

Other

ProtoNet

Q28892.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Differentiation; Endoplasmic reticulum; Membrane; Microsome; Oxidoreductase; Sexual differentiation; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 254`	`254`	`3-oxo-5-alpha-steroid 4-dehydrogenase 2.`	`PRO_0000213677`
`TRANSMEM`	`8 28`	`21`	`Potential.`
`TRANSMEM`	`72 92`	`21`	`Potential.`
`TRANSMEM`	`146 166`	`21`	`Potential.`
`TRANSMEM`	`206 226`	`21`	`Potential.`

Sequence information

Length: 254 AA [This is the length of the unprocessed precursor]

Molecular weight: 28504 Da [This is the MW of the unprocessed precursor]

CRC64: 0699C2E07C77653B [This is a checksum on the sequence]

        10         20         30         40         50         60 
MQVQCQQSPV LAGSATLVAL GALVLYVAKP SGYGKHTESL KPAATRLPAR AAWFLQELPS 

        70         80         90        100        110        120 
FAVPAGILAR QPLSLFGPPG TVLLGLFCVH YFHRTFVYSL LNRGRPYPAV LIFRGIAFCA 

       130        140        150        160        170        180 
GNGFLQSYYL IYCAEYPDGW YTDIRFCLGV FLFILGMGVN IHSDYILRQL RKPGEITYRI 

       190        200        210        220        230        240 
PKGGLFTYVS GANFLGEIIE WIGYALATWS LPALAFAFFS VCFLGLRAFH HHRFYLKMFE 

       250 
DYPKSRKALI PFIF

Q28892 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools