NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Nipponbare;
DOI=10.1186/1741-7007-3-20; PubMed=16188032 [NCBI, ExPASy, EBI, Israel, Japan]
The rice chromosomes 11 and 12 sequencing consortia;
"The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications.";
BMC Biol. 3:20-20(2005).

Comments

CATALYTIC ACTIVITY: 5,6,7,8-tetrahydrofolate + NADP⁺ = 7,8-dihydrofolate + NADPH.
CATALYTIC ACTIVITY: 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.
PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis; tetrahydrofolate from dihydrofolate: step 1/1 .
MISCELLANEOUS: The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.
SIMILARITY: In the N-terminal section; belongs to the dihydrofolate reductase family.
SIMILARITY: In the C-terminal section; belongs to the thymidylate synthase family.
SIMILARITY: Contains 1 DHFR (dihydrofolate reductase) domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

DP000011; ABA98037.2; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q2QRX6.

Family and domain databases

InterPro

IPR012262; DHFR-TS.
IPR001796; DHFR_reg.
IPR000398; Thymidylat_synth_C.
Graphical view of domain structure.

Gene3D

G3DSA:3.30.572.10; Thymidylat_synth_C; 1.

PANTHER

PTHR11549:SF2; Thymidylat_synth_C; 1.

Pfam

PF00186; DHFR_1; 1.
PF00303; Thymidylat_synt; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000389; DHFR-TS; 1.

PRINTS

PR00070; DHFR.
PR00108; THYMDSNTHASE.

ProDom

PD001180; Thymidylat_synth; 1.
[Domain structure / List of seq. sharing at least 1 domain]

TIGRFAMs

TIGR03284; thym_sym; 1.

PROSITE

PS00075; DHFR_1; 1.
PS51330; DHFR_2; 1.
PS00091; THYMIDYLATE_SYNTHASE; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Methyltransferase; Multifunctional enzyme; NADP; Nucleotide biosynthesis; One-carbon metabolism; Oxidoreductase; Transferase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 494`	`494`	`Putative bifunctional dihydrofolate reductase-thymidylate synthase.`	`PRO_0000247485`
`DOMAIN`	`1 167`	`167`	`DHFR.`
`REGION`	`170 494`	`325`	`Thymidylate synthase.`
`ACT_SITE`	`376 376`		`By similarity.`

Sequence information

Length: 494 AA [This is the length of the unprocessed precursor]

Molecular weight: 55977 Da [This is the MW of the unprocessed precursor]

CRC64: 1F7F2D5162109A1B [This is a checksum on the sequence]

        10         20         30         40         50         60 
MGIGKDGTLP WKLPGDLKFF KDITVTTSDP SKKNAVVMGR KTWESIPLKF RPLPGRLNVI 

        70         80         90        100        110        120 
LTRSGSFDFA TAENVVICGS LDSALQLLAT TPYCLTVEKT FIIGGGEILR QSLNAPACEA 

       130        140        150        160        170        180 
IHLTDIESSI ECDTFIPPID LSMFHPWYSS FPVVENGIKH SFISFVRVTK SIAEANDSSG 

       190        200        210        220        230        240 
KELTGNDSKK VKFEIENFSF LPKMIFERHE EYQYLNLVQD IIRNGAKKND RTGTGTVSKF 

       250        260        270        280        290        300 
GCQMRFNLRR NFPLLTTKRV FWRGVLEELL WFISGSTNAK VLQEKGIHIW DGNASRQYLD 

       310        320        330        340        350        360 
SIGLTQREEG DLGPVYGFQW RHFGAEYTDM HADYVGKGFD QLMDVIDKIK NNPDDRRIIL 

       370        380        390        400        410        420 
SAWNPTDLKK MALPPCHMFA QFYVENGELS CQMYQRSADM GLGVPFNIAS YSLLTCMIAQ 

       430        440        450        460        470        480 
VCDLSPGDFV HVIGDAHVYR THVEALEEQM RKQPKPFPIL KINPVKKDID SFVTSDFKLV 

       490 
RYDPHHKIEM KMAV

Q2QRX6 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools