NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P.;
"Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942.";
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) (By similarity).
CATALYTIC ACTIVITY: Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = 13¹-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP⁺ + H₂O.
CATALYTIC ACTIVITY: 13¹-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = 13¹-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP⁺ + 2 H₂O.
CATALYTIC ACTIVITY: 13¹-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = divinylprotochlorophyllide + NADP⁺ + 2 H₂O.
COFACTOR: Iron (By similarity).
PATHWAY: Porphyrin metabolism; chlorophyll biosynthesis (light-independent).
SIMILARITY: Belongs to the acsF family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000100; ABB57937.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_400924.1; -.

3D structure databases

ModBase

Q31LY2.

Enzyme and pathway databases

BioCyc

SELO1140:SYNPCC7942_1907-MON; -.

Ontologies

GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from HAMAP).
GO:0048529;	Molecular function: magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity (inferred from electronic annotation from HAMAP).
GO:0015979;	Biological process: photosynthesis (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01840; -; 1.
PBIL [Tree]

InterPro

IPR008434; AcsF.
IPR003251; Rubrerythrin.
Graphical view of domain structure.

Pfam

PF02915; Rubrerythrin; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR02029; AcsF; 1.

BLOCKS

Q31LY2.

Genome annotation databases

GeneID

3775270; -.

GenomeReviews

CP000100_GR; Synpcc7942_1907.

KEGG

syf:Synpcc7942_1907; -.

Phylogenomic databases

HOGENOM

Q31LY2; -.

Genome annotation databases

CMR

Q31LY2; Synpcc7942_1907.

Other

ProtoNet

Q31LY2.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Chlorophyll biosynthesis; Complete proteome; Iron; Metal-binding; NADP; Oxidoreductase; Photosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 358`	`358`		`Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase.`	`PRO_1000070552`

Sequence information

Length: 358 AA [This is the length of the unprocessed precursor]

Molecular weight: 42120 Da [This is the MW of the unprocessed precursor]

CRC64: 6C047F45E9DAB3A2 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MVDSLQKPEL EELRPGIKAP AKETILTPRF YTTDFEAMAK MDLSPNEDEL RAILEEFRAD 

        70         80         90        100        110        120 
YNRKHFVRTP EFDGSAADMD PETKKVFVEF LEQSCTSEFS GFLLYKELSR RIKNRNPLLA 

       130        140        150        160        170        180 
ECFELMARDE ARHAGFLNKS MSDFDLQLDL GFLTANKKYT YFKPAYIFYA TYLSEKIGYW 

       190        200        210        220        230        240 
RYITIFRHLE QHPEYRIYPI FKFFENWCQD ENRHGDFFDA LMKAQPSTVR GFVAKLWCRF 

       250        260        270        280        290        300 
FLLAVFATMY IRDVQRKEFY EALGLDAREY DRHVIAKTNE TSARVFPVVL DVDHPKFYDR 

       310        320        330        340        350 
LETCIANNNQ LAAIEATSAP KPVKVLRKLP YYLSNGLQLL KLYLVKPLES DVYQPAVR

Q31LY2 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools