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[1]
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NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, AND FUNCTION.
STRAIN=cv. Romaine;
DOI=10.1073/pnas.93.13.6320; PubMed=8692813 [NCBI, ExPASy, EBI, Israel, Japan]
Bugos R.C.,
Yamamoto H.Y.;
"Molecular cloning of violaxanthin de-epoxidase from romaine lettuce and expression in Escherichia coli.";
Proc. Natl. Acad. Sci. U.S.A. 93:6320-6325(1996).
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[2]
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PROTEIN SEQUENCE OF 126-138; 265-272; 275-289 AND 341-353, AND BIOPHYSICOCHEMICAL PROPERTIES.
STRAIN=cv. Romaine;
DOI=10.1104/pp.110.2.697; PubMed=8742341 [NCBI, ExPASy, EBI, Israel, Japan]
Rockholm D.C.,
Yamamoto H.Y.;
"Violaxanthin de-epoxidase.";
Plant Physiol. 110:697-703(1996).
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- FUNCTION: Part of the xanthophyll (or violaxanthin) cycle for controlling the concentration of zeaxanthin in chloroplasts. Catalyzes the two-step mono de-epoxidation reaction. Stereospecific for all-trans xanthophylls. Zeaxanthin induces the dissipation of excitation energy in the chlorophyll of the light-harvesting protein complex of photosystem II.
- CATALYTIC ACTIVITY: Violaxanthin + ascorbate = antheraxanthin + dehydroascorbate + H2O.
- CATALYTIC ACTIVITY: Antheraxanthin + ascorbate = zeaxanthin + dehydroascorbate + H2O.
- ENZYME REGULATION: Inhibited by DTT.
- BIOPHYSICOCHEMICAL PROPERTIES:
| Kinetic parameters: |
KM=0.352 µM for violaxanthin; | | KM=4.4 mM for ascorbate; | | Note=reaction significantly slower in the absence of monogalactosyldiacylglyceride; | | pH dependence: |
Optimum pH is 5.0; | |
- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane; Peripheral membrane protein; Lumenal side (By similarity).
- DEVELOPMENTAL STAGE: Low constitutive expression in etiolated seedlings and immature yellow leaves. Higher expression in mature green leaves.
- MISCELLANEOUS: Associates specificaly at acidic pH with monogalactosyldiacylglyceride (MGDG), the major lipid of thylakoids.
- SIMILARITY: Belongs to the calycin superfamily. Lipocalin family.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 473 AA [This is the length of the unprocessed precursor] |
Molecular weight: 54448 Da [This is the MW of the unprocessed precursor] |
CRC64: 1B22522DC2C62699 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MALSLHTVFL CKEEALNLYA RSPCNERFHR SGQPPTNIIM MKIRSNNGYF NSFRLFTSYK
70 80 90 100 110 120
TSSFSDSSHC KDKSQICSID TSFEEIQRFD LKRGMTLILE KQWRQFIQLA IVLVCTFVIV
130 140 150 160 170 180
PRVDAVDALK TCACLLKECR IELAKCIANP SCAANVACLQ TCNNRPDETE CQIKCGDLFE
190 200 210 220 230 240
NSVVDQFNEC AVSRKKCVPR KSDVGEFPVP DRNAVVQNFN MKDFSGKWYI TSGLNPTFDA
250 260 270 280 290 300
FDCQLHEFHM ENDKLVGNLT WRIKTLDGGF FTRSAVQTFV QDPDLPGALY NHDNEFLHYQ
310 320 330 340 350 360
DDWYILSSQI ENKPDDYIFV YYRGRNDAWD GYGGSVIYTR SPTLPESIIP NLQKAAKSVG
370 380 390 400 410 420
RDFNNFITTD NSCGPEPPLV ERLEKTAEEG EKLLIKEAVE IEEEVEKEVE KVRDTEMTLF
430 440 450 460 470
QRLLEGFKEL QQDEENFVRE LSKEEKEILN ELQMEATEVE KLFGRALPIR KLR
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Q40251 in FASTA format |
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