NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Desiree;
TISSUE=Leaf;
PubMed=9615461 [NCBI, ExPASy, EBI, Israel, Japan]
Rasmusson A.G., Heiser V., Irrgang K.D., Brennicke A., Grohmann L.;
"Molecular characterisation of the 76 kDa iron-sulphur protein subunit of potato mitochondrial complex I.";
Plant Cell Physiol. 39:373-381(1998).

Comments

FUNCTION: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity).
CATALYTIC ACTIVITY: NADH + ubiquinone = NAD⁺ + ubiquinol.
CATALYTIC ACTIVITY: NADH + acceptor = NAD⁺ + reduced acceptor.
COFACTOR: Binds 1 2Fe-2S cluster per subunit (By similarity).
COFACTOR: Binds 2 4Fe-4S clusters per subunit (By similarity).
SUBUNIT: Complex I is composed of about 30 different subunits (By similarity).
SUBCELLULAR LOCATION: Mitochondrion inner membrane (By similarity). Note=Matrix and cytoplasmic side of the mitochondrial inner membrane (By similarity).
SIMILARITY: Belongs to the complex I 75 kDa subunit family.
SIMILARITY: Contains 1 2Fe-2S ferredoxin-type domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X85808; CAA59818.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S52737; S52737.

3D structure databases

ModBase

Q43644.

Family and domain databases

InterPro

IPR006058; 2Fe2S_fd_BS.
IPR001041; Ferredoxin.
IPR006656; Mopterin_OxRdtase.
IPR000283; NADH_DHase_75KDa_su_CS.
IPR010228; NADH_quinone_OxRdtase_G.
IPR015405; NuoG_C.
Graphical view of domain structure.

Pfam

PF09326; DUF1982; 1.
PF00111; Fer2; 1.
PF00384; Molybdopterin; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR01973; NuoG; 1.

PROSITE

PS00197; 2FE2S_FER_1; FALSE_NEG.
PS51085; 2FE2S_FER_2; 1.
PS00641; COMPLEX1_75K_1; 1.
PS00642; COMPLEX1_75K_2; 1.
PS00643; COMPLEX1_75K_3; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

2Fe-2S; 4Fe-4S; Electron transport; Iron; Iron-sulfur; Membrane; Metal-binding; Mitochondrion; Mitochondrion inner membrane; NAD; Oxidoreductase; Respiratory chain; Transit peptide; Transport; Ubiquinone.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 27`	`27`	`Mitochondrion (By similarity).`
`CHAIN`	`28 738`	`711`	`NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial.`	`PRO_0000019973`
`DOMAIN`	`66 144`	`79`	`2Fe-2S ferredoxin-type.`
`METAL`	`100 100`		`Iron-sulfur 1 (2Fe-2S) (By similarity).`
`METAL`	`111 111`		`Iron-sulfur 1 (2Fe-2S) (By similarity).`
`METAL`	`114 114`		`Iron-sulfur 1 (2Fe-2S) (By similarity).`
`METAL`	`128 128`		`Iron-sulfur 1 (2Fe-2S) (By similarity).`
`METAL`	`160 160`		`Iron-sulfur 2 (4Fe-4S); via pros nitrogen (By similarity).`
`METAL`	`164 164`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`167 167`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`173 173`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`212 212`		`Iron-sulfur 3 (4Fe-4S) (By similarity).`
`METAL`	`215 215`		`Iron-sulfur 3 (4Fe-4S) (By similarity).`
`METAL`	`218 218`		`Iron-sulfur 3 (4Fe-4S) (By similarity).`
`METAL`	`262 262`		`Iron-sulfur 3 (4Fe-4S) (By similarity).`

Sequence information

Length: 738 AA [This is the length of the unprocessed precursor]

Molecular weight: 79970 Da [This is the MW of the unprocessed precursor]

CRC64: 4AA955A62EEAD2E8 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MGLGLLASRA LRSSRIIRNS TRTIVSTPEL KNADAAAAAA AADAPSDLPK RHPVGGARVH 

        70         80         90        100        110        120 
LPNPEDVIEV FVDGYPVKIP KGMTVLQACE IAGVDIPRFC YHSRLSIAGN CRMCLVEVEK 

       130        140        150        160        170        180 
SPKPVASCAM PALPGMKIKT DTPIAKKARE GVMEFLLMNH PLDCPICDQG GECDLQDQSM 

       190        200        210        220        230        240 
AFGSDRGRFT EMKRSVVDKN LGPLVKTVMT RCIQCTRCVR FASEVAGVED LGMLGRGSGE 

       250        260        270        280        290        300 
EIGTYVEKLM TSELSGNVID ICPVGALTSK PFAFKARNWE LKGTESIDVT DAVGSNIRID 

       310        320        330        340        350        360 
SRGPEVMRVV PRLNEDINEE WISDKTRFFY DGLKRQRLND PMIRGADGRF QAVSWRDALA 

       370        380        390        400        410        420 
IVAEVMHQIK PEEIVGVAGK LSDAESMMAL KDLLNKMGSN NIFCEGNGMH PNADLRSGYI 

       430        440        450        460        470        480 
MNTSISGLEK ADAFLLVGTQ PRVEAAMVNA RIHKTVKATN AKVGYVGPAA DFNYDHEHLG 

       490        500        510        520        530        540 
TDPQTLVEIA EGRHPFSSAL KNAKNPVIIV GAGVFDRDDK DAVFAAVDTI AKNNNVVRPD 

       550        560        570        580        590        600 
WNGLNVLLLN AAQVAALDLG LVPESDKCIE SAKFVYLMGA DDVNLDKLPD DAFVVYQGHH 

       610        620        630        640        650        660 
GDRGVYRANV ILPASAFTEK EGIYENTEGC AQITLPAVPT VGDARDDWKI VRALSEVAGV 

       670        680        690        700        710        720 
GLPYDSLGAI RSRIKTVAPN LLEVDERQPA TFSTSLRPEV SQKVSATPFT PAVENFYMTD 

       730 
AITRASKIMA QCSALLKK

Q43644 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools