NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Desiree;
TISSUE=Leaf;
DOI=10.1007/BF00040836; PubMed=8914535 [NCBI, ExPASy, EBI, Israel, Japan]
Heiser V., Grohmann L., Brennicke A.;
"The plant mitochondrial 22 kDa (PSST) subunit of respiratory chain complex I is encoded by a nuclear gene with enhanced transcript levels in flowers.";
Plant Mol. Biol. 31:1195-1204(1996).

Comments

FUNCTION: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).
CATALYTIC ACTIVITY: NADH + ubiquinone = NAD⁺ + ubiquinol.
CATALYTIC ACTIVITY: NADH + acceptor = NAD⁺ + reduced acceptor.
COFACTOR: Binds 1 4Fe-4S cluster (Potential).
SUBUNIT: Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme (By similarity).
SUBCELLULAR LOCATION: Mitochondrion.
SIMILARITY: Belongs to the complex I 20 kDa subunit family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X96671; CAA65451.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

T07603; T07603.

3D structure databases

ModBase

Q43844.

Family and domain databases

InterPro

IPR006138; NADH_DHase_20kDa_su.
IPR014406; NiFe_hyd_3_ssu/Q_oxred_NuoB.
IPR006137; OxRdtase_q6.
Graphical view of domain structure.

PANTHER

PTHR11995:SF2; NADH_DH_20kDa; 1.
PTHR11995; NiFe_hyd_3_ssu/Q_oxred_NuoB; 1.

Pfam

PF01058; Oxidored_q6; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR01957; nuoB_fam; 1.

PROSITE

PS01150; COMPLEX1_20K; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

4Fe-4S; Electron transport; Iron; Iron-sulfur; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Respiratory chain; Transit peptide; Transport; Ubiquinone.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 31`	`31`	`Mitochondrion (Potential).`
`CHAIN`	`32 213`	`182`	`NADH-ubiquinone oxidoreductase 20 kDa subunit, mitochondrial.`	`PRO_0000020035`
`METAL`	`88 88`		`Iron-sulfur (4Fe-4S) (Potential).`
`METAL`	`89 89`		`Iron-sulfur (4Fe-4S) (Potential).`
`METAL`	`153 153`		`Iron-sulfur (4Fe-4S) (Potential).`
`METAL`	`183 183`		`Iron-sulfur (4Fe-4S) (Potential).`

Sequence information

Length: 213 AA [This is the length of the unprocessed precursor]

Molecular weight: 23396 Da [This is the MW of the unprocessed precursor]

CRC64: 8047FAB323418FCB [This is a checksum on the sequence]

        10         20         30         40         50         60 
MALIARNAKL LTGTAPFLQR AATIHTTLPS LSQQPASSPA TSGGAQPPSM NTPAGISKPA 

        70         80         90        100        110        120 
EYVISKVDDL MNWARRGSIW PMTFGLACCA VEMMHAGAAR YDFDRFGIIF RPSPRQSDVM 

       130        140        150        160        170        180 
IVAGTLTNKM APALRKVYDQ MPEPRWVISM GSCANGGGYY HYSYAVVRGC DRIVPVDIYV 

       190        200        210 
PGCPPTAEAL LYGILQLQKK INRRKDLLMW WTQ

Q43844 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools