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[1]
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NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=7487048 [NCBI, ExPASy, EBI, Israel, Japan]
Dairi T.,
Asano Y.;
"Cloning, nucleotide sequencing, and expression of an opine dehydrogenase gene from Arthrobacter sp. strain 1C.";
Appl. Environ. Microbiol. 61:3169-3171(1995).
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[2]
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CHARACTERIZATION.
PubMed=2753861 [NCBI, ExPASy, EBI, Israel, Japan]
Asano Y.,
Yamaguchi K.,
Kondo K.;
"A new NAD+-dependent opine dehydrogenase from Arthrobacter sp. strain 1C.";
J. Bacteriol. 171:4466-4471(1989).
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[3]
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X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
DOI=10.1038/854; PubMed=9665174 [NCBI, ExPASy, EBI, Israel, Japan]
Britton K.L.,
Asano Y.,
Rice D.W.;
"Crystal structure and active site location of N-(1-D-carboxylethyl)-L-norvaline dehydrogenase.";
Nat. Struct. Biol. 5:593-601(1998).
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- FUNCTION: In the forward direction acts also on secondary amine dicarboxylates such as N-(1-carboxyethyl)methionine and N-(1-carboxyethyl)phenylalanine. In the reverse direction, the enzyme also acts on neutral amino acids as an amino donor. They include L-amino acids such as 2-aminopentanoic acid, 2-aminobutyric acid, 2-aminohexanoic acid, 3-chloroalanine, O-acetylserine, methionine, isoleucine, valine, phenylalanine, leucine and alanine.
- CATALYTIC ACTIVITY: (2S)-2-((1-(R)-carboxyethyl)amino)pentanoate + NAD+ + H2O = L-2-aminopentanoic acid + pyruvate + NADH.
- SUBUNIT: Homodimer.
- SIMILARITY: Belongs to the lysopine/nopaline/octopine/opine/vitopine dehydrogenases family.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 359 AA [This is the length of the unprocessed precursor] |
Molecular weight: 37937 Da [This is the MW of the unprocessed precursor] |
CRC64: 7542D8A44D859028 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MIESKTYAVL GLGNGGHAFA AYLALKGQSV LAWDIDAQRI KEIQDRGAII AEGPGLAGTA
70 80 90 100 110 120
HPDLLTSDIG LAVKDADVIL IVVPAIHHAS IAANIASYIS EGQLIILNPG ATGGALEFRK
130 140 150 160 170 180
ILRENGAPEV TIGETSSMLF TCRSERPGQV TVNAIKGAMD FACLPAAKAG WALEQIGSVL
190 200 210 220 230 240
PQYVAVENVL HTSLTNVNAV MHPLPTLLNA ARCESGTPFQ YYLEGITPSV GSLAEKVDAE
250 260 270 280 290 300
RIAIAKAFDL NVPSVCEWYK ESYGQSPATI YEAVQGNPAY RGIAGPINLN TRYFFEDVST
310 320 330 340 350
GLVPLSELGR AVNVPTPLID AVLDLISSLI DTDFRKEGRT LEKLGLSGLT AAGIRSAVE
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Q44297 in FASTA format |
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