NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1074/jbc.270.31.18252; PubMed=7543100 [NCBI, ExPASy, EBI, Israel, Japan]
Chlumsky L.J., Zhang L., Jorns M.S.;
"Sequence analysis of sarcosine oxidase and nearby genes reveals homologies with key enzymes of folate one-carbon metabolism.";
J. Biol. Chem. 270:18252-18259(1995).

Comments

FUNCTION: Catalyzes the oxidative demethylation of sarcosine to yield glycine, hydrogen peroxide and 5,10-methylenetetrahydrofolate.
CATALYTIC ACTIVITY: Sarcosine + H₂O + O₂ = glycine + formaldehyde + H₂O₂.
COFACTOR: FAD.
SUBUNIT: Heterotetramer (alpha 100 kDa, beta 42 kDa, gamma 20 kDa, and delta 6 kDa).
SUBCELLULAR LOCATION: Cytoplasm.
SIMILARITY: Belongs to the gcvT family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U23955; AAC43461.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

SMR

Q46337; 8-967.

ModBase

Q46337.

Enzyme and pathway databases

BioCyc

MetaCyc:MON-8521; -.

Family and domain databases

InterPro

IPR006076; FAD-dep_OxRdtase.
IPR013027; FAD_pyr_nucl-diS_OxRdtase.
IPR013977; GCV_T_C.
IPR006222; GCV_T_N.
IPR016040; NAD(P)-bd.
IPR001100; Pyr_nuc-diS_OxRdtase.
IPR006277; SoxA.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

Pfam

PF01266; DAO; 1.
PF01571; GCV_T; 1.
PF08669; GCV_T_C; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF037980; SoxA; 1.

PRINTS

PR00368; FADPNR.
PR00411; PNDRDTASEI.

TIGR01372; soxA; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cytoplasm; FAD; Flavoprotein; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 967`	`967`		`Sarcosine oxidase subunit alpha.`	`PRO_0000122627`

Sequence information

Length: 967 AA [This is the length of the unprocessed precursor]

Molecular weight: 102767 Da [This is the MW of the unprocessed precursor]

CRC64: C5F8A61F021134E9 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSQNTSYRLP AEQSPAARID RGEALVLTVD GKQLEAFRGD TVASAMLANG QRACGNSMYL 

        70         80         90        100        110        120 
DRPRGIFSAG VEEPNALVTV EARHEQDINE SMLAATTVPV TANLSATLLR GLGVLDPSTD 

       130        140        150        160        170        180 
PAYYDHVHVH TDVLVVGAGP AGLAAAREAS RSGARVLLLD ERAEAGGSLR DAAGEQIDGQ 

       190        200        210        220        230        240 
DAAAWIDATV AELAAAEETT HLQRTTVLGS YDANYVVAVQ RRTVHLDGPS GAGVSRERIW 

       250        260        270        280        290        300 
HIRANQVVLA TGAHERPIVF ENNDRPGIML AGAVRSYLNC YGVRAGSQIV VATTNDSAYP 

       310        320        330        340        350        360 
LVADLAASGG VVAVIDARTT VSAAAAEAVG AGVRVITGSV VVDTEANESG ELSAVIVAEL 

       370        380        390        400        410        420 
GEDRELGEPQ RFEADVLAVA GGFNPVVHLH SQRQGKLVWD TSIHAFVPDA AVANQHLAGA 

       430        440        450        460        470        480 
LTGLFDTASA LSTGAAVGAA AATAAGFERI AQVPQALPVP AGETRPVWLV PSLNGDQATN 

       490        500        510        520        530        540 
YTTHFVDLQR DQTVSDVLRA TGAGLESVEH IKRYTSISTA NDQGKTSGVA AIGVIAAVLG 

       550        560        570        580        590        600 
IENPAEIGTT TFRAPYTPVA FAALAGRTRG ELLDPARITP MHSWHLAQGA KFEDVGQWKR 

       610        620        630        640        650        660 
AWYFPQDGED MDAAVYRECA AVRESVGMLD ATTLGKIEIR GADAAEFLNR IYTNGYTKLK 

       670        680        690        700        710        720 
VGMARYGVMC KADGMVFDDG VTLRLAEDRF LMHTTTGGAA GVLDWLEEWL QTEWPELDVT 

       730        740        750        760        770        780 
CTSVTEQLAT VAVVGPRSRD VVAKLVTGLD VSNDAFKFMS FQDVTLDSGI EARISRISFS 

       790        800        810        820        830        840 
GELAYEIAIP SWHGLRVWED VYAAGQEFNI TPYGTETMHV LRAEKGFIIV GQDTDGTVTP 

       850        860        870        880        890        900 
QDAGMEWVVS KLKDFVGKRS FSREDNLRED RKHLVSVLPV DTALRLAEGA ALVADGAVET 

       910        920        930        940        950        960 
EGCTPMEGWV TSSYNSPALG RTFGLALIKN GRSRIGEVLK TPVNGQLVDV LVSDLVLFDP 


EGSRRDG

Q46337 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools