NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1128/JB.00810-06; PubMed=16980466 [NCBI, ExPASy, EBI, Israel, Japan]
Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.;
"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes.";
J. Bacteriol. 188:7922-7931(2006).

Comments

FUNCTION: Catalyzes the reversible reduction of methylene-H(4)MPT to methyl-H(4)MPT (By similarity).
CATALYTIC ACTIVITY: 5-methyltetrahydromethanopterin + coenzyme F420 = 5,10-methylenetetrahydromethanopterin + reduced coenzyme F420.
PATHWAY: One-carbon metabolism; methanogenesis from carbone dioxide; methyl-CoM from 5,10-methylene-H(4)MPT: step 1/2 .
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
SIMILARITY: Belongs to the mer family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000099; AAZ69238.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_303818.1; -.

3D structure databases

PDB

1Z69; X-ray; 2.61 A; A/B/C/D=1-327.

[ExPASy / RCSB / EBI]

1Z69; -.

Q46FV4; 1-327.

Enzyme and pathway databases

BioCyc

MBAR269797:MBAR_A0254-MON; -.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0018537;	Molecular function: coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01091; -; 1.
PBIL [Tree]

InterPro

IPR011251; Luciferase-like_bac.
IPR016048; Luciferase_mOase.
Graphical view of domain structure.

Gene3D

G3DSA:3.20.20.30; Luciferase_like; 1.

Pfam

PF00296; Bac_luciferase; 1.
Pfam graphical view of domain structure.

BLOCKS

Q46FV4.

Genome annotation databases

GeneID

3624867; -.

GenomeReviews

CP000099_GR; Mbar_A0254.

KEGG

mba:Mbar_A0254; -.

NMPDR

fig|269797.3.peg.370; -.

Phylogenomic databases

HOGENOM

Q46FV4; -.

Genome annotation databases

CMR

Q46FV4; Mbar_A0254.

Other

ProtoNet

Q46FV4.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Complete proteome; Cytoplasm; Methanogenesis; One-carbon metabolism; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 328`	`328`		`5,10-methylenetetrahydromethanopterin reductase.`	`PRO_1000064889`

Sequence information

Length: 328 AA [This is the length of the unprocessed precursor]

Molecular weight: 34592 Da [This is the MW of the unprocessed precursor]

CRC64: AC4BD2A64B93ADC7 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKFGIEFVPS DPALKIAYYA KLSEQQGFDH VWITDHYNNR DVYSTLTVLA LNTNSIKIGP 

        70         80         90        100        110        120 
GVTNSYTRNP AITASSIASI AEISGGRAVL GLGPGDKATF DAMGIAWKKP LATTKEAIQA 

       130        140        150        160        170        180 
IRDFISGKKV SMDGEMIKFA GAKLAFKAGN IPIYMGAQGP KMLELAGEIA DGVLINASHP 

       190        200        210        220        230        240 
KDFEVAVEQI KKGAEKAGRD PSEVDVTAYA CFSIDKDPVK AVNAAKVVVA FIVAGSPDLV 

       250        260        270        280        290        300 
LERHGIPVEA KSQIGAAIAK GDFGALMGGL VTPQMIEAFS ICGTPDDCMK RIKDLEAIGV 

       310        320 
TQIVAGSPIG PAKEKAIKLI GKEIIAKM

Q46FV4 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools