[1]	NUCLEOTIDE SEQUENCE [MRNA]. TISSUE=Testis; DOI=10.1016/j.mce.2004.11.009; PubMed=15836952 [NCBI, ExPASy, EBI, Israel, Japan] Rozman D., Seliskar M., Cotman M., Fink M.; "Pre-cholesterol precursors in gametogenesis."; Mol. Cell. Endocrinol. 234:47-56(2005).
[2]	NUCLEOTIDE SEQUENCE [MRNA]. DOI=10.1248/bpb.29.430; PubMed=16508140 [NCBI, ExPASy, EBI, Israel, Japan] Wang F., Shen Y., Song X., Xia G., Chen X., Zhou B., Lei L.; "cDNA cloning, genomic structure and expression analysis of the bovine lanosterol 14alpha-demethylase (CYP51) in gonads."; Biol. Pharm. Bull. 29:430-436(2006).
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. STRAIN=Hereford; TISSUE=Fetal medulla; NIH - Mammalian Gene Collection (MGC) project; Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalyzes C14-demethylation of lanosterol; it transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol.
CATALYTIC ACTIVITY: Obtusifoliol + 3 O₂ + 3 NADPH = 4-alpha-methyl-5-alpha-ergosta-8,14,24²⁸-trien-3-beta-ol + formate + 3 NADP⁺ + 4 H₂O.
COFACTOR: Heme group (By similarity).
PATHWAY: Steroid metabolism; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6 .
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane (By similarity). Microsome membrane (By similarity).
SIMILARITY: Belongs to the cytochrome P450 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

DQ078267; AAY82452.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
DQ085625; AAZ74630.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC149346; AAI49347.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001020490.1; -.

UniGene

Bt.49631

3D structure databases

ModBase

Q4PJW3.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0008398;	Molecular function: sterol 14-demethylase activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR001128; Cyt_P450.
IPR002403; Cyt_P450_E_grp-IV.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.630.10; Cyt_P450; 1.

PANTHER

PTHR19383; Cyt_P450; 1.

Pfam

PF00067; p450; 1.
Pfam graphical view of domain structure.

PRINTS

PR00465; EP450IV.
PR00385; P450.

PROSITE

PS00086; CYTOCHROME_P450; 1.

BLOCKS

Q4PJW3.

Genome annotation databases

Ensembl

ENSBTAG00000001992; Bos taurus. [Contig view]

GeneID

505060; -.

KEGG

bta:505060; -.

Phylogenomic databases

HOVERGEN

Q4PJW3; -.

Other

ProtoNet

Q4PJW3.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cholesterol biosynthesis; Endoplasmic reticulum; Heme; Iron; Lipid synthesis; Membrane; Metal-binding; Microsome; Monooxygenase; NADP; Oxidoreductase; Steroid biosynthesis; Sterol biosynthesis; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 502`	`502`	`Cytochrome P450 51A1.`	`PRO_0000051997`
`TRANSMEM`	`22 42`	`21`	`Potential.`
`METAL`	`448 448`		`Iron (heme axial ligand) (By similarity).`

Sequence information

Length: 502 AA [This is the length of the unprocessed precursor]

Molecular weight: 56596 Da [This is the MW of the unprocessed precursor]

CRC64: 5D1C5AFA43E067A1 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLDLLQAGGS VLGQAMEQVT GGNLASMLLI ACAFTLSLVY LFRLAVGHLA PPLPTGAKSP 

        70         80         90        100        110        120 
PYIVSPIPFL GHAIAFGKSP IEFLEDAYEK YGPVFSFTMV GKTFTYLLGS EAAALLFNSK 

       130        140        150        160        170        180 
NEDLNAEEVY SRLTTPVFGK GVAYDVPNTV FLEQKKMLKS GLNIAHFRQH VSIIEKETKE 

       190        200        210        220        230        240 
YFKSWGESGE KNLFEALSEL IILTASHCLH GKEIRSQLNE KVAQLYADLD GGFSHAAWLL 

       250        260        270        280        290        300 
PGWLPLPSFR RRDRAHREIK NIFYKAIQKR RESGEKIDDI LQTLLESTYK DGRPLTDDEV 

       310        320        330        340        350        360 
AGMLIGLLLA GQHTSSTTSA WMGFFLARDK TLQEKCFLEQ KTVCGENLPP LTYDQLKDLN 

       370        380        390        400        410        420 
LLDRCIKETL RLRPPIMTMM RLAKTPLTVA GYTIPPGHQV CVSPTVNQRL KDSWVERLDF 

       430        440        450        460        470        480 
NPDRYLEDSP ASGEKFAYVP FGAGRHRCIG ENFAYVQIKT IWSTMLRLYE FDLIDGYFPT 

       490        500 
VNYTTMIHTP EKPIIRYKRR SK

Q4PJW3 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools