[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. TISSUE=Temporal cortex; International consortium for macaque cDNA sequencing and analysis; "DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."; Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.

Comments

CATALYTIC ACTIVITY: 4,4-dimethyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O₂ = 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)⁺ + H₂O.
CATALYTIC ACTIVITY: 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O₂ = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)⁺ + 2 H₂O.
CATALYTIC ACTIVITY: 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)H + O₂ = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carboxylate + NAD(P)⁺ + H₂O.
COFACTOR: Iron (By similarity).
PATHWAY: Steroid metabolism; zymosterol biosynthesis; zymosterol from lanosterol: step 3/6 .
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein (Probable).
DOMAIN: The histidine box domains may contain the active site and/or be involved in metal ion binding.
SIMILARITY: Belongs to the sterol desaturase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AB169840; BAE01921.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q4R4Q4.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0000254;	Molecular function: C-4 methylsterol oxidase activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR006088; Sterol_desatur.
Graphical view of domain structure.

Pfam

PF01598; Sterol_desat; 1.
Pfam graphical view of domain structure.

BLOCKS

Q4R4Q4.

Phylogenomic databases

HOVERGEN

Q4R4Q4; -.

Other

ProtoNet

Q4R4Q4.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Endoplasmic reticulum; Iron; Lipid synthesis; Membrane; NAD; Oxidoreductase; Steroid biosynthesis; Sterol biosynthesis; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 293`	`293`	`C-4 methylsterol oxidase.`	`PRO_0000249850`
`TRANSMEM`	`55 75`	`21`	`Potential.`
`TRANSMEM`	`100 120`	`21`	`Potential.`
`TRANSMEM`	`199 219`	`21`	`Potential.`
`MOTIF`	`157 161`	`5`	`Histidine box-1.`
`MOTIF`	`170 174`	`5`	`Histidine box-2.`
`MOTIF`	`249 255`	`7`	`Histidine box-3.`

Sequence information

Length: 293 AA [This is the length of the unprocessed precursor]

Molecular weight: 35252 Da [This is the MW of the unprocessed precursor]

CRC64: 584AF62E7F3CC698 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MATNESVSIF SSASLAVEYV DSLLPENPLQ EPFKNAWNYM LNNYTKFQIA TWGSLIVHEA 

        70         80         90        100        110        120 
LYFSFCLPGF LFQFIPYMKK YKIQKDKPET WENQWKCFKV LLFNHFCIQL PLICGTYYFT 

       130        140        150        160        170        180 
EYFNIPYDWE RMPRWYFLLA RCFGCAVIED TWHYFMHRLL HHKRIYKYIH KVHHEFQAPF 

       190        200        210        220        230        240 
GMEAEYAHPL ETLILGTGFF IGIVLLCDHV ILLWAWVTIR LLETIDVHSG YDIPLNPLNL 

       250        260        270        280        290 
IPFYAGSRHH DFHHMNFIGN YASTFTWWDR IFGTDSQYHA YYEKKKKFEK KTE

Q4R4Q4 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools