[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=2CBS; PubMed=7530709 [NCBI, ExPASy, EBI, Israel, Japan] Haak B., Fetzner S., Lingens F.; "Cloning, nucleotide sequence, and expression of the plasmid-encoded genes for the two-component 2-halobenzoate 1,2-dioxygenase from Pseudomonas cepacia 2CBS."; J. Bacteriol. 177:667-675(1995).
[2]	PROTEIN SEQUENCE OF 2-21, AND CHARACTERIZATION. STRAIN=2CBS; PubMed=1370284 [NCBI, ExPASy, EBI, Israel, Japan] Fetzner S., Mueller R., Lingens F.; "Purification and some properties of 2-halobenzoate 1,2-dioxygenase, a two-component enzyme system from Pseudomonas cepacia 2CBS."; J. Bacteriol. 174:279-290(1992).

Comments

FUNCTION: Component of 2-halobenzoate dioxygenase multicomponent enzyme system which catalyzes the incorporation of both atoms of molecular oxygen into 2-halobenzoate to form catechol.
CATALYTIC ACTIVITY: 2-chlorobenzoate + NADH + O₂ = catechol + chloride + NAD⁺ + CO₂.
COFACTOR: Binds 1 2Fe-2S cluster per subunit (By similarity).
COFACTOR: Binds 1 Fe(2+) ion per subunit (By similarity).
PATHWAY: Xenobiotic degradation; benzoate degradation via CoA ligation .
SUBUNIT: Heterohexamer of 3 large (CbdA) subunits and 3 small (CbdB) subunits. The heterohexamer is part of 2-halobenzoate dioxygenase two component enzyme system. The other component is a NADH:acceptor reductase (CdbC).
SIMILARITY: Belongs to the bacterial ring-hydroxylating dioxygenase alpha subunit family.
SIMILARITY: Contains 1 Rieske domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X79076; CAA55681.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q51601.

Family and domain databases

InterPro

IPR005806; Rieske_reg.
IPR015881; Ring-hydroxy_dOase_2Fe2S_BS.
IPR015879; Ring_hydroxy_dOase_asu_C.
IPR001663; Rng_hydr_dOase-A.
Graphical view of domain structure.

Gene3D

G3DSA:2.102.10.10; Rieske_reg; 1.

PANTHER

PTHR21266:SF2; Rng_hydr_dOase-A; 1.

Pfam

PF00355; Rieske; 1.
PF00848; Ring_hydroxyl_A; 1.
Pfam graphical view of domain structure.

PROSITE

PS51296; RIESKE; 1.
PS00570; RING_HYDROXYL_ALPHA; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

2Fe-2S; Aromatic hydrocarbons catabolism; Dioxygenase; Direct protein sequencing; Iron; Iron-sulfur; Metal-binding; NAD; Oxidoreductase; Plasmid.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed.`
`CHAIN`	`2 465`	`464`	`2-halobenzoate 1,2-dioxygenase large subunit.`	`PRO_0000085051`
`DOMAIN`	`56 154`	`99`	`Rieske.`
`METAL`	`98 98`		`Iron-sulfur (2Fe-2S) (By similarity).`
`METAL`	`100 100`		`Iron-sulfur (2Fe-2S); via pros nitrogen (By similarity).`
`METAL`	`118 118`		`Iron-sulfur (2Fe-2S) (By similarity).`
`METAL`	`121 121`		`Iron-sulfur (2Fe-2S); via pros nitrogen (By similarity).`
`METAL`	`227 227`		`Iron (By similarity).`
`METAL`	`232 232`		`Iron (By similarity).`

Sequence information

Length: 465 AA [This is the length of the unprocessed precursor]

Molecular weight: 52478 Da [This is the MW of the unprocessed precursor]

CRC64: 08747DF6FB8D30DC [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSTPLIAGTG PSAVRQLISN AVQNDPVSGN FRCRRDIFTD AALFDYEMKY IFEQNWVFLA 

        70         80         90        100        110        120 
HESQVANPDD YLVSNIGRQP VIITRNKAGD VSAVINACSH RGAELCRRKQ GNRSTFTCQF 

       130        140        150        160        170        180 
HGWTFSNTGK LLKVKDGQDD NYPEGFNVDG SHDLTRIPSF ANYRGFLFGS MNPDACPIEE 

       190        200        210        220        230        240 
HLGGSKAILD QVIDQTPGEL EVLRGSSSYI YDGNWKLQIE NGADGYHVGS VHWNYVATIG 

       250        260        270        280        290        300 
RRDRTSDTIR TVDVTTWSKK NIGGTYTFEH GHMLLWTRLP NPEVRPVFAR REELKARVGE 

       310        320        330        340        350        360 
EVADAIVNQT RNLCIYPNLY VMDQISTQIR VVRPISVDKT EVTIYCFAPR DESEEVRNAR 

       370        380        390        400        410        420 
IRQYEDFFNV SGMGTPDDLE EFRACQSGYR GSAREWNDLS RGAPHWISGP DDNARRLGLA 

       430        440        450        460 
PLMSGARMED EGLFVQQHTY WAETMLRGIE AEPKVFNVQP VEVAQ

Q51601 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools