[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=2CBS; PubMed=7530709 [NCBI, ExPASy, EBI, Israel, Japan] Haak B., Fetzner S., Lingens F.; "Cloning, nucleotide sequence, and expression of the plasmid-encoded genes for the two-component 2-halobenzoate 1,2-dioxygenase from Pseudomonas cepacia 2CBS."; J. Bacteriol. 177:667-675(1995).
[2]	PROTEIN SEQUENCE OF 2-15, AND CHARACTERIZATION. STRAIN=2CBS; PubMed=1370284 [NCBI, ExPASy, EBI, Israel, Japan] Fetzner S., Mueller R., Lingens F.; "Purification and some properties of 2-halobenzoate 1,2-dioxygenase, a two-component enzyme system from Pseudomonas cepacia 2CBS."; J. Bacteriol. 174:279-290(1992).

Comments

FUNCTION: Component of 2-halobenzoate dioxygenase multicomponent enzyme system which catalyzes the incorporation of both atoms of molecular oxygen into 2-halobenzoate to form catechol.
CATALYTIC ACTIVITY: 2-chlorobenzoate + NADH + O₂ = catechol + chloride + NAD⁺ + CO₂.
PATHWAY: Xenobiotic degradation; benzoate degradation via CoA ligation .
SUBUNIT: Heterohexamer of 3 large (CbdA) subunits and 3 small (CbdB) subunits. The heterohexamer is part of 2-halobenzoate dioxygenase two component enzyme system. The other component is a NADH:acceptor reductase (CdbC).
SIMILARITY: Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X79076; CAA55682.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q51602.

Ontologies

GO:0018626;	Molecular function: 2-chlorobenzoate 1,2-dioxygenase activity (inferred from electronic annotation from EC).
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Family and domain databases

InterPro

IPR017641; Benzo_1-2-diOase_ssu.
IPR000391; Rng_hydr_dOase-B.
Graphical view of domain structure.

Pfam

PF00866; Ring_hydroxyl_B; 1.
Pfam graphical view of domain structure.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Aromatic hydrocarbons catabolism; Dioxygenase; Direct protein sequencing; NAD; Oxidoreductase; Plasmid.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed.`
`CHAIN`	`2 165`	`164`	`2-halobenzoate 1,2-dioxygenase small subunit.`	`PRO_0000085071`
`CONFLICT`	`12 12`		`V -> C (in Ref. 2; CAA55682).`

Sequence information

Length: 165 AA [This is the length of the unprocessed precursor]

Molecular weight: 19632 Da [This is the MW of the unprocessed precursor]

CRC64: 4886AF20AE127AA8 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTSLESSYLD VVAFIFREAR LLDDRSWDEW LECYDPEAVF WMPCWDDADT LVDDPRKHVS 

        70         80         90        100        110        120 
LIYYSDRMGL EDRVFRLRSE RSGASTPEPR TTHNIANVEI LERTERQIEA RFNWHTMNYR 

       130        140        150        160 
YKLLDHYFGT SFYTLKVSSS GLSILNKKVV LKNDLIHQVI DVYHV

Q51602 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools