|
|
|
|
|
|
[1]
|
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1074/jbc.272.7.4201; PubMed=9020134 [NCBI, ExPASy, EBI, Israel, Japan]
Yano T.,
Chu S.S.,
Sled' V.D.,
Ohnishi T.,
Yagi T.;
"The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit.";
J. Biol. Chem. 272:4201-4211(1997).
|
[2]
|
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Masui R.,
Kurokawa K.,
Nakagawa N.,
Tokunaga F.,
Koyama Y.,
Shibata T.,
Oshima T.,
Yokoyama S.,
Yasunaga T.,
Kuramitsu S.;
"Complete genome sequence of Thermus thermophilus HB8.";
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
|
|
|
|
- FUNCTION: NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.
- CATALYTIC ACTIVITY: NADH + quinone = NAD+ + quinol.
- SUBUNIT: NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.
- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
- SIMILARITY: Belongs to the complex I subunit 5 family.
|
|
|
|
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| Length: 606 AA [This is the length of the unprocessed precursor] |
Molecular weight: 65141 Da [This is the MW of the unprocessed precursor] |
CRC64: 47318E8F2665295C [This is a checksum on the sequence] |
|
10 20 30 40 50 60
MALLGTILLP LLGFALLGLF GKRMREPLPG VLASGLVLAS FLLGAGLLLS GGARFQAEWL
70 80 90 100 110 120
PGIPFSLLLD NLSGFMLLIV TGVGFLIHVY AIGYMGGDPG YSRFFAYFNL FIAMMLTLVL
130 140 150 160 170 180
ADSYPVMFIG WEGVGLASFL LIGFWYKNPQ YADSARKAFI VNRIGDLGFM LGMAILWALY
190 200 210 220 230 240
GTLSISELKE AMEGPLKNPD LLALAGLLLF LGAVGKSAQI PLMVWLPDAM AGPTPVSALI
250 260 270 280 290 300
HAATMVTAGV YLIARSSFLY SVLPDVSYAI AVVGLLTAAY GALSAFGQTD IKKIVAYSTI
310 320 330 340 350 360
SQLGYMFLAA GVGAYWVALF HVFTHAFFKA LLFLASGSVI HALGGEQDVR KMGGLWKHLP
370 380 390 400 410 420
QTRWHALIGA LALGGLPLLS GFWSKDAILA ATLTYPFGGV GFYVGALLVA VLTAMYAMRW
430 440 450 460 470 480
FVLVFLGEER GHHHPHEAPP VMLWPNHLLA LGSVLAGYLA LPHPLPNVLE PFLKPALAEV
490 500 510 520 530 540
EAHHLSLGAE WGLIALSAAV ALLGLWAGFV FFQRKVFPAW YLAFEAASRE AFYVDRAYNA
550 560 570 580 590 600
LIVNPLKALA EALFYGDRGL LSGYFGLGGA ARSLGQGLAR LQTGYLRVYA LLFVLGALLL
LGVMRW
|
Q56227 in FASTA format |
|
ExPASy Home page
CBR Canada
