NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=HTA426;
DOI=10.1093/nar/gkh970; PubMed=15576355 [NCBI, ExPASy, EBI, Israel, Japan]
Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I.;
"Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus.";
Nucleic Acids Res. 32:6292-6303(2004).

Comments

FUNCTION: Catalyzes the reduction of the double bond of an array of alpha, beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes (By similarity).
CATALYTIC ACTIVITY: NADPH + acceptor = NADP⁺ + reduced acceptor.
COFACTOR: FMN (By similarity).
SUBUNIT: Homotetramer (By similarity).
SIMILARITY: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. NamA subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BA000043; BAD76617.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_148185.1; -.

3D structure databases

ModBase

Q5KXG9.

Enzyme and pathway databases

BioCyc

GKAU235909:GK2332-MON; -.

Ontologies

GO:0003959;	Molecular function: NADPH dehydrogenase activity (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01614; -; 1.
PBIL [Tree]

InterPro

IPR013785; Aldolase_TIM.
IPR001155; OxRdtase_FMN_N.
Graphical view of domain structure.

Gene3D

G3DSA:3.20.20.70; Aldolase_TIM; 1.

Pfam

PF00724; Oxidored_FMN; 1.
Pfam graphical view of domain structure.

BLOCKS

Q5KXG9.

Genome annotation databases

3186211; -.

BA000043_GR; GK2332.

gka:GK2332; -.

fig|235909.3.peg.1192; -.

Phylogenomic databases

HOGENOM

Q5KXG9; -.

Genome annotation databases

CMR

Q5KXG9; GK2332.

Other

ProtoNet

Q5KXG9.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Flavoprotein; FMN; NADP; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 340`	`340`	`NADPH dehydrogenase.`	`PRO_0000216121`
`NP_BIND`	`23 27`	`5`	`FMN (By similarity).`
`BINDING`	`28 28`		`Substrate (By similarity).`
`BINDING`	`164 164`		`Substrate (By similarity).`
`BINDING`	`167 167`		`Substrate (By similarity).`
`BINDING`	`215 215`		`FMN (By similarity).`
`BINDING`	`308 308`		`FMN (By similarity).`

Sequence information

Length: 340 AA [This is the length of the unprocessed precursor]

Molecular weight: 37680 Da [This is the MW of the unprocessed precursor]

CRC64: A386C16F7EC573EE [This is a checksum on the sequence]

        10         20         30         40         50         60 
MNTMLFSPYT IRGLTLKNRI VMSPMCMYSC DTKDGAVRTW HKIHYPARAV GQVGLIIVEA 

        70         80         90        100        110        120 
TGVTPQGRIS ERDLGIWSDD HIAGLRELVG LVKEHGAAIG IQLAHAGRKS QVPGEIIAPS 

       130        140        150        160        170        180 
AVPFDDSSPT PKEMTKADIE ETVQAFQNGA RRAKEAGFDV IEIHAAHGYL INEFLSPLSN 

       190        200        210        220        230        240 
RRQDEYGGSP ENRYRFLGEV IDAVREVWDG PLFVRISASD YHPDGLTAKD YVPYAKRMKE 

       250        260        270        280        290        300 
QGVDLVDVSS GAIVPARMNV YPGYQVPFAE LIRREADIPT GAVGLITSGW QAEEILQNGR 

       310        320        330        340 
ADLVFLGREL LRNPYWPYAA ARELGAKISA PVQYERGWRF

Q5KXG9 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools