ID   NAMA_GEOKA              Reviewed;         340 AA.
AC   Q5KXG9;
DT   21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2005, sequence version 1.
DT   25-NOV-2008, entry version 21.
DE   RecName: Full=NADPH dehydrogenase;
DE            EC=1.6.99.1;
DE   AltName: Full=Xenobiotic reductase;
GN   Name=namA; OrderedLocusNames=GK2332;
OS   Geobacillus kaustophilus.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Geobacillus.
OX   NCBI_TaxID=1462;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HTA426;
RX   PubMed=15576355; DOI=10.1093/nar/gkh970;
RA   Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H.,
RA   Matsui S., Uchiyama I.;
RT   "Thermoadaptation trait revealed by the genome sequence of
RT   thermophilic Geobacillus kaustophilus.";
RL   Nucleic Acids Res. 32:6292-6303(2004).
CC   -!- FUNCTION: Catalyzes the reduction of the double bond of an array
CC       of alpha, beta-unsaturated aldehydes and ketones. It also reduces
CC       the nitro group of nitroester and nitroaromatic compounds. It
CC       could have a role in detoxification processes (By similarity).
CC   -!- CATALYTIC ACTIVITY: NADPH + acceptor = NADP(+) + reduced acceptor.
CC   -!- COFACTOR: FMN (By similarity).
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SIMILARITY: Belongs to the NADH:flavin oxidoreductase/NADH oxidase
CC       family. NamA subfamily.
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DR   EMBL; BA000043; BAD76617.1; -; Genomic_DNA.
DR   RefSeq; YP_148185.1; -.
DR   GeneID; 3186211; -.
DR   GenomeReviews; BA000043_GR; GK2332.
DR   KEGG; gka:GK2332; -.
DR   NMPDR; fig|235909.3.peg.1192; -.
DR   HOGENOM; Q5KXG9; -.
DR   BioCyc; GKAU235909:GK2332-MON; -.
DR   GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR   GO; GO:0003959; F:NADPH dehydrogenase activity; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01614; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR001155; OxRdtase_FMN_N.
DR   Gene3D; G3DSA:3.20.20.70; Aldolase_TIM; 1.
DR   Pfam; PF00724; Oxidored_FMN; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Flavoprotein; FMN; NADP; Oxidoreductase.
FT   CHAIN         1    340       NADPH dehydrogenase.
FT                                /FTId=PRO_0000216121.
FT   NP_BIND      23     27       FMN (By similarity).
FT   BINDING      28     28       Substrate (By similarity).
FT   BINDING     164    164       Substrate (By similarity).
FT   BINDING     167    167       Substrate (By similarity).
FT   BINDING     215    215       FMN (By similarity).
FT   BINDING     308    308       FMN (By similarity).
SQ   SEQUENCE   340 AA;  37680 MW;  A386C16F7EC573EE CRC64;
     MNTMLFSPYT IRGLTLKNRI VMSPMCMYSC DTKDGAVRTW HKIHYPARAV GQVGLIIVEA
     TGVTPQGRIS ERDLGIWSDD HIAGLRELVG LVKEHGAAIG IQLAHAGRKS QVPGEIIAPS
     AVPFDDSSPT PKEMTKADIE ETVQAFQNGA RRAKEAGFDV IEIHAAHGYL INEFLSPLSN
     RRQDEYGGSP ENRYRFLGEV IDAVREVWDG PLFVRISASD YHPDGLTAKD YVPYAKRMKE
     QGVDLVDVSS GAIVPARMNV YPGYQVPFAE LIRREADIPT GAVGLITSGW QAEEILQNGR
     ADLVFLGREL LRNPYWPYAA ARELGAKISA PVQYERGWRF
//