Methylenetetrahydrofolate dehydrogenase
     (EC 1.5.1.5)
Methenyltetrahydrofolate cyclohydrolase
     (EC 3.5.4.9)
Formyltetrahydrofolate synthetase
     (EC 6.3.4.3)

Gene name

Name:

MTHFD1

From

Pongo abelii (Sumatran orangutan)

[TaxID: 9601]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Pongo.

Protein existence

2: Evidence at transcript level;

References

[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. TISSUE=Kidney; The German cDNA consortium; Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.

Comments

CATALYTIC ACTIVITY: 5,10-methylenetetrahydrofolate + NADP⁺ = 5,10-methenyltetrahydrofolate + NADPH.
CATALYTIC ACTIVITY: 5,10-methenyltetrahydrofolate + H₂O = 10-formyltetrahydrofolate.
CATALYTIC ACTIVITY: ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.
PATHWAY: One-carbon metabolism; tetrahydrofolate pathway .
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
DOMAIN: This trifunctional enzyme consists of two major domains: a N-terminal part, containing the methylene-THF dehydrogenase and the methenyl-THF cyclohydrolase activities and a larger formyl-THF synthetase domain.
SIMILARITY: In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
SIMILARITY: In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CR859711; CAH91870.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

SMR

Q5R8P0; 2-296.

ModBase

Q5R8P0.

Family and domain databases

InterPro

IPR000559; Fmtethyd_synth.
IPR016040; NAD(P)-bd.
IPR000672; THF_DHase/CycOHase.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

Pfam

PF01268; FTHFS; 1.
PF00763; THF_DHG_CYH; 1.
PF02882; THF_DHG_CYH_C; 1.
Pfam graphical view of domain structure.

PRINTS

PR00085; THFDHDRGNASE.

ProDom

PD002300; THFDhg/Cyc_hydro; 1.
[Domain structure / List of seq. sharing at least 1 domain]

PROSITE

PS00721; FTHFS_1; 1.
PS00722; FTHFS_2; 1.
PS00766; THF_DHG_CYH_1; 1.
PS00767; THF_DHG_CYH_2; 1.

BLOCKS

Q5R8P0.

Phylogenomic databases

HOVERGEN

Q5R8P0; -.

Other

ProtoNet

Q5R8P0.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Amino-acid biosynthesis; ATP-binding; Cytoplasm; Histidine biosynthesis; Hydrolase; Ligase; Methionine biosynthesis; Multifunctional enzyme; NADP; Nucleotide-binding; One-carbon metabolism; Oxidoreductase; Phosphoprotein; Purine biosynthesis.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed (By similarity).`
`CHAIN`	`2 935`	`934`	`C-1-tetrahydrofolate synthase, cytoplasmic.`	`PRO_0000265955`
`NP_BIND`	`172 174`	`3`	`NADP (By similarity).`
`NP_BIND`	`380 387`	`8`	`ATP (By similarity).`
`REGION`	`2 305`	`304`	`Methylenetetrahydrofolate dehydrogenase and cyclohydrolase (By similarity).`
`REGION`	`52 56`	`5`	`Substrate binding (By similarity).`
`REGION`	`99 101`	`3`	`Substrate binding (By similarity).`
`REGION`	`272 276`	`5`	`Substrate binding (By similarity).`
`REGION`	`306 935`	`630`	`Formyltetrahydrofolate synthetase.`
`BINDING`	`197 197`		`NADP (By similarity).`
`MOD_RES`	`786 786`		`Phosphothreonine (By similarity).`

Sequence information

Length: 935 AA [This is the length of the unprocessed precursor]

Molecular weight: 101341 Da [This is the MW of the unprocessed precursor]

CRC64: 4D98F58C5A908632 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAPAEILNGR EISAQIRARL KNQVTQLKEQ VPGFTPGLAI LLVGNRDDSN LYINVKLKAA 

        70         80         90        100        110        120 
EEIGIKATHI KLPRTTTESE VIKYITSLNE DSTVHGFLVQ LPLDSENSIN TEEVINAIAP 

       130        140        150        160        170        180 
EKDVDGLTSI SAGKLARGDL NDCFIPCTPK GCLELIKETG VPIAGRHAVV VGRSKIVGAP 

       190        200        210        220        230        240 
MHDLLLWNNA TVTTCHSKTA NLDEEVNKGD ILVVATGRPE MVKGEWIKPG AIVIDCGINY 

       250        260        270        280        290        300 
VPDDKKPNGR KVVGDVAYDE AKERASFITP VPGGVGPMTV AMLMQSTVES AKRFLEKFKP 

       310        320        330        340        350        360 
GKWMIQYNNL NLKTPDPSDI DISRSCKPKP IGKLAREIGL LSEEVELYGE TKAKVLLSAL 

       370        380        390        400        410        420 
ERLKHRPDGK YVVVTGITPT PLGEGKSTTT VGLVQALGAH LYQNVFACVR QPSQGPTFGI 

       430        440        450        460        470        480 
KGGAAGGGYS QVIPMEEFNL HLTGDIHAFT AANNLVAAAI DARIFHELTQ TDKALFNRLV 

       490        500        510        520        530        540 
PSVNGVRKFS DIQIRRLKRL GIEKTDPTTL TDEEINRFAR LDIDPETITW QRVLDTNDRF 

       550        560        570        580        590        600 
LRKITIGQAP TEKGHTRTAQ FDISVASEIM AALALTTSLE DMRERLGKMV VASSKKGEPV 

       610        620        630        640        650        660 
SAEDLGVSGA LTVLMKDAIK PNLMQTLEGT PVFVHAGPFA NIAHGNSSII ADRIALKLVG 

       670        680        690        700        710        720 
PEGFVVTEAG FGADIGMEKF FNIKCRYSGL CPHVVVLVAT VRALKMHGGG PTVTAGLPLP 

       730        740        750        760        770        780 
KAYIEENLEL VEKGFSNLKK QIENARMFGI PVVVAVNAFK TDTEAELDLI SRLSREHGAF 

       790        800        810        820        830        840 
DAVKCTHWAE GGNGALALAQ AVQRAAQAPS SFQLLYDLKL PVEDKIRIIA QKIYGADDIE 

       850        860        870        880        890        900 
LLPEAQHKAE VYTKQGFGNL PVCMAKTHLS LSHNPEQKGV PTGFILPIRD IRASVGAGFL 

       910        920        930 
YPLVGTMSTM PGLPTRPCFY DIDLDPETQQ VNGLF

Q5R8P0 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools