NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC BAA-946 / MGAS10394 / Serotype M6;
DOI=10.1086/422697; PubMed=15272401 [NCBI, ExPASy, EBI, Israel, Japan]
Banks D.J., Porcella S.F., Barbian K.D., Beres S.B., Philips L.E., Voyich J.M., DeLeo F.R., Martin J.M., Somerville G.A., Musser J.M.;
"Progress toward characterization of the group A Streptococcus metagenome: complete genome sequence of a macrolide-resistant serotype M6 strain.";
J. Infect. Dis. 190:727-738(2004).

[2]

PROTEIN SEQUENCE OF 75-89; 107-121; 127-143; 163-181 AND 303-315, AND MASS SPECTROMETRY.
STRAIN=JRS4 / Serotype M6;
Hogan D.A., Du P., Stevenson T.I., Whitton M., Kilby G.W., Rogers J., VanBogelen R.A.;
"Two-dimensional gel electrophoresis map of Streptococcus pyogenes proteins.";
Submitted (MAY-2000) to UniProtKB.

Comments

FUNCTION: Catalyzes the four-electron reduction of molecular oxygen to water (By similarity).
CATALYTIC ACTIVITY: NADH + acceptor = NAD⁺ + reduced acceptor.
COFACTOR: Binds 1 FAD per subunit (By similarity).
MASS SPECTROMETRY: Mass=49603.57; Method=Electrospray; Range=1-456; Source=Ref.2;.
MISCELLANEOUS: The active site is the redox-active Cys-44 oxidized to Cys-SOH. The oxidized form is stabilized by an hydrogen bond formation with His-11 (By similarity).
SIMILARITY: Belongs to the class-III pyridine nucleotide-disulfide oxidoreductase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000003; AAT87003.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_060186.1; -.

3D structure databases

SMR

Q5XC60; 2-456.

ModBase

Q5XC60.

Enzyme and pathway databases

BioCyc

SPYO286636:M6_SPY0868-MON; -.

Family and domain databases

InterPro

IPR013027; FAD_pyr_nucl-diS_OxRdtase.
IPR001100; Pyr_nuc-diS_OxRdtase.
IPR004099; Pyr_nucl-diS_OxRdtase_dimer.
IPR001327; Pyr_OxRdtase_NAD_bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.30.390.30; Pyr_redox_dim; 1.

Pfam

PF00070; Pyr_redox; 1.
PF07992; Pyr_redox_2; 1.
PF02852; Pyr_redox_dim; 1.
Pfam graphical view of domain structure.

PRINTS

PR00368; FADPNR.
PR00411; PNDRDTASEI.

ProDom

PD000139; FAD_pyr_redox; 1.
[Domain structure / List of seq. sharing at least 1 domain]

BLOCKS

Q5XC60.

Genome annotation databases

GeneID

2941962; -.

GenomeReviews

CP000003_GR; M6_Spy0868.

KEGG

spa:M6_Spy0868; -.

Phylogenomic databases

HOGENOM

Q5XC60; -.

Genome annotation databases

CMR

Q5XC60; M6_Spy0868.

Other

ProtoNet

Q5XC60.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Direct protein sequencing; FAD; Flavoprotein; NAD; Oxidation; Oxidoreductase; Redox-active center.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 456`	`456`	`Probable NADH oxidase.`	`PRO_0000259672`
`NP_BIND`	`8 12`	`5`	`FAD (By similarity).`
`NP_BIND`	`110 113`	`4`	`FAD (By similarity).`
`NP_BIND`	`163 178`	`16`	`NAD (By similarity).`
`NP_BIND`	`282 292`	`11`	`FAD (By similarity).`
`ACT_SITE`	`11 11`		`Proton acceptor (By similarity).`
`ACT_SITE`	`44 44`		`Redox-active (By similarity).`
`BINDING`	`44 44`		`FAD (By similarity).`
`BINDING`	`171 171`		`NAD; via amide nitrogen (By similarity).`
`BINDING`	`190 190`		`NAD (By similarity).`
`BINDING`	`199 199`		`NAD (By similarity).`
`BINDING`	`254 254`		`NAD; via amide nitrogen (By similarity).`
`BINDING`	`292 292`		`FAD (By similarity).`
`BINDING`	`308 308`		`NAD; via carbonyl oxygen (By similarity).`
`BINDING`	`310 310`		`FAD; via amide nitrogen (By similarity).`
`BINDING`	`339 339`		`NAD; via carbonyl oxygen (By similarity).`
`MOD_RES`	`44 44`		`Cysteine sulfenic acid (-SOH) (By similarity).`
`CONFLICT`	`177 177`		`E -> V (in Ref. 2; AA sequence).`

Sequence information

Length: 456 AA [This is the length of the unprocessed precursor]

Molecular weight: 49636 Da [This is the MW of the unprocessed precursor]

CRC64: 782106FDC8945744 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSKIVVVGAN HAGTACIKTM LTNYGDANEI VVFDQNSNIS FLGCGMALWI GEQIAGPEGL 

        70         80         90        100        110        120 
FYSDKEELES LGAKVYMESP VQSIDYDAKT VTALVDGKNH VETYDKLIFA TGSQPILPPI 

       130        140        150        160        170        180 
KGAEIKEGSL EFEATLENLQ FVKLYQNSAD VIAKLENKDI KRVAVVGAGY IGVELAEAFQ 

       190        200        210        220        230        240 
RKGKEVVLID VVDTCLAGYY DRDLTDLMAK NMEEHGIQLA FGETVKEVAG NGKVEKIITD 

       250        260        270        280        290        300 
KNEYDVDMVI LAVGFRPNTT LGNGKIDLFR NGAFLVNKRQ ETSIPGVYAI GDCATIYDNA 

       310        320        330        340        350        360 
TRDTNYIALA SNAVRTGIVA AHNACGTDLE GIGVQGSNGI SIYGLHMVST GLTLEKAKRL 

       370        380        390        400        410        420 
GFDAAVTEYT DNQKPEFIEH GNFPVTIKIV YDKDSRRILG AQMAAREDMS MGIHMFSLAI 

       430        440        450 
QEGVTIEKLA LTDIFFLPHF NKPYNYITMA ALGAKD

Q5XC60 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools