ExPASy Home page

Site Map

Search ExPASy

Contact us

Swiss-Prot

The ExPASy Server requires Javascript to be fully functional. You may not see all the information available for this page (More information).

UniProtKB/Swiss-Prot entry Q60HE3

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

Note: most headings are clickable, even if they don't appear as links. They link to the user manual or other documents.

Entry information

Entry name

NDUS8_MACFA

Primary accession number

Q60HE3

Secondary accession numbers

None

Integrated into Swiss-Prot on

March 29, 2005

Sequence was last modified on

November 23, 2004 (Sequence version 1)

Annotations were last modified on

July 22, 2008 (Entry version 26)

Name and origin of the protein

Protein name

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial [Precursor]

Synonyms

EC 1.6.5.3
EC 1.6.99.3
NADH-ubiquinone oxidoreductase 23 kDa subunit
Complex I-23kD
CI-23kD

Gene name

	Name:	NDUFS8
	ORFNames:	QorA-12386

From

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

[TaxID: 9541]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Cercopithecidae; Cercopithecinae; Macaca.

Protein existence

2: Evidence at transcript level;

References

[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. TISSUE=Occipital cortex; Kusuda J., Osada N., Tanuma R., Hirata M., Sugano S., Hashimoto K.; "Isolation and characterization of cDNA for macaque neurological disease genes."; Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).
CATALYTIC ACTIVITY: NADH + ubiquinone = NAD⁺ + ubiquinol.
CATALYTIC ACTIVITY: NADH + acceptor = NAD⁺ + reduced acceptor.
COFACTOR: Binds 2 4Fe-4S clusters per subunit (By similarity).
SUBUNIT: Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme (By similarity).
SUBCELLULAR LOCATION: Mitochondrion (By similarity).
SIMILARITY: Belongs to the complex I 23 kDa subunit family.
SIMILARITY: Contains 2 4Fe-4S ferredoxin-type domains.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AB125184; BAD51972.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q60HE3.

Ontologies

GO:0005747;	Cellular component: mitochondrial respiratory chain complex I (inferred from sequence or structural similarity from UniProtKB).
GO:0032981;	Biological process: mitochondrial respiratory chain complex I assembly (inferred from sequence or structural similarity from UniProtKB).
GO:0006979;	Biological process: response to oxidative stress (inferred from sequence or structural similarity from UniProtKB).
QuickGo view.

Family and domain databases

InterPro

IPR001450; 4Fe4S_Fe_S_bd.
IPR010226; NADH_quinone_OxRdtase_I.
Graphical view of domain structure.

Pfam

PF00037; Fer4; 2.
Pfam graphical view of domain structure.

PRINTS

PR00353; 4FE4SFRDOXIN.

TIGRFAMs

TIGR01971; NuoI; 1.

PROSITE

PS00198; 4FE4S_FER_1; 2.
PS51379; 4FE4S_FER_2; 2.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q60HE3.

Phylogenomic databases

HOVERGEN

Q60HE3; -.

Other

ProtoNet

Q60HE3.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

4Fe-4S; Electron transport; Iron; Iron-sulfur; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Repeat; Respiratory chain; Transit peptide; Transport; Ubiquinone.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 34`	`34`	`Mitochondrion (By similarity).`
`CHAIN`	`35 210`	`176`	`NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial.`	`PRO_0000020013`
`DOMAIN`	`102 131`	`30`	`4Fe-4S ferredoxin-type 1.`
`DOMAIN`	`141 170`	`30`	`4Fe-4S ferredoxin-type 2.`
`METAL`	`111 111`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`114 114`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`117 117`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`121 121`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`150 150`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`153 153`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`156 156`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`160 160`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`

Sequence information

Length: 210 AA [This is the length of the unprocessed precursor]

Molecular weight: 23689 Da [This is the MW of the unprocessed precursor]

CRC64: 29F1E397F5B75045 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MRCLTMPTLL RALAQAAHTG PPGGRTLHSS AVAATYKYVN MQESKTDMKS VTDRAARTLL 

        70         80         90        100        110        120 
WTELFRGLGM TLSYLFREPA TINYPFEKGP LSPRFRGEHA LRRYPSGEER CIACKLCEAV 

       130        140        150        160        170        180 
CPAQAITIEA EPRADGSRRT TRYDIDMTKC IYCGFCQEAC PVDAIVEGPN FEFSTETHEE 

       190        200        210 
LLYNKEKLLN NGDKWEAEIA ANIQADYLYR

Q60HE3 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools