[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. STRAIN=Brown Norway; TISSUE=Testis; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).
[2]	PROTEIN SEQUENCE OF 57-73; 76-96; 97-107; 128-138; 159-166; 172-179; 210-216; 255-266; 272-323; 343-350; 384-404 AND 426-437. STRAIN=Wistar; TISSUE=Olfactory bulb; Maurya D.K., Bhargava P.; Submitted (MAY-2008) to UniProtKB.

Comments

FUNCTION: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).
CATALYTIC ACTIVITY: NADH + ubiquinone = NAD⁺ + ubiquinol.
CATALYTIC ACTIVITY: NADH + acceptor = NAD⁺ + reduced acceptor.
COFACTOR: Binds 1 4Fe-4S cluster.
SUBUNIT: Complex I is composed of 45 different subunits. Component of the iron-sulfur (IP) fragment of the enzyme (By similarity).
SUBCELLULAR LOCATION: Mitochondrion inner membrane; Peripheral membrane protein; Matrix side (By similarity).
SIMILARITY: Belongs to the complex I 49 kDa subunit family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BC082067; AAH82067.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001011907.1; -.

UniGene

Rn.99666

3D structure databases

ModBase

Q641Y2.

Organism-specific databases

RGD

1307109; Ndufs2.

Family and domain databases

InterPro

IPR014029; NADH-UbQ_OxRdtase_49kDa_CS.
IPR010219; NADH_DH_1_dsu.
IPR001135; NADH_UbQ_OxRdtase_49kDa.
Graphical view of domain structure.

Pfam

PF00346; Complex1_49kDa; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR01962; NuoD; 1.

PROSITE

PS00535; COMPLEX1_49K; 1.

BLOCKS

Q641Y2.

Genome annotation databases

GeneID

289218; -.

KEGG

rno:289218; -.

Phylogenomic databases

HOVERGEN

Q641Y2; -.

Other

ProtoNet

Q641Y2.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

4Fe-4S; Direct protein sequencing; Electron transport; Iron; Iron-sulfur; Membrane; Metal-binding; Mitochondrion; Mitochondrion inner membrane; NAD; Oxidoreductase; Respiratory chain; Transit peptide; Transport; Ubiquinone.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 33`	`33`	`Mitochondrion (By similarity).`
`CHAIN`	`34 463`	`430`	`NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial.`	`PRO_0000342702`
`METAL`	`326 326`		`Iron-sulfur (4Fe-4S) (Potential).`
`METAL`	`332 332`		`Iron-sulfur (4Fe-4S) (Potential).`
`METAL`	`347 347`		`Iron-sulfur (4Fe-4S) (Potential).`

Sequence information

Length: 463 AA [This is the length of the unprocessed precursor]

Molecular weight: 52562 Da [This is the MW of the unprocessed precursor]

CRC64: B9919E581C229C19 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAALRAVCSL RGVGAQVLRA GSGIRLPSQP SRGARRWQPD IEWAEQFSGA VMYPSKETAH 

        70         80         90        100        110        120 
WKPPPWNDVD VLKEKVVTNV TLNFGPQHPA AHGVLRLVLE LSGEMVRKCD PHIGLLHRGT 

       130        140        150        160        170        180 
EKLIEYKTYL QALPYFDRLD YVSMMCNEQA YSLAVEKLLN IQPPPRAQWI RVLFGEITRI 

       190        200        210        220        230        240 
LNHIMAVTTH ALDIGAMTPF FWMFEEREKM FEFYERVSGA RMHAAYIRPG GVHQDLPLGL 

       250        260        270        280        290        300 
MDDIYEFSKN FSLRIDEVEE MLTNNRIWRN RTVDIGVVSA EDALNYGFSG VMLRGSGIQW 

       310        320        330        340        350        360 
DLRKTQPYDV YDQVEFDVPI GSRGDCYDRY LCRVEEMRQS LRIIEQCLNK MPPGEIKVDD 

       370        380        390        400        410        420 
AKVSPPKRAE MKTSMESLIH HFKLYTEGYQ VPPGATYTAI EAPKGEFGVY LVSDGSSRPY 

       430        440        450        460 
RCKIKAPGFA HLAGLDKMSK GHMLADVVAI IGTQDIVFGE IDR

Q641Y2 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools