NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=Dunkin-Hartley;
TISSUE=Adrenal gland;
DOI=10.1006/bbrc.1996.0591; PubMed=8619851 [NCBI, ExPASy, EBI, Israel, Japan]
Buelow H.E., Mobius K., Bahr V., Bernhardt R.;
"Molecular cloning and functional expression of the cytochrome P450 11B-hydroxylase of the guinea pig.";
Biochem. Biophys. Res. Commun. 221:304-312(1996).

Comments

FUNCTION: Has steroid 11-beta-hydroxylase activity. In addition to this activity, the 18 or 19-hydroxylation of steroids and the aromatization of androstendione to estrone have also been ascribed to cytochrome P450 XIB.
CATALYTIC ACTIVITY: A steroid + reduced adrenal ferredoxin + O₂ = an 11-beta-hydroxysteroid + oxidized adrenal ferredoxin + H₂O.
COFACTOR: Heme group (By similarity).
SUBCELLULAR LOCATION: Mitochondrion membrane.
SIMILARITY: Belongs to the cytochrome P450 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

Z69785; CAA93633.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

JC4709; JC4709.

3D structure databases

HSSP

P00189; 1SCC. [HSSP ENTRY / PDB]

ModBase

Q64408.

Ontologies

GO:0031966;	Cellular component: mitochondrial membrane (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.

Family and domain databases

InterPro

IPR001128; Cyt_P450.
IPR002401; Cyt_P450_E_grp-I.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.630.10; Cyt_P450; 1.

PANTHER

PTHR19383; Cyt_P450; 1.

Pfam

PF00067; p450; 1.
Pfam graphical view of domain structure.

PRINTS

PR00463; EP450I.
PR00385; P450.

PROSITE

PS00086; CYTOCHROME_P450; 1.

BLOCKS

Q64408.

Genome annotation databases

Ensembl

ENSCPOG00000013558; Cavia porcellus. [Contig view]

Phylogenomic databases

HOVERGEN

Q64408; -.

Other

ProtoNet

Q64408.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Heme; Iron; Lipid metabolism; Membrane; Metal-binding; Mitochondrion; Monooxygenase; Oxidoreductase; Steroid metabolism; Steroidogenesis; Transit peptide.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 24`	`24`	`Mitochondrion.`
`CHAIN`	`25 500`	`476`	`Cytochrome P450 11B1, mitochondrial.`	`PRO_0000003595`
`METAL`	`447 447`		`Iron (heme axial ligand) (By similarity).`

Sequence information

Length: 500 AA [This is the length of the unprocessed precursor]

Molecular weight: 57820 Da [This is the MW of the unprocessed precursor]

CRC64: C4B5AB6041CD4AF2 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAFRLKSDVR LAGSWLCLRG ARALGTRAVT ASKASVLPFE VIPQHQGNKR QRVLQFWKEQ 

        70         80         90        100        110        120 
NHDDLHLEMH QTFQELGPIF RCDVGSTRIV AVMLPEDCAR LHQAESPYPH RMHLEPWMAY 

       130        140        150        160        170        180 
REHRRQNLGV FLLNGPEWLS NRRWLNPNVL SPKAVQNLLP MVDTVARDFS EALKQKVLQS 

       190        200        210        220        230        240 
AQGSLTMDMQ PDIHKYTVEV SNFALFGERL GLFGCNPSSQ SLKFIHALEA VFKTTTQLMF 

       250        260        270        280        290        300 
LPRSLSRWMR SQAWKEHFEA WDYISEYAEN RIQKKYEELA RGCSQYNSIV ANLMLQGNLP 

       310        320        330        340        350        360 
LRAMKANIMD LVAGSVDTTA LPLMMTLFEL ARNPTVQQAL RQESMATEPN IYENPQRLRM 

       370        380        390        400        410        420 
ELPLLWAAIK ETLRMYPVGL FLERFLTSPL VLQNYHIPAG TLVHLNLYSM GRNPEVFLSP 

       430        440        450        460        470        480 
EHYNPQRWLE NKETYKHLAF GFGVRQCIGR RLAEVEMLLF LHHVLKSFCV ETAFQEDVKF 

       490        500 
AYRFVMMPTS APLLTFRPVS

Q64408 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools