NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/nbt1010; PubMed=15378067 [NCBI, ExPASy, EBI, Israel, Japan]
Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J.;
"The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens.";
Nat. Biotechnol. 22:1275-1281(2004).

Comments

FUNCTION: Plays a role in nitrite reduction (By similarity).
CATALYTIC ACTIVITY: NH₃ + 2 H₂O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H⁺.
COFACTOR: Binds 1 calcium ion per monomer (By similarity).
COFACTOR: Binds 5 heme groups covalently per monomer (By similarity).
PATHWAY: Nitrogen metabolism; nitrate reduction (assimilation).
SUBCELLULAR LOCATION: Periplasm (By similarity).
SIMILARITY: Belongs to the cytochrome c-552 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AE016827; AAU38426.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_089011.1; -.

3D structure databases

ModBase

Q65RI4.

Ontologies

GO:0042597;	Cellular component: periplasmic space (inferred from electronic annotation from HAMAP).
GO:0042279;	Molecular function: nitrite reductase (cytochrome, ammonia-forming) activity (inferred from electronic annotation from HAMAP).
GO:0006807;	Biological process: nitrogen compound metabolic process (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01182; -; 1.
PBIL [Tree]

InterPro

IPR003321; Cyt_c552.
IPR017570; Cytc_552_NO2Rdtase_formate-dep.
IPR011031; Multihaem_cyt.
Graphical view of domain structure.

Pfam

PF02335; Cytochrom_C552; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000243; Cyt_c552; 1.

PROSITE

PS51008; MULTIHEME_CYTC; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q65RI4.

Genome annotation databases

3075137; -.

AE016827_GR; MS1819.

msu:MS1819; -.

fig|221988.1.peg.1708; -.

Phylogenomic databases

HOGENOM

Q65RI4; -.

Genome annotation databases

CMR

Q65RI4; MS1819.

Other

ProtoNet

Q65RI4.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Calcium; Complete proteome; Electron transport; Heme; Iron; Metal-binding; Oxidoreductase; Periplasm; Signal; Transport.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 32`	`32`	`Potential.`
`CHAIN`	`33 525`	`493`	`Cytochrome c-552.`	`PRO_0000268968`
`METAL`	`129 129`		`Iron (heme 3 axial ligand) (By similarity).`
`METAL`	`161 161`		`Iron (heme 1 axial ligand) (By similarity).`
`METAL`	`199 199`		`Iron (heme 2 axial ligand) (By similarity).`
`METAL`	`248 248`		`Iron (heme 3 axial ligand) (By similarity).`
`METAL`	`250 250`		`Calcium (By similarity).`
`METAL`	`251 251`		`Calcium; via carbonyl oxygen (By similarity).`
`METAL`	`298 298`		`Calcium; via carbonyl oxygen (By similarity).`
`METAL`	`300 300`		`Calcium (By similarity).`
`METAL`	`312 312`		`Iron (heme 5 axial ligand) (By similarity).`
`METAL`	`323 323`		`Iron (heme 4 axial ligand) (By similarity).`
`METAL`	`338 338`		`Iron (heme 2 axial ligand) (By similarity).`
`METAL`	`357 357`		`Iron (heme 5 axial ligand) (By similarity).`
`METAL`	`432 432`		`Iron (heme 4 axial ligand) (By similarity).`
`BINDING`	`157 157`		`Heme 1 (covalent) (By similarity).`
`BINDING`	`160 160`		`Heme 1 (covalent) (By similarity).`
`BINDING`	`195 195`		`Heme 2 (covalent) (By similarity).`
`BINDING`	`198 198`		`Heme 2 (covalent) (By similarity).`
`BINDING`	`244 244`		`Heme 3 (covalent) (By similarity).`
`BINDING`	`247 247`		`Heme 3 (covalent) (By similarity).`
`BINDING`	`251 251`		`Substrate (By similarity).`
`BINDING`	`301 301`		`Substrate (By similarity).`
`BINDING`	`319 319`		`Heme 4 (covalent) (By similarity).`
`BINDING`	`322 322`		`Heme 4 (covalent) (By similarity).`
`BINDING`	`353 353`		`Heme 5 (covalent) (By similarity).`
`BINDING`	`356 356`		`Heme 5 (covalent) (By similarity).`

Sequence information

Length: 525 AA [This is the length of the unprocessed precursor]

Molecular weight: 58466 Da [This is the MW of the unprocessed precursor]

CRC64: 747EF08AFB3036BB [This is a checksum on the sequence]

        10         20         30         40         50         60 
MNGVIIVNIL RKTLSSLAIV GLGFAMANSA VAEEKAMATH QQAQQLQQPA PETAAKRAPT 

        70         80         90        100        110        120 
KEELTPVNPN LKIEAANEKF AADFPRQYNS WAKTAEQTEF HKEVEDDPRM IVMWGGYAFA 

       130        140        150        160        170        180 
KEFNSPRGHI YAVTDVRNIL RTGSPKDANG GPQPMACWTC KGPDVPRLIA EWGEEGYFSG 

       190        200        210        220        230        240 
KWAKGGAEVV NSIGCADCHD TQSQDFKDGK PALRVARPHV LRALDTVGKT FATSDRTDQR 

       250        260        270        280        290        300 
AGVCANCHVE YYFDKSTGAN NVVFPWYKGR DVDSIEKYYD EIGFKDWEHS ISKAPMLKAQ 

       310        320        330        340        350        360 
HPDFETWSMG THGKNGVTCV DCHMAKTQDK DGKVYTDHQV VGNPVKDNFQ NTCARCHDQS 

       370        380        390        400        410        420 
QDTLIKTVEQ HKADVREVML KLEDQLVKSH FEAKTAWDNG ATQEEMKDAL QAIRHAQWRW 

       430        440        450        460        470        480 
DFAAASHGMH MHAPDVALKI IASGLDRVAD ARAKLAVILA KHGVQQPIQY PDISTAEKAW 

       490        500        510        520 
KVMGIDIEKE RKEKEEFIKT VIPEWNKEAI SKGLILTAPP TTPAK

Q65RI4 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools