NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=T / IAM 14863;
DOI=10.1093/nar/gkh830; PubMed=15383646 [NCBI, ExPASy, EBI, Israel, Japan]
Ueda K., Yamashita A., Ishikawa J., Shimada M., Watsuji T., Morimura K., Ikeda H., Hattori M., Beppu T.;
"Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.";
Nucleic Acids Res. 32:4937-4944(2004).

Comments

FUNCTION: Catalytic subunit of the nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC. Essential function for nitrate assimilation and may have a role in anaerobic metabolism (By similarity).
CATALYTIC ACTIVITY: Nitrite + acceptor = nitrate + reduced acceptor.
COFACTOR: Binds 1 4Fe-4S cluster (By similarity).
COFACTOR: Binds 1 molybdenum ion per subunit (By similarity).
COFACTOR: Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit (By similarity).
SUBUNIT: Interacts with napB (By similarity).
SUBCELLULAR LOCATION: Secreted (Potential). Note=Membrane-associated (Potential).
PTM: Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
SIMILARITY: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AP006840; BAD39902.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_074746.1; -.

3D structure databases

ModBase

Q67QZ1.

Enzyme and pathway databases

BioCyc

STHE292459:STH917-MON; -.

Ontologies

GO:0009055;	Molecular function: electron carrier activity (inferred from electronic annotation from HAMAP).
GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from HAMAP).
GO:0008940;	Molecular function: nitrate reductase activity (inferred from electronic annotation from HAMAP).
GO:0006777;	Biological process: Mo-molybdopterin cofactor biosynthetic process (inferred from electronic annotation from HAMAP).
GO:0042128;	Biological process: nitrate assimilation (inferred from electronic annotation from HAMAP).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01630; -; 1.
PBIL [Tree]

InterPro

IPR009010; Asp_de-COase-like_fold.
IPR006656; Mopterin_OxRdtase.
IPR006963; Mopterin_OxRdtase_Fe4S4.
IPR006655; Mopterin_OxRdtase_prok_CS.
IPR006657; MPT_dinuc_bd.
IPR006311; Tat.
Graphical view of domain structure.

Gene3D

G3DSA:2.40.40.20; Asp_decarboxylase-like_fold; 1.

Pfam

PF04879; Molybdop_Fe4S4; 1.
PF00384; Molybdopterin; 1.
PF01568; Molydop_binding; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR01409; TAT_signal_seq; 1.

PROSITE

PS00551; MOLYBDOPTERIN_PROK_1; 1.
PS00490; MOLYBDOPTERIN_PROK_2; FALSE_NEG.
PS00932; MOLYBDOPTERIN_PROK_3; FALSE_NEG.
PS51318; TAT; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q67QZ1.

Genome annotation databases

2979700; -.

AP006840_GR; STH917.

sth:STH917; -.

fig|292459.1.peg.881; -.

Phylogenomic databases

HOGENOM

Q67QZ1; -.

Genome annotation databases

CMR

Q67QZ1; STH917.

Other

ProtoNet

Q67QZ1.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

4Fe-4S; Complete proteome; Electron transport; Iron; Iron-sulfur; Metal-binding; Molybdenum; Nitrate assimilation; Oxidoreductase; Secreted; Signal; Transport.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 31`	`31`	`Tat-type signal (Potential).`
`CHAIN`	`32 754`	`723`	`Nitrate reductase.`	`PRO_0000046008`
`METAL`	`46 46`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`49 49`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`53 53`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`81 81`		`Iron-sulfur (4Fe-4S) (By similarity).`

Sequence information

Length: 754 AA [This is the length of the unprocessed precursor]

Molecular weight: 83580 Da [This is the MW of the unprocessed precursor]

CRC64: 2754BFA565A43AF0 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKFTRRSFVK ASALATAMVA AGCSPQPVAP KQNPETEGAT WYKTVCRYCG VGCGVMVAAK 

        70         80         90        100        110        120 
DNRVVAVKGD TENPVNKGLL CVKGYYLDRI MNTEEGRILK PLIRKNGQLT EASWDEALDL 

       130        140        150        160        170        180 
VAARFREAID QHGPDSVGFY GSGQNLAEET YIANKLFKGC IGTNNVEGNP RTCMASAVAG 

       190        200        210        220        230        240 
FLSTFGKDEP MGNLDDIEHA DTFFIIGSNT AEAHPIIYSR ITTRKQTGKD VKVILADPRR 

       250        260        270        280        290        300 
HRVADIADIF LPFRPGTDLA LLNAFAQVII EEGLHDPDFI ERHTTFQDGN GAITFEQYVA 

       310        320        330        340        350        360 
FLQDYTPEKV APITGLSPDD IRAAAREIGA RGRKTMTLWC MGINQRTVGT WLNNAIYNLH 

       370        380        390        400        410        420 
LLTGKICQPG NSPFSLTGQP SACGSVREGG GLSHLLPCGR QVTNEQHRKE VAAVWGVDYT 

       430        440        450        460        470        480 
RMSDKVGYHT MEMFRAAGDG RIKALLISCT NPGHSLPNLN SVRASLEKTF LVVMDAFHNR 

       490        500        510        520        530        540 
TTELADVVLP SALWCEKEGI YGNTERRTQH LAKAVEPKGE ARPDVWILLE IAKRLGYGEY 

       550        560        570        580        590        600 
FSHYTSNEVI WEEFRKMGGG GTGYDYAPYE RYKQERGLRW PVNDKQPAGT TLRYVEGDDP 

       610        620        630        640        650        660 
FVPEGAGIYF YGKPDGKAVI YARPHRDPAE VPDAEYPFYL STGRILEHWH TITMTKRVPE 

       670        680        690        700        710        720 
IMKGAGEFYC EIHEEDAARL GIQNGDLVKL TSRRGQVVAT ARVGGRAVPQ KGLVFLLMHD 

       730        740        750 
DRTERLANFL TNDAVDETSK QMEYKVCAVR VEKA

Q67QZ1 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools