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UniProtKB/Swiss-Prot entry Q69S39


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name UCRIA_ORYSJ
Primary accession number Q69S39
Secondary accession number Q9ZSU7
Integrated into Swiss-Prot on July 5, 2005
Sequence was last modified on September 13, 2004 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 34)
Name and origin of the protein
Protein name Cytochrome b6-f complex iron-sulfur subunit, chloroplastic [Precursor]
Synonyms EC 1.10.99.1
Rieske iron-sulfur protein
RISP
ISP
Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein
Gene name
Name: petC
OrderedLocusNames: Os07g0556200, LOC_Os07g37030
ORFNames: OSJNBa0058I18.25
From
Oryza sativa subsp. japonica (Rice) [TaxID: 39947] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Ilpoom;
TISSUE=Seedling leaf;
Lee J.-S.;
"Molecular cloning and characterization of cytochrome B6/F-complex iron-sulfur protein in rice.";
Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases.
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Nipponbare;
The international rice genome sequencing project (IRGSP) consortium;
"Oryza sativa nipponbare chromosome 7 genomic DNA sequence.";
Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
[3]
PROTEIN SEQUENCE OF 47-56.
STRAIN=cv. Nipponbare;
TISSUE=Leaf;
DOI=10.1093/nar/gkh020; PubMed=14681440 [NCBI, ExPASy, EBI, Israel, Japan]
Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K.;
"Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003.";
Nucleic Acids Res. 32:D388-D392(2004).
Comments
  • FUNCTION: Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions (By similarity).
  • CATALYTIC ACTIVITY: Plastoquinol-1 + 2 oxidized plastocyanin = plastoquinone + 2 reduced plastocyanin.
  • COFACTOR: Binds 1 2Fe-2S cluster per subunit (By similarity).
  • SUBUNIT: The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer (By similarity).
  • SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane; Single-pass membrane protein (By similarity). Note=The transmembrane helix obliquely spans the membrane in one monomer, and its extrinsic C-terminal domain is part of the other monomer (By similarity).
  • MISCELLANEOUS: This protein is 1 of 2 subunits of the cytochrome b6-f complex that are encoded in the nucleus.
  • MISCELLANEOUS: The Rieske iron-sulfur protein is a high potential 2Fe-2S protein.
  • SIMILARITY: Contains 1 Rieske domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF093631; AAC78103.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP005125; BAD30907.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_001059962.1; -.
UniGene Os.2248
3D structure databases
HSSP P08980; 1RFS. [HSSP ENTRY / PDB]
SMR Q69S39; 101-225.
ModBase Q69S39.
Organism-specific databases
Gramene Q69S39; -.
Ontologies
GO
GO:0009496; Molecular function: plastoquinol-plastocyanin reductase activity (inferred from electronic annotation from EC).
QuickGo view.
Family and domain databases
InterPro IPR014909; Cyt_b6-F_Fe-S_su.
IPR014349; Rieske.
IPR005805; Rieske_C.
IPR005806; Rieske_reg.
Graphical view of domain structure.
Gene3D G3DSA:2.102.10.10; Rieske_reg; 1.
PANTHER PTHR10134; Rieske; 1.
Pfam PF08802; CytB6-F_Fe-S; 1.
PF00355; Rieske; 1.
Pfam graphical view of domain structure.
PRINTS PR00162; RIESKE.
PROSITE PS51296; RIESKE; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS Q69S39.
Genome annotation databases
GeneID 4343570; -.
KEGG osa:4343570; -.
Other
ProtoNet Q69S39.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
2Fe-2S; Chloroplast; Direct protein sequencing; Electron transport; Iron; Iron-sulfur; Membrane; Metal-binding; Oxidoreductase; Plastid; Thylakoid; Transit peptide; Transmembrane; Transport.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
TRANSIT   1    46  46     Chloroplast. 
CHAIN   47   225  179     Cytochrome b6-f complex iron-sulfur subunit, chloroplastic. PRO_0000030690
TRANSMEM   69    89  21     Potential. 
DOMAIN   112   208  97     Rieske. 
METAL   154   154        Iron-sulfur (2Fe-2S) (By similarity). 
METAL   156   156        Iron-sulfur (2Fe-2S) (via pros nitrogen) (By similarity). 
METAL   172   172        Iron-sulfur (2Fe-2S) (By similarity). 
METAL   175   175        Iron-sulfur (2Fe-2S) (via pros nitrogen) (By similarity). 
DISULFID   159   174        By similarity. 
Sequence information
Length: 225 AA [This is the length of the unprocessed precursor] Molecular weight: 23884 Da [This is the MW of the unprocessed precursor] CRC64: 3CB5116F297AF8B0 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MASTALSTAS NPTQLCRSRA SLGKPVKGLG FGRERVPRTA TTITCQAASS IPADRVPDMG 

        70         80         90        100        110        120 
KRQLMNLLLL GAISLPTVGM LVPYGAFFIP AGSGNAGGGQ VAKDKLGNDV LAEEWLKTHG 

       130        140        150        160        170        180 
PNDRTLTQGL KGDPTYLVVE ADKTLATYGI NAVCTHLGCV VPWNAAENKF ICPCHGSQYN 

       190        200        210        220 
NQGRVVRGPA PLSLALVHAD VDDGKVLFVP WVETDFRTGD NPWWA 

Q69S39 in FASTA format

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