FUNCTION: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism (By similarity).
CATALYTIC ACTIVITY: Nitrite + acceptor = nitrate + reduced acceptor.
COFACTOR: Binds 1 4Fe-4S cluster (By similarity).
COFACTOR: Binds 1 molybdenum ion per subunit (By similarity).
COFACTOR: Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit (By similarity).
SUBUNIT: Interacts with napB (By similarity).
SUBCELLULAR LOCATION: Periplasm (By similarity).
PTM: Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
SIMILARITY: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BX950851; CAG74799.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_049993.1; -.

3D structure databases

SMR

Q6D5Z2; 37-827.

ModBase

Q6D5Z2.

Enzyme and pathway databases

BioCyc

ECAR218491:ECA1896-MON; -.

Ontologies

GO:0009055;	Molecular function: electron carrier activity (inferred from electronic annotation from HAMAP).
GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from HAMAP).
GO:0008940;	Molecular function: nitrate reductase activity (inferred from electronic annotation from HAMAP).
GO:0006777;	Biological process: Mo-molybdopterin cofactor biosynthetic process (inferred from electronic annotation from HAMAP).
GO:0042128;	Biological process: nitrate assimilation (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01630; -; 1.
PBIL [Tree]

InterPro

IPR009010; Asp_de-COase-like_fold.
IPR006656; Mopterin_OxRdtase.
IPR006963; Mopterin_OxRdtase_Fe4S4.
IPR006655; Mopterin_OxRdtase_prok_CS.
IPR006657; MPT_dinuc_bd.
IPR010051; NO3_reductase_lsu_periplasm.
IPR006311; Tat.
Graphical view of domain structure.

Gene3D

G3DSA:2.40.40.20; Asp_decarboxylase-like_fold; 1.

Pfam

PF04879; Molybdop_Fe4S4; 1.
PF00384; Molybdopterin; 1.
PF01568; Molydop_binding; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR01706; NAPA; 1.
TIGR01409; TAT_signal_seq; 1.

PROSITE

PS00551; MOLYBDOPTERIN_PROK_1; 1.
PS00490; MOLYBDOPTERIN_PROK_2; FALSE_NEG.
PS00932; MOLYBDOPTERIN_PROK_3; FALSE_NEG.
PS51318; TAT; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q6D5Z2.

Genome annotation databases

GeneID

2884039; -.

GenomeReviews

BX950851_GR; ECA1896.

KEGG

eca:ECA1896; -.

NMPDR

fig|218491.3.peg.696; -.

Phylogenomic databases

HOGENOM

Q6D5Z2; -.

Genome annotation databases

CMR

Q6D5Z2; ECA1896.

Other

ProtoNet

Q6D5Z2.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

4Fe-4S; Complete proteome; Electron transport; Iron; Iron-sulfur; Metal-binding; Molybdenum; Nitrate assimilation; Oxidoreductase; Periplasm; Signal; Transport.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 31`	`31`	`Tat-type signal (Potential).`
`CHAIN`	`32 828`	`797`	`Periplasmic nitrate reductase.`	`PRO_0000045984`
`METAL`	`46 46`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`49 49`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`53 53`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`81 81`		`Iron-sulfur (4Fe-4S) (By similarity).`

Sequence information

Length: 828 AA [This is the length of the unprocessed precursor]

Molecular weight: 92780 Da [This is the MW of the unprocessed precursor]

CRC64: 2F43B05328AD7A46 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKLSRRHFMK ANAVAAAAAV AGITIPIAVR AATEQSDAIH WDKAPCRFCG VGCGVLVGTQ 

        70         80         90        100        110        120 
NGRIVASQGD PEAPVNRGLN CIKGYFLPKI MYGQDRLTQP LLRMRNGQFD KEGEFTPISW 

       130        140        150        160        170        180 
DKAFDIMAEK FKTALKEKGP NAIGMFGSGQ STIWEGYASA KLFKAGFRSN NIDPNARHCM 

       190        200        210        220        230        240 
ASAVVGFMRT FGMDEPMGCY DDIEQTDAFV LWGSNMAEMH PILWSRITDR RLSNSNVTVA 

       250        260        270        280        290        300 
VLSTYQHRSF ELADNGMVFT PQTDLAILNY IANYIIQNNA VNEAFFTRHV NLRRGVTDIG 

       310        320        330        340        350        360 
YGLRPTHPLE KAAKNPGSDA SEPMSFEEYK AFVADYTLEK TVAISGVPAD QLEALAKLYA 

       370        380        390        400        410        420 
DPKKKVISYW TMGFNQHTRG VWANNLVYNI HLLTGKISQP GCGPFSLTGQ PSACGTAREV 

       430        440        450        460        470        480 
GTFAHRLPAD MVVTNEKHRA IAEKLWQLPT GTIPEKIGLH AVAQDRALKD GTLNAYWVMC 

       490        500        510        520        530        540 
NNNMQAGPNI NQERMPGWRD PRNFIVVSDP YPTISALAAD LILPTAMWVE KEGAYGNAER 

       550        560        570        580        590        600 
RTQFWRQQVK APGESKSDLW QVVSFAKRFT VEDVWPEELL AQKPAYRGKT LYDVLFANDV 

       610        620        630        640        650        660 
TTRFPLSELA ENQLNDESRE FGFYLQKGLF EEYAAFGRGH GHDLAPFDAY HKVRGLRWPV 

       670        680        690        700        710        720 
VDGKETQWRY SEGHDPYVKA GEAYRFYGKP DGKAVIFALP YEPAAEAPDE EYDLWFSTGR 

       730        740        750        760        770        780 
VLEHWHTGSM TRRVPELHRA FPEAVLFIHP QDAKARDLRR GEKVRIISRR GEVISVVETR 

       790        800        810        820 
GRNKPPRGLV YMPFFDAAQM TNVLTLDATD PLSKETDFKK CAVKLAKV

Q6D5Z2 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools