[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. TISSUE=Embryo; NIH - Xenopus Gene Collection (XGC) project; Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a substrate for HOT when using 2-KG as hydrogen acceptor, resulting in the formation of D-2-HG (By similarity).
CATALYTIC ACTIVITY: (S)-3-hydroxybutanoate + 2-oxoglutarate = acetoacetate + (R)-2-hydroxyglutarate.
CATALYTIC ACTIVITY: 2-oxoglutaric acid + 4-hydroxybutanoic acid = (R)-2-hydroxyglutaric acid + succinic semialdehyde.
SUBCELLULAR LOCATION: Mitochondrion (By similarity).
SIMILARITY: Belongs to the iron-containing alcohol dehydrogenase family. Hydroxyacid-oxoacid transhydrogenase subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BC064162; AAH64162.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_989277.1; -.

UniGene

Str.5492

3D structure databases

ModBase

Q6P371.

Ontologies

GO:0005739;	Cellular component: mitochondrion (inferred from sequence or structural similarity from UniProtKB).
GO:0047988;	Molecular function: hydroxyacid-oxoacid transhydrogenase activity (inferred from sequence or structural similarity from UniProtKB).
GO:0015993;	Biological process: molecular hydrogen transport (inferred from sequence or structural similarity from UniProtKB).
QuickGo view.

Family and domain databases

InterPro

IPR001670; Fe_AlcDHase.
Graphical view of domain structure.

Pfam

PF00465; Fe-ADH; 1.
Pfam graphical view of domain structure.

PROSITE

PS00913; ADH_IRON_1; FALSE_NEG.
PS00060; ADH_IRON_2; FALSE_NEG.

BLOCKS

Q6P371.

Genome annotation databases

Ensembl

ENSXETG00000023251; Xenopus tropicalis. [Contig view]

GeneID

394891; -.

KEGG

xtr:394891; -.

Phylogenomic databases

HOGENOM

Q6P371; -.

HOVERGEN

Q6P371; -.

Other

ProtoNet

Q6P371.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Mitochondrion; Oxidoreductase; Transit peptide.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 ?`		`Mitochondrion (Potential).`
`CHAIN`	`? 463`		`Hydroxyacid-oxoacid transhydrogenase, mitochondrial.`	`PRO_0000323001`

Sequence information

Length: 463 AA [This is the length of the unprocessed precursor]

Molecular weight: 50408 Da [This is the MW of the unprocessed precursor]

CRC64: F6CD5F2B90C513C1 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAARRDQVIH LLRQLQRASC KCPAHSHTYS QAPVTGRKTE YAFEMAVSNI RYGENVTQEI 

        70         80         90        100        110        120 
GMDLQNLGAR NVCVMTDRNL VELSPVKAVL NSLVKNNVSF KLYDRVRVEP TDKSFMDAIE 

       130        140        150        160        170        180 
FAKKGQFDAY VGVGGGSVID TCKAANLYSS SPGADFLDYV NPPIGKGKAV TVPLKPLIAV 

       190        200        210        220        230        240 
PTTSGTGSET TGIAIFDYEE LKAKTGIASR AIKPTLGLID PVHTLSMPER VVANSGFDVL 

       250        260        270        280        290        300 
CHSLESYTAL PYNMRSPCPT NPINRPAYQG SNPISDVWAK HALRIVAKYL KRAVRNPDDR 

       310        320        330        340        350        360 
EARFAMHLAS SFAGVGFGNA GVHLCHGMSY PIAGHVKTYR AKDYEVDHPL VPHGLSVVLT 

       370        380        390        400        410        420 
SPAVFSFTAL MCPERHLEAA EILGADIRTA KIKEAGLILA DTLRKFLYDL NVDDGLAAVG 

       430        440        450        460 
YTTEDIPALV KGTLPQERVT KLSPRAHSEE ELAGLFEASM KLY

Q6P371 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools