NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC BAA-968 / K-10;
DOI=10.1073/pnas.0505662102; PubMed=16116077 [NCBI, ExPASy, EBI, Israel, Japan]
Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V.;
"The complete genome sequence of Mycobacterium avium subspecies paratuberculosis.";
Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005).

Comments

FUNCTION: Flavoprotein monooxygenase required for N-hydroxylation of the two acylated lysine residues during mycobactin assembly, thus producing the hydroxamate groups necessary for iron sequestration. Is also able, but less efficiently, to hydroxylate L-lysine (non acylated) in vitro (By similarity).
CATALYTIC ACTIVITY: L-lysine + NADPH + O₂ = N⁶-hydroxy-L-lysine + NADP⁺ + H₂O.
COFACTOR: FAD (By similarity).
PATHWAY: Siderophore biosynthesis; mycobactin biosynthesis .
SIMILARITY: Belongs to the lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AE016958; AAS04487.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_961104.1; -.

3D structure databases

ModBase

Q73XY8.

Family and domain databases

BLOCKS

Q73XY8.

Genome annotation databases

GeneID

2719638; -.

GenomeReviews

AE016958_GR; MAP_2170c.

KEGG

mpa:MAP2170c; -.

NMPDR

fig|262316.1.peg.2170; -.

Phylogenomic databases

HOGENOM

Q73XY8; -.

Genome annotation databases

CMR

Q73XY8; MAP_2170c.

Other

ProtoNet

Q73XY8.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; FAD; Flavoprotein; Ion transport; Iron; Iron transport; Monooxygenase; NADP; Oxidoreductase; Signal; Transport.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 20`	`20`	`Potential.`
`CHAIN`	`21 428`	`408`	`L-lysine 6-monooxygenase mbtG.`	`PRO_0000261310`

Sequence information

Length: 428 AA [This is the length of the unprocessed precursor]

Molecular weight: 46532 Da [This is the MW of the unprocessed precursor]

CRC64: 44C8EE39A4AFA801 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSTLAILGAG AKAVAVAAKA SVLRDMGVEV PDVVAVERIG VAANWQASGG WTDGAHRLGT 

        70         80         90        100        110        120 
SPEKDVGFPY RSALVPRRNA ELDERMTRYS WQSYLIATAS FAEWIDRGRP APTHRRWSQY 

       130        140        150        160        170        180 
LSWVADHVGM TVVHGEVEQL AVTGDRWALH THETTVHADA LMITGPGQAE KSLLPGNPRM 

       190        200        210        220        230        240 
LSIAQFWDRA ANHDRISAER VAVIGGGETA AAMLNELFRH RVSSITVISP QATLFTRGEG 

       250        260        270        280        290        300 
YFENSLFSDP TNWPALTLAE RRDALARTDR GVFSSSVQEA LLADDRIHHL RGRVTHAVGV 

       310        320        330        340        350        360 
QGQIRLTLST NRGSENLETV HGFDLVIDGS GADSLWFAPL FSQEALDLLE LGLGGPLSSE 

       370        380        390        400        410        420 
RLQEAIGYDL AVTDVTPKLF LPNLSGLTQG PGFPNLSCLG LLSDRVLGST LGPTNYPARR 


RHDERQPL

Q73XY8 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools