ID   HCAE_PHOLL              Reviewed;         453 AA.
AC   Q7N4W0;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   25-NOV-2008, entry version 33.
DE   RecName: Full=3-phenylpropionate/cinnamic acid dioxygenase subunit alpha;
DE            EC=1.14.12.19;
DE   AltName: Full=Digoxigenin subunit alpha;
GN   Name=hcaE; OrderedLocusNames=plu2204;
OS   Photorhabdus luminescens subsp. laumondii.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Photorhabdus.
OX   NCBI_TaxID=141679;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TT01;
RX   MEDLINE=22957627; PubMed=14528314; DOI=10.1038/nbt886;
RA   Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A.,
RA   Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F.,
RA   Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S.,
RA   Medigue C., Lanois A., Powell K., Siguier P., Vincent R., Wingate V.,
RA   Zouine M., Glaser P., Boemare N., Danchin A., Kunst F.;
RT   "The genome sequence of the entomopathogenic bacterium Photorhabdus
RT   luminescens.";
RL   Nat. Biotechnol. 21:1307-1313(2003).
CC   -!- FUNCTION: Part of the multicomponent 3-phenylpropionate
CC       dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic
CC       acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and
CC       cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: 3-phenylpropanoic acid + NADH + O(2) = cis-3-
CC       (2-carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD(+).
CC   -!- CATALYTIC ACTIVITY: Cinnamic acid + H(+) + NADH + O(2) = cis-3-(2-
CC       carboxyethenyl)-3,5-cyclohexadiene-1,2-diol + NAD(+).
CC   -!- COFACTOR: Binds 1 iron ion (By similarity).
CC   -!- COFACTOR: Binds 1 2Fe-2S cluster per subunit (By similarity).
CC   -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropionic acid
CC       degradation.
CC   -!- SUBUNIT: This dioxygenase system consists of four proteins: the
CC       two subunits of the hydroxylase component (hcaE and hcaF), a
CC       ferredoxin (hcaC) and a ferredoxin reductase (hcaD) (By
CC       similarity).
CC   -!- SIMILARITY: Belongs to the bacterial ring-hydroxylating
CC       dioxygenase alpha subunit family.
CC   -!- SIMILARITY: Contains 1 Rieske domain.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; BX571866; CAE14497.1; -; Genomic_DNA.
DR   RefSeq; NP_929462.1; -.
DR   HSSP; P23094; 1EG9.
DR   GeneID; 2802209; -.
DR   GenomeReviews; BX470251_GR; plu2204.
DR   KEGG; plu:plu2204; -.
DR   PhotoList; plu2204; -.
DR   HOGENOM; Q7N4W0; -.
DR   BioCyc; PLUM243265:PLU2204-MON; -.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:InterPro.
DR   GO; GO:0008695; F:3-phenylpropionate dioxygenase activity; IEA:HAMAP.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:HAMAP.
DR   GO; GO:0016702; F:oxidoreductase activity, acting on single d...; IEA:UniProtKB-KW.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:InterPro.
DR   HAMAP; MF_01648; -; 1.
DR   InterPro; IPR005806; Rieske_reg.
DR   InterPro; IPR015879; Ring_hydroxy_dOase_asu_C.
DR   InterPro; IPR001663; Rng_hydr_dOase-A.
DR   Gene3D; G3DSA:2.102.10.10; Rieske_reg; 1.
DR   PANTHER; PTHR21266:SF2; Rng_hydr_dOase-A; 1.
DR   Pfam; PF00355; Rieske; 1.
DR   Pfam; PF00848; Ring_hydroxyl_A; 1.
DR   PRINTS; PR00090; RNGDIOXGNASE.
DR   PROSITE; PS51296; RIESKE; 1.
DR   PROSITE; PS00570; RING_HYDROXYL_ALPHA; FALSE_NEG.
PE   3: Inferred from homology;
KW   2Fe-2S; Aromatic hydrocarbons catabolism; Complete proteome;
KW   Dioxygenase; Iron; Iron-sulfur; Metal-binding; NAD; Oxidoreductase.
FT   CHAIN         1    453       3-phenylpropionate/cinnamic acid
FT                                dioxygenase subunit alpha.
FT                                /FTId=PRO_0000333705.
FT   DOMAIN       43    141       Rieske.
FT   METAL        85     85       Iron-sulfur (2Fe-2S) (By similarity).
FT   METAL        87     87       Iron-sulfur (2Fe-2S); via pros nitrogen
FT                                (By similarity).
FT   METAL       105    105       Iron-sulfur (2Fe-2S) (By similarity).
FT   METAL       108    108       Iron-sulfur (2Fe-2S); via pros nitrogen
FT                                (By similarity).
FT   METAL       213    213       Iron (By similarity).
FT   METAL       218    218       Iron (By similarity).
SQ   SEQUENCE   453 AA;  51322 MW;  150ECAD0216477AE CRC64;
     MTLSKDSDIY RLIDARAGRV TPQIYIDPEL YQLELERIFG RCWLFLAHQS QIPNPGDFFN
     TYMGEDSVVV VRQKNGSVKA FLNQCRHRSM RVCYADSGNT RAFTCPYHGW SYGVDGRLID
     VPLEACAYPH GLCKEQWGLQ EVPCVENYKG LIFGNWDTTA PSLIDYLGDM AWYLDGVLDR
     REGGTEVIGG VQKWLINCNW KLPAEQFAGD QYHALFSHAS AVQVLSVKDG DDKKALGADQ
     TSRPVWETAK DAVQFAQNGH GCGFFLTEKP DANVWVDGAV ARYYRETYAE AEQRLGKVRA
     LRLAGHNNIF PTLSWLNGTA TMRVWHPRGP DQVEVWAFCI ADKAASPEVK AAFENSATRA
     FGPAGFLEQD DSENWVEIQK VLRGYKARNS TLCMEMRLGQ ERVRDDGIPG VTNYVFSETV
     ARGMYQRWAD LLTSETWDEI EEKSRVYQQE LVK
//