ID   CYSI_PHOLL              Reviewed;         576 AA.
AC   Q7N8L5;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   25-NOV-2008, entry version 30.
DE   RecName: Full=Sulfite reductase [NADPH] hemoprotein beta-component;
DE            Short=SIR-HP;
DE            Short=SIRHP;
DE            EC=1.8.1.2;
GN   Name=cysI; OrderedLocusNames=plu0704;
OS   Photorhabdus luminescens subsp. laumondii.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Photorhabdus.
OX   NCBI_TaxID=141679;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TT01;
RX   MEDLINE=22957627; PubMed=14528314; DOI=10.1038/nbt886;
RA   Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A.,
RA   Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F.,
RA   Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S.,
RA   Medigue C., Lanois A., Powell K., Siguier P., Vincent R., Wingate V.,
RA   Zouine M., Glaser P., Boemare N., Danchin A., Kunst F.;
RT   "The genome sequence of the entomopathogenic bacterium Photorhabdus
RT   luminescens.";
RL   Nat. Biotechnol. 21:1307-1313(2003).
CC   -!- FUNCTION: This enzyme catalyzes the 6-electron reduction of
CC       sulfite to sulfide. This is one of several activities required for
CC       the biosynthesis of L-cysteine from sulfate (By similarity).
CC   -!- CATALYTIC ACTIVITY: H(2)S + 3 NADP(+) + 3 H(2)O = sulfite + 3
CC       NADPH.
CC   -!- COFACTOR: Binds 1 siroheme per subunit (By similarity).
CC   -!- COFACTOR: Binds 1 4Fe-4S cluster per subunit (By similarity).
CC   -!- SUBUNIT: Alpha(8)-beta(4). The alpha component is a flavoprotein,
CC       the beta component is a hemoprotein (By similarity).
CC   -!- SIMILARITY: Belongs to the nitrite and sulfite reductase 4Fe-4S
CC       domain family.
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DR   EMBL; BX571861; CAE12999.1; -; Genomic_DNA.
DR   RefSeq; NP_928049.1; -.
DR   HSSP; P17846; 1AOP.
DR   SMR; Q7N8L5; 81-571.
DR   GeneID; 2800667; -.
DR   GenomeReviews; BX470251_GR; plu0704.
DR   KEGG; plu:plu0704; -.
DR   NMPDR; fig|243265.1.peg.673; -.
DR   PhotoList; plu0704; -.
DR   HOGENOM; Q7N8L5; -.
DR   BioCyc; PLUM243265:PLU0704-MON; -.
DR   GO; GO:0009337; C:sulfite reductase complex (NADPH); IEA:InterPro.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:InterPro.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR   GO; GO:0004783; F:sulfite reductase (NADPH) activity; IEA:HAMAP.
DR   GO; GO:0019344; P:cysteine biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0000103; P:sulfate assimilation; IEA:HAMAP.
DR   HAMAP; MF_01540; -; 1.
DR   InterPro; IPR011786; CysI.
DR   InterPro; IPR006066; Nir_Si_BS.
DR   InterPro; IPR006067; Nir_Sir_4Fe4S.
DR   InterPro; IPR005117; NiRdtase/SiRdtase_haem-b_fer.
DR   Pfam; PF01077; NIR_SIR; 1.
DR   Pfam; PF03460; NIR_SIR_ferr; 2.
DR   PRINTS; PR00397; SIROHAEM.
DR   TIGRFAMs; TIGR02041; CysI; 1.
DR   PROSITE; PS00365; NIR_SIR; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Amino-acid biosynthesis; Complete proteome;
KW   Cysteine biosynthesis; Heme; Iron; Iron-sulfur; Metal-binding; NADP;
KW   Oxidoreductase.
FT   CHAIN         1    576       Sulfite reductase [NADPH] hemoprotein
FT                                beta-component.
FT                                /FTId=PRO_0000199903.
FT   METAL       435    435       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL       441    441       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL       480    480       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL       484    484       Iron (siroheme axial ligand) (By
FT                                similarity).
FT   METAL       484    484       Iron-sulfur (4Fe-4S) (By similarity).
SQ   SEQUENCE   576 AA;  64829 MW;  3CA6B1166715B15D CRC64;
     MNDKQHGPFI VEGKLADSER MKQESNFLRG TISEDLTNGL TGGFEGDNFL LIRFHGMYQQ
     DDRDIRAERA EQKLEPRHAI MLRCRLPGGV ITPQQWLGID KFAEENTLYG SIRLTNRQTF
     QFHGILKGKV KLAHQLLNQV GLDSLATAND VNRNVLCTSN PIQSELHQQA YEWAKKISEH
     LLPRTRAYAE LWLDKEKVAT TDEEPILGST YLPRKFKTTV VIPPQNDVDL HANDLNFIAI
     AEGSKLVGFN VLVGGGLAMT HGDKNTYPSM ASEFGYIPLE HTLAIAEAVV TTQRDWGNRT
     ERKNAKTKYT LERVGVETFK AEVERRAGVK FEAIRPYEFT GRGDQIGWLK GIDDKWHLTL
     FIENGRLLDY PGKPLKSGVA EIAKIHKGDF RLTANQNLII AGIPESEKQR IETIACRHGL
     IDDKTTVQRK NSMACVSFPT CPLAMAEAER FLPEFVTHVE QLMNKHGIGD EHIVLRVTGC
     PNGCARAMLA EVGLVGKALD RYNLHLGGNR IGTRIPRMYK ENISSQAILS IMDELIGRWA
     TGRQPNEGFG DFLIRTDIIK PVLDSARDFY DWQEAV
//