ID   ACSF_GLOVI              Reviewed;         346 AA.
AC   Q7NFA1;
DT   16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   25-NOV-2008, entry version 38.
DE   RecName: Full=Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase;
DE            Short=Mg-protoporphyrin IX monomethyl ester oxidative cyclase;
DE            EC=1.14.13.81;
GN   Name=acsF; OrderedLocusNames=gll3625;
OS   Gloeobacter violaceus.
OC   Bacteria; Cyanobacteria; Gloeobacteria; Gloeobacterales; Gloeobacter.
OX   NCBI_TaxID=33072;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 7421;
RX   MEDLINE=22977040; PubMed=14621292; DOI=10.1093/dnares/10.4.137;
RA   Nakamura Y., Kaneko T., Sato S., Mimuro M., Miyashita H., Tsuchiya T.,
RA   Sasamoto S., Watanabe A., Kawashima K., Kishida Y., Kiyokawa C.,
RA   Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S.,
RA   Takeuchi C., Yamada M., Tabata S.;
RT   "Complete genome structure of Gloeobacter violaceus PCC 7421, a
RT   cyanobacterium that lacks thylakoids.";
RL   DNA Res. 10:137-145(2003).
CC   -!- FUNCTION: Catalyzes the formation of the isocyclic ring in
CC       chlorophyll biosynthesis. Mediates the cyclase reaction, which
CC       results in the formation of divinylprotochlorophyllide (Pchlide)
CC       characteristic of all chlorophylls from magnesium-protoporphyrin
CC       IX 13-monomethyl ester (MgPMME) (By similarity).
CC   -!- CATALYTIC ACTIVITY: Magnesium-protoporphyrin IX 13-monomethyl
CC       ester + NADPH + O(2) = 13(1)-hydroxy-magnesium-protoporphyrin IX
CC       13-monomethyl ester + NADP(+) + H(2)O.
CC   -!- CATALYTIC ACTIVITY: 13(1)-hydroxy-magnesium-protoporphyrin IX 13-
CC       monomethyl ester + NADPH + O(2) = 13(1)-oxo-magnesium-
CC       protoporphyrin IX 13-monomethyl ester + NADP(+) + 2 H(2)O.
CC   -!- CATALYTIC ACTIVITY: 13(1)-oxo-magnesium-protoporphyrin IX 13-
CC       monomethyl ester + NADPH + O(2) = divinylprotochlorophyllide +
CC       NADP(+) + 2 H(2)O.
CC   -!- COFACTOR: Iron (By similarity).
CC   -!- PATHWAY: Porphyrin biosynthesis; chlorophyll biosynthesis (light-
CC       independent).
CC   -!- SIMILARITY: Belongs to the acsF family.
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DR   EMBL; BA000045; BAC91566.1; -; Genomic_DNA.
DR   RefSeq; NP_926571.1; -.
DR   GeneID; 2602029; -.
DR   GenomeReviews; BA000045_GR; gll3625.
DR   KEGG; gvi:gll3625; -.
DR   NMPDR; fig|251221.1.peg.3625; -.
DR   HOGENOM; Q7NFA1; -.
DR   BioCyc; GVIO251221:GLL3625-MON; -.
DR   GO; GO:0005506; F:iron ion binding; IEA:HAMAP.
DR   GO; GO:0048529; F:magnesium-protoporphyrin IX monomethyl este...; IEA:HAMAP.
DR   GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0015979; P:photosynthesis; IEA:HAMAP.
DR   HAMAP; MF_01840; -; 1.
DR   InterPro; IPR008434; AcsF.
DR   InterPro; IPR003251; Rubrerythrin.
DR   Pfam; PF02915; Rubrerythrin; 1.
DR   TIGRFAMs; TIGR02029; AcsF; 1.
PE   3: Inferred from homology;
KW   Chlorophyll biosynthesis; Complete proteome; Iron; Metal-binding;
KW   NADP; Oxidoreductase; Photosynthesis.
FT   CHAIN         1    346       Magnesium-protoporphyrin IX monomethyl
FT                                ester [oxidative] cyclase.
FT                                /FTId=PRO_0000217529.
SQ   SEQUENCE   346 AA;  40171 MW;  60FA7605D5EB8749 CRC64;
     MVDALREANT ASQSERRETL LTPRFYTTDF EALAKLDISA MEADFEALLA EFQADYNREH
     FQRDAGFVEQ AHQIDPQWRA LMLEFLERSC TSEFSGFLLY KEISRRLKAA NPLLAECFKA
     MSRDEARHAG FLNKAMGDYD LGLDLSNLAK QKKYTFFKPK FIFYATYLSE KIGYWRYIAI
     FRHLERHPEN RIHPLFNYFK NWCQDENRHG DFFGLILKSQ PQLLKGPINR LWVRFFLLSF
     CLTMYLNDRK PHNLYSALGL DASHYAMEVL RQTNLDSGKV FPLILDLDNP RFQRHLDRCA
     DLNLQLEALS GKSPLARLPV IAALAGTIAQ LFLLKPVAPP APEMVR
//