NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION BY MASS SPECTROMETRY, ENZYME ACTIVITY, AND SUBUNIT.
TISSUE=Heart;
DOI=10.1074/jbc.M302851200; PubMed=12654921 [NCBI, ExPASy, EBI, Israel, Japan]
Miinalainen I.J., Chen Z.-J., Torkko J.M., Pirilae P.L., Sormunen R.T., Bergmann U., Qin Y.-M., Hiltunen J.K.;
"Characterization of 2-enoyl thioester reductase from mammals: an ortholog of Ybr026p/Mrf1'p of the yeast mitochondrial fatty acid synthesis type II.";
J. Biol. Chem. 278:20154-20161(2003).

Comments

FUNCTION: Catalyzes the reduction of trans-2-enoyl-CoA to acyl-CoA with chain length from C6 to C16 in an NADPH-dependent manner with preference to medium chain length substrate. May have a role in the mitochondrial synthesis of fatty acids.
CATALYTIC ACTIVITY: Acyl-CoA + NADP⁺ = trans-2,3-dehydroacyl-CoA + NADPH.
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Mitochondrion (By similarity).
SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AY256973; AAP45003.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_858055.1; -.

UniGene

Bt.18851

3D structure databases

HSSP

Q8WZM4; 1H0K. [HSSP ENTRY / PDB]

SMR

Q7YS70; 40-373.

ModBase

Q7YS70.

Ontologies

GO:0005739;	Cellular component: mitochondrion (inferred from sequence or structural similarity from UniProtKB).
GO:0019166;	Molecular function: trans-2-enoyl-CoA reductase (NADPH) activity (inferred from direct assay from UniProtKB).
GO:0006631;	Biological process: fatty acid metabolic process (inferred from direct assay from UniProtKB).
QuickGo view.

Family and domain databases

InterPro

IPR013154; AlcDHase_GroES-like.
IPR002085; AlcDHase_SF_Zn.
IPR013149; AlcDHase_Zn-bd.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

PANTHER

PTHR11695; ADH_Sf_Zn; 1.

Pfam

PF08240; ADH_N; 1.
PF00107; ADH_zinc_N; 1.
Pfam graphical view of domain structure.

BLOCKS

Q7YS70.

Genome annotation databases

Ensembl

ENSBTAG00000017253; Bos taurus. [Contig view]

GeneID

353301; -.

KEGG

bta:353301; -.

Phylogenomic databases

HOVERGEN

Q7YS70; -.

Other

ProtoNet

Q7YS70.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Fatty acid biosynthesis; Lipid synthesis; Mitochondrion; NADP; Oxidoreductase; Transit peptide.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 53`	`53`	`Mitochondrion (Potential).`
`CHAIN`	`54 373`	`320`	`Trans-2-enoyl-CoA reductase, mitochondrial.`	`PRO_0000000887`

Sequence information

Length: 373 AA [This is the length of the unprocessed precursor]

Molecular weight: 40275 Da [This is the MW of the unprocessed precursor]

CRC64: 7921122C7735A95D [This is a checksum on the sequence]

        10         20         30         40         50         60 
MWVCGALCRT RAPAQLGQRL LPESRRRRPA SASFSASAEP SRVRALVYGH HGDPAKVVEL 

        70         80         90        100        110        120 
KNLELAAVGG SHVHVKMLAA PINPSDINMI QGNYGLLPQL PAVGGNEGVG QVVAVGSGVT 

       130        140        150        160        170        180 
GVKPGDWVIP ANPGLGTWRT EAVFGEEELI TVPSDIPLQS AATLGVNPCT AYRMLVDFER 

       190        200        210        220        230        240 
LRPRDSIIQN ASNSGVGQAV IQIAAARGLR TINVLRDTPD LQKLTDTLKN LGANHVVTEE 

       250        260        270        280        290        300 
ELRKPEMKSF FKDVPQPRLA LNCVGGKSST ELLRHLAPGG TMVTYGGMAK QPVIASVSQL 

       310        320        330        340        350        360 
IFKDLKLRGF WLSQWKKDHS PDQFKELILT LCDLIRRGQL TAPACSEVPL QDYLCALEAS 

       370 
TQPFVSSKQI LTM

Q7YS70 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools