FUNCTION: Catalyzes the reduction of the double bond of an array of alpha, beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes (By similarity).
CATALYTIC ACTIVITY: NADPH + acceptor = NADP⁺ + reduced acceptor.
COFACTOR: FMN (By similarity).
SUBUNIT: Homotetramer (By similarity).
SIMILARITY: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. NamA subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AE016877; AAP08992.1; ALT_INIT; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_831791.1; -.

3D structure databases

HSSP

Q51990; 1GWJ. [HSSP ENTRY / PDB]

ModBase

Q81EF6.

Enzyme and pathway databases

BioCyc

BCER226900:BC_2023-MON; -.

Ontologies

GO:0003959;	Molecular function: NADPH dehydrogenase activity (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01614; -; 1.
PBIL [Tree]

InterPro

IPR013785; Aldolase_TIM.
IPR001155; OxRdtase_FMN_N.
Graphical view of domain structure.

Gene3D

G3DSA:3.20.20.70; Aldolase_TIM; 1.

Pfam

PF00724; Oxidored_FMN; 1.
Pfam graphical view of domain structure.

BLOCKS

Q81EF6.

Genome annotation databases

GeneID

1204372; -.

GenomeReviews

AE016877_GR; BC_2023.

KEGG

bce:BC2023; -.

Phylogenomic databases

HOGENOM

Q81EF6; -.

Genome annotation databases

CMR

Q81EF6; BC_2023.

Other

ProtoNet

Q81EF6.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Flavoprotein; FMN; NADP; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 345`	`345`	`NADPH dehydrogenase.`	`PRO_0000216114`
`NP_BIND`	`23 27`	`5`	`FMN (By similarity).`
`BINDING`	`28 28`		`Substrate (By similarity).`
`BINDING`	`164 164`		`Substrate (By similarity).`
`BINDING`	`167 167`		`Substrate (By similarity).`
`BINDING`	`215 215`		`FMN (By similarity).`
`BINDING`	`308 308`		`FMN (By similarity).`

Sequence information

Length: 345 AA [This is the length of the unprocessed precursor]

Molecular weight: 38582 Da [This is the MW of the unprocessed precursor]

CRC64: 77A2907FB25678FD [This is a checksum on the sequence]

        10         20         30         40         50         60 
MNSKLFSPYT IKNVTLKNRI VMSPMCMYSS GNEDGRVTNF HLIHYGTRAA GQVGLVMVEA 

        70         80         90        100        110        120 
TAVLAEGRIS NNDLGIWDDN LIEGLHKTTT FIHDNGAKAA IQLAHAGRKA ELDTNAFAPS 

       130        140        150        160        170        180 
AIPFNDKMKI PVEMNIQQIK ETILAFQRAA LRSKQAGFDV IELHGAHGYL INEFLSPLTN 

       190        200        210        220        230        240 
KRTDKYGGSP ENRYRFLREI IDSVNEVWDG PIFVRISAND YHPDGLTVQD YVQYTKWMKE 

       250        260        270        280        290        300 
QGIDLIDCSS GAVVPAHIDV YPGYQVQYAK HIKEHTNIAT GAVGLITTGS QAEQILNNNE 

       310        320        330        340 
ADLIFIGREL LRNPYFPRIA ANELGFELQE PHQYKRAPGK IHTNK

Q81EF6 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools