ID   MSOX_STRAW              Reviewed;         384 AA.
AC   Q827H4;
DT   04-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   04-NOV-2008, entry version 39.
DE   RecName: Full=Monomeric sarcosine oxidase;
DE            Short=MSOX;
DE            EC=1.5.3.1;
GN   Name=soxA; Synonyms=solA; OrderedLocusNames=SAV_6950;
OS   Streptomyces avermitilis.
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Streptomycineae; Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=33903;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165;
RX   MEDLINE=21477403; PubMed=11572948; DOI=10.1073/pnas.211433198;
RA   Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C.,
RA   Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T.,
RA   Kikuchi H., Shiba T., Sakaki Y., Hattori M.;
RT   "Genome sequence of an industrial microorganism Streptomyces
RT   avermitilis: deducing the ability of producing secondary
RT   metabolites.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165;
RX   MEDLINE=22608306; PubMed=12692562; DOI=10.1038/nbt820;
RA   Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T.,
RA   Sakaki Y., Hattori M., Omura S.;
RT   "Complete genome sequence and comparative analysis of the industrial
RT   microorganism Streptomyces avermitilis.";
RL   Nat. Biotechnol. 21:526-531(2003).
CC   -!- FUNCTION: Catalyzes the oxidative demethylation of sarcosine (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: Sarcosine + H(2)O + O(2) = glycine +
CC       formaldehyde + H(2)O(2).
CC   -!- COFACTOR: Binds 1 FAD per subunit (By similarity).
CC   -!- SUBUNIT: Monomer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the MSOX/MTOX family. MSOX subfamily.
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DR   EMBL; BA000030; BAC74661.1; -; Genomic_DNA.
DR   RefSeq; NP_828126.1; -.
DR   HSSP; P40859; 1EL5.
DR   GeneID; 1211626; -.
DR   GenomeReviews; BA000030_GR; SAV_6950.
DR   KEGG; sma:SAV6950; -.
DR   NMPDR; fig|227882.1.peg.6952; -.
DR   HOGENOM; Q827H4; -.
DR   BioCyc; SAVE227882:SAV6950-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:HAMAP.
DR   GO; GO:0008115; F:sarcosine oxidase activity; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_00516; -; 1.
DR   InterPro; IPR006076; FAD-dep_OxRdtase.
DR   Pfam; PF01266; DAO; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; FAD; Flavoprotein; Oxidoreductase.
FT   CHAIN         1    384       Monomeric sarcosine oxidase.
FT                                /FTId=PRO_0000213763.
FT   NP_BIND       6     36       FAD (Potential).
FT   MOD_RES     315    315       S-8alpha-FAD cysteine (By similarity).
SQ   SEQUENCE   384 AA;  42167 MW;  28302788BB0DE585 CRC64;
     MSPTYDVIVI GLGGMGSAAA HHLSARGARV LGLEKFGPVH NRGSSHGGSR ITRQSYFEDP
     AYVPLLLRSY ELYEEVERST GREVATLSGG VMVGRPDSLT VAGSLRSATQ WDLPHEMLDA
     KEIRRRFPTL NPSNDEVALY EKKAGLVRPE NMVAAHLQLA TRQGAELHFE EPMTRWEPYR
     DGVRVHTAEN TYTAGQLVIC PGAWAPQLLT DLGVPFTIER QVMYWFQPRH GVGPFRPENH
     PIYIWEDAEG VQVYGFPSID GPDLGAKVAF FRKGVVCTPE TIDRTVHDHE VQAMADHMSR
     CIPDLPGTFL KAATCMYSNT PDEHFVIARH PAHPDSVTVA CGFSGHGFKF VPVVGEIVAD
     LALTGTTAHP IGLFDPRRLA AAPA
//